Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin
Abstract
Mucin desulfation is believed to be a rate-limiting step in mucin degradation by colon bacteria. The activities of enzymes hydrolysing nine linkages found in mucin oligosaccharide chains were measured using model substrates, in extracts of two mucin-degrading bacteria, Prevotella strain RS2 and Bacteroides fragilis. Sulfatases desulfating N-acetylglucosamine-6-sulfate, galactose-6-sulfate and galactose-3-sulfate were found. The genomic DNA downstream from the gene encoding the mucin-desulfating sulfatase (N-acetylglucosamine-6-sulfatase) in Prevotella was sequenced, and two putative genes identified which are likely to be coexpressed with this sulfatase, though their activities are unknown. Northern and Western analyses showed that expression of this short operon of three genes is increased during growth on mucin.
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Unfavorable taxa had more genes for LPS synthesis and mucus-degrading enzymes like alpha-l-fucosidase and alpha-galactosidase.
“Conversely, within unfavorable taxa, genes encoding enzymes related to LPS synthesis and mucus degradation such as alpha-l-fucosidase45 and alpha-galactosidase46 were increased.”
From Intestinal microbiota signatures of clinical response and immune-related adverse events in melanoma patients treated with anti-PD-1 (2022) · cites this paper as [46]
Bacterial genes for alpha-l-fucosidase, alpha-galactosidase and glycosyltransferases were enriched in non-responders.
“Using a new binning approach to focus on candidate bacterial driver genes, we observed enrichment of bacterial genes encoding key enzymes: polysaccharide deacetylase (associated with reduction of polysaccharide immunogenicity42) in Rs; and alpha-l-fucosidase45 and alpha-galactosidase46 (associated with mucus degradation) and glycosyltransferases (associated with LPS synthesis57) in NRs.”
From Intestinal microbiota signatures of clinical response and immune-related adverse events in melanoma patients treated with anti-PD-1 (2022) · cites this paper as [46]
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- Authors
- Wright, D. P., Rosendale, D. I., & Roberton, A. M.
- Affiliations
- University of Auckland (NZ)
- Corresponding author
- Roberton, Anthony M
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-09
- Volume
- 190
- Issue
- 1
- Pages
- 73-79
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2070404820
- Cited by
- 185
- References
- 33
- Citations by year
- 2026: 9 · 2025: 17 · 2024: 12 · 2023: 18 · 2022: 22 · 2021: 15 · 2020: 18 · 2019: 7 · 2018: 8 · 2017: 7 · 2016: 10 · 2015: 7 · 2014: 8 · 2013: 4 · 2012: 6
- FWCI
- 1.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Auckland Medical Research Foundation
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- Prevotella, mucin degradation, sulfatase, Bacteroides fragilis, gene operon, gene expression regulation
- MeSH terms
- Animals, Bacteroides fragilis, Carbohydrate Sequence, Humans, Molecular Sequence Data, Mucins, Oligosaccharides, Operon, RNA, Messenger, Sulfatases, Blotting, Western, Prevotella
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