Usefulness of length heterogeneity-PCR for monitoring lactic acid bacteria succession during maize ensiling: LAB succession in maize silage by LH-PCR
Open accessAbstract
The use of length-heterogeneity PCR was explored to monitor lactic acid bacteria succession during ensiling of maize. Bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. A length heterogeneity PCR profile database of lactic acid bacteria isolated from the silage and identified by 16S rRNA gene sequencing was established. Although interoperonic 16S rRNA gene length polymorphisms were detected in some isolates, strain analysis showed that most of the lactic acid bacteria species thriving in silage could be discriminated by this method. The length heterogeneity PCR profiles of bacterial communities during maize fermentation were compared with those on a database. Under optimal fermentation conditions all the ecological indices of bacterial diversity, richness and evenness, deduced from community profiles, increased until day thirteen of fermentation and then decreased to the initial values. Pediococcus and Weissella dominated, especially in the first days of fermentation. Lactococcus lactis ssp. lactis and Lactobacillus brevis were mainly found after six days of fermentation. A peak corresponding to Lactobacillus plantarum was present in all the fermentation phases, but was only a minor fraction of the population. Unsuitable fermentation conditions and withered maize leaves in the presence of oxygen and water excess caused an enrichment of Enterococcus sp. and Enterobacter sp.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Brusetti et al. observed a population shift from Pediococcus and Weissella on the first days of fermentation to Enterobacter as the dominant species after 30 days in corn silage.
“After 1, 2, 6, 13, 20, and 30 days of fermentation, they observed a population shift from Pediococcus and Weissella on the first days of fermentation to Enterobacter as the dominant species after 30 days.”
Brusetti et al. reported the presence of B. megaterium, Weissella kimchii, and Enterococcus flavescens in the ensiling of maize.
“Brusetti et al. (2006) reported the presence of B. megaterium in the ensiling of maize, as well as Weissella kimchii and Enterococcus flavescens.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Brusetti et al. 2006
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669 · see citing works
PAST: PALEONTOLOGICAL STATISTICAL SOFTWARE PACKAGE FOR EDUCATION AND DATA ANALYSIS
Hammer, Ø., Harper, D. A. T., & Ryan, P. D. (2001) · Palaeontologia Electronica · Cited by 17,871 · see citing works
Ecological Diversity and Its Measurement
Magurran, A. E. (1988) · Springer eBooks · doi:10.1007/978-94-015-7358-0 · Cited by 10,986 · see citing works
Current protocols in molecular biology
Dudley, R. K. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80264-3 · Cited by 6,037 · see citing works
Fisher, M. M., & Triplett, E. W. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.10.4630-4636.1999 · Cited by 843 · see citing works
Acetic acid increases stability of silage under aerobic conditions
Danner, H., Holzer, M., Mayrhuber, E., & Braun, R. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.562-567.2003 · Cited by 515 · see citing works
New trends and opportunities in the development and use of inoculants for silage
Weinberg, Z. G., & Muck, R. E. (1996) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1996.tb00253.x · Cited by 509 · see citing works
Cardinale, M., Brusetti, L., Quatrini, P., et al. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.10.6147-6156.2004 · Cited by 507 · see citing works
Self-citationRanjard, L., Poly, F., Lata, J., Mougel, C., Thioulouse, J., & Nazaret, S. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.10.4479-4487.2001 · Cited by 481 · see citing works
Suzuki, M. T., Rappé, M. S., & Giovannoni, S. J. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.11.4522-4529.1998 · Cited by 382 · see citing works
Ritchie, N. J., Schutter, M. E., Dick, R. P., & Myrold, D. D. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.4.1668-1675.2000 · Cited by 245 · see citing works
Ennahar, S., Cai, Y., & Fujita, Y. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.444-451.2003 · Cited by 215 · see citing works
The role of Lactobacillus buchneri in forage preservation
Holzer, M., Mayrhuber, E., Danner, H., & Braun, R. (2003) · Trends in biotechnology · doi:10.1016/s0167-7799(03)00106-9 · Cited by 193 · see citing works
Cai, Y., KUMAI, S., OGAWA, M., Benno, Y., & Nakase, T. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.2901-2906.1999 · Cited by 179 · see citing works
Daffonchio, D. G., Borin, S., Frova, G., Manachini, P. L., & Sorlini, C. (1998) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-48-1-107 · Cited by 143 · see citing works
Self-citationCorroler, D., Mangin, I., Desmasures, N., & Gueguen, M. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.12.4729-4735.1998 · Cited by 142 · see citing works
Urzı̀, C., Brusetti, L., Salamone, P., et al. (2001) · Environmental Microbiology · doi:10.1046/j.1462-2920.2001.00217.x · Cited by 131 · see citing works
Self-citationBrusetti, L., Francia, P., Bertolini, C., Pagliuca, A., Borin, S., Sorlini, C., Abruzzese, A., Sacchi, G. A., Viti, C., Giovannetti, L., et al. (2005) · Plant and Soil · doi:10.1007/s11104-005-5399-x · Cited by 125 · see citing works
Self-citationEpiphytic Microflora on Alfalfa and Whole-Plant Corn
Lin, C., Bolsen, K. K., Brent, B. E., Hart, R. A., Dickerson, J. T., Feyerherm, A. M., & Aimutis, W. R. (1992) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(92)78010-2 · Cited by 112 · see citing works
Johnson, L., Harrison, J. H., Davidson, D. L., Robutti, J. L., Swift, M. L., Mahanna, W. C., & Shinners, K. J. (2002) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(02)74143-x · Cited by 110 · see citing works
Lazzi, C., Rossetti, L., Zago, M., Neviani, E., & Giraffa, G. (2004) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2004.02180.x · Cited by 85 · see citing works
Johnson, L., Harrison, J. H., Davidson, D. L., Mahanna, W. C., & Shinners, K. J. (2003) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(03)73607-8 · Cited by 63 · see citing works
Fitzsimons, A., Hols, P., Jore, J., et al. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.10.3529-3535.1994 · Cited by 59 · see citing works
Assessment of Pediococcus acidilactici as a Potential Silage Inoculant
Fitzsimons, A., Duffner, F. M., Curtin, D., Brophy, G., O’Kiely, P., & O'Connell, M. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.9.3047-3052.1992 · Cited by 52 · see citing works
Fenlon, D. R., & Wilson, J. C. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2000.00679.x · Cited by 50 · see citing works
Microbial and chemical changes during the spontaneous ensilage of grape pomace
Piña, C., & Hogg, T. A. (1999) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1999.00726.x · Cited by 39 · see citing works
Efficient Improvement of Silage Additives by Using Genetic Algorithms
Davies, Z., Gilbert, R. J., Merry, R. J., Kell, D. B., Theodorou, M. K., & Griffith, G. W. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.4.1435-1443.2000 · Cited by 37 · see citing works
Growth and Survival of Genetically Manipulated Lactobacillus plantarum in Silage
Sharp, R., O’Donnell, A. G., Gilbert, H., et al. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.8.2517-2522.1992 · Cited by 34 · see citing works
Construction and use of a computerized DNA fingerprint database for lactic acid bacteria from silage
Chan, R. K., Wortman, C. R., Smiley, B. K., et al. (2003) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(03)00186-6 · Cited by 16 · see citing works
Kim, S., Fung, D. Y. C., & Kang, D. (2001) · Journal of Rapid Methods & Automation in Microbiology · doi:10.1111/j.1745-4581.2001.tb00240.x · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Recent advances in silage microbiology
Muck, R. (2013) · Agricultural and Food Science · doi:10.23986/afsci.6718 · Cited by 215 · see citing works
Gatti, M., Bottari, B., Lazzi, C., Neviani, E., & Mucchetti, G. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-7187 · Cited by 216 · see citing works
Zhou, Y., Drouin, P., & Lafrenière, C. (2016) · Journal of Applied Microbiology · doi:10.1111/jam.13198 · Cited by 126 · see citing works
Fabiszewska, A. U., Zielińska, K. J., & Wróbel, B. (2019) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2649-2 · Cited by 121 · see citing works
Dolci, P., Tabacco, E., Cocolin, L., & Borreani, G. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05050-11 · Cited by 108 · see citing works
Romero, J. J., Zhao, Y., Balseca-Paredes, M. A., Tiezzi, F., Gutierrez-Rodriguez, E., & Castillo, M. S. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-11642 · Cited by 105 · see citing works
Gatti, M., De Dea Lindner, J., De Lorentiis, A., Bottari, B., Santarelli, M., Bernini, V., & Neviani, E. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00871-08 · Cited by 94 · see citing works
Drouin, P., Tremblay, J., & Chaucheyras-Durand, F. (2019) · Microorganisms · doi:10.3390/microorganisms7120595 · Cited by 71 · see citing works
Survey on the community and dynamics of lactic acid bacteria in Grana Padano cheese.
Santarelli, M., Bottari, B., Lazzi, C., Neviani, E., & Gatti, M. (2013) · Systematic and Applied Microbiology · doi:10.1016/j.syapm.2013.04.007 · Cited by 56 · see citing works
Study of the bacterial diversity of foods: PCR-DGGE versus LH-PCR
Garofalo, C., Bancalari, E., Milanović, V., Cardinali, F., Osimani, A., Sardaro, M. L. S., Bottari, B., Bernini, V., Aquilanti, L., Clementi, F., et al. (2017) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2016.11.008 · Cited by 49 · see citing works
McEniry, J., O’Kiely, P., Clipson, N. J. W., Forristal, P. D., & Doyle, E. M. (2010) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2009.04557.x · Cited by 38 · see citing works
- Authors
- Brusetti, L., Borin, S., Mora, D., Rizzi, A., Raddadi, N., Sorlini, C., & Daffonchio, D.
- Affiliations
- University of Milan (IT)
- Corresponding author
- Daffonchio, Daniele
- Venue
- FEMS Microbiology Ecology
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-04
- Volume
- 56
- Issue
- 1
- Pages
- 154-164
- ISSN
- 0168-6496 (ISSN-L)
- 1574-6941
- OpenAlex
- W1770718131
- Cited by
- 64
- References
- 30
- Citations by year
- 2023: 2 · 2022: 3 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 6 · 2013: 6 · 2012: 2
- FWCI
- 10.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Ecology (publishedVersion)
- PubMed (publishedVersion)
- Open Research (University of Surrey) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Keywords
- lactic acid bacteria, corn silage, silage fermentation, bacterial community succession, bacterial diversity, Weissella, Lactococcus lactis, Lactobacillus brevis, Lactobacillus plantarum, Enterococcus, Enterobacter, silage spoilage, microbial community analysis
- MeSH terms
- Base Sequence, Zea mays, DNA, Bacterial, Lactobacillus, RNA, Ribosomal, 16S, Silage, Colony Count, Microbial, Polymerase Chain Reaction, DNA Fingerprinting, Sequence Analysis, DNA
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
