Cited by 56Probiotics and Fermented FoodsFermentation and Sensory AnalysisFood and Agricultural Sciences

Survey on the community and dynamics of lactic acid bacteria in Grana Padano cheese.

Santarelli, Marcela, Bottari, Benedetta, Lazzi, Camilla, Neviani, Erasmo, Gatti, Monica·2013·Systematic and Applied Microbiology

Abstract

Grana Padano (GP) is a Protected Designation of Origin cheese made with raw milk and natural whey culture (NWC) that is characterised by a long ripening period. In this study, six GP productions were considered in order to evaluate the trend of microbial dynamics and compare lactic acid bacteria (LAB) population levels in cheeses during the entire cheese-making process. To reach this goal, for each GP production, samples of vat raw milk, NWC and cheeses at 48h, 2, 6, 9 and 13 months were subjected to plate counts and direct counts by fluorescence microscopy, as well as amplicon length heterogeneity-PCR (LH-PCR). Statistical analysis was applied to the results and ecological indices were estimated. It was demonstrated that the LAB able to grow in the cheese-environment conditions could arise from both raw milk and NWC. Starter lactobacilli (SLAB) from NWC were the main species present during acidification, and non-starter LAB (NSLAB), mainly from milk but also from NWC, were able to grow after brining and they dominated during ripening. The peak areas of LH-PCR profiles were used to determine ecological indices during manufacture and ripening. Among cheese ecosystems with different ageing times, diversity, Evenness and Richness were different, with highest bacterial growth and diversity occurring in cheese ripening at 2 months. At this time point, which seemed to be a crucial moment for GP microbial evolution, cell lysis of both SLAB and NSLAB was also observed. Sampling modality and statistical analysis gave greater significance to the results used to describe the microbiological characteristics of a cheese recognised worldwide.

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Crossref · 10.1016/j.syapm.2013.04.007

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Authors
Santarelli, M., Bottari, B., Lazzi, C., Neviani, E., & Gatti, M.
Affiliations
University of Parma (IT)
Corresponding author
Gatti, Monica
Venue
Systematic and Applied Microbiology
Publisher
Elsevier BV
Published
2013-12
Volume
36
Issue
8
Pages
593-600
ISSN
0723-2020 (ISSN-L)
OpenAlex
W2054520193
Cited by
56
References
33
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 2 · 2021: 7 · 2020: 7 · 2019: 1 · 2018: 8 · 2017: 7 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 2
FWCI
5.97
Type
journal article
Language
en
Open access
closed
Locations
  • Systematic and Applied Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Università degli Studi di Parma
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
Grana Padano cheese, lactic acid bacteria, cheese ripening, raw milk, microbial dynamics, bacterial diversity, ecological indices, brining, cell lysis, cheese microbiology
MeSH terms
Cheese, DNA, Bacterial, Genetic Variation, Polymerase Chain Reaction, Lactobacillales, Biota, Bacterial Load

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