Cited by 185Oral microbiology and periodontitis researchProteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins Research

Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin

Wright, Damian P, Rosendale, Douglas I, Roberton, Anthony M·2000·FEMS Microbiology Letters

Abstract

Mucin desulfation is believed to be a rate-limiting step in mucin degradation by colon bacteria. The activities of enzymes hydrolysing nine linkages found in mucin oligosaccharide chains were measured using model substrates, in extracts of two mucin-degrading bacteria, Prevotella strain RS2 and Bacteroides fragilis. Sulfatases desulfating N-acetylglucosamine-6-sulfate, galactose-6-sulfate and galactose-3-sulfate were found. The genomic DNA downstream from the gene encoding the mucin-desulfating sulfatase (N-acetylglucosamine-6-sulfatase) in Prevotella was sequenced, and two putative genes identified which are likely to be coexpressed with this sulfatase, though their activities are unknown. Northern and Western analyses showed that expression of this short operon of three genes is increased during growth on mucin.

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Authors
Wright, D. P., Rosendale, D. I., & Roberton, A. M.
Affiliations
University of Auckland (NZ)
Corresponding author
Roberton, Anthony M
Venue
FEMS Microbiology Letters
Published
2000-09
Volume
190
Issue
1
Pages
73-79
ISSN
  • 0378-1097 (ISSN-L)
  • 1574-6968
OpenAlex
W2070404820
Cited by
185
References
33
Citations by year
2026: 9 · 2025: 17 · 2024: 12 · 2023: 18 · 2022: 22 · 2021: 15 · 2020: 18 · 2019: 7 · 2018: 8 · 2017: 7 · 2016: 10 · 2015: 7 · 2014: 8 · 2013: 4 · 2012: 6
FWCI
1.83
Type
journal article
Language
en
Open access
closed
Locations
  • FEMS Microbiology Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
Auckland Medical Research Foundation
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
Keywords
Prevotella, mucin degradation, sulfatase, Bacteroides fragilis, gene operon, gene expression regulation
MeSH terms
Animals, Bacteroides fragilis, Carbohydrate Sequence, Humans, Molecular Sequence Data, Mucins, Oligosaccharides, Operon, RNA, Messenger, Sulfatases, Blotting, Western, Prevotella

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