Biochemistry and molecular biology of exocellular fungal β-(1,3)- and β-(1,6)-glucanases
Open accessAbstract
Many fungi produce exocellular beta-glucan-degrading enzymes, the beta-glucanases including the noncellulolytic beta-(1,3)- and beta-(1,6)-glucanases, degrading beta-(1,3)- and beta-(1,6)-glucans. An ability to purify several exocellular beta-glucanases attacking the same linkage type from a single fungus is common, although unlike the beta-1,3-glucanases, production of multiple beta-1,6-glucanases is quite rare in fungi. Reasons for this multiplicity remain unclear and the multiple forms may not be genetically different but arise by posttranslational glycosylation or proteolytic degradation of the single enzyme. How their synthesis is regulated, and whether each form is regulated differentially also needs clarifying. Their industrial potential will only be realized when the genes encoding them are cloned and expressed in large quantities. This review considers what is known in molecular terms about their multiplicity of occurrence, regulation of synthesis and phylogenetic diversity. It discusses how this information assists in understanding their functions in the fungi producing them. It deals largely with exocellular beta-glucanases which here refers to those recoverable after the cells are removed, since those associated with fungal cell walls have been reviewed recently by Adams (2004). It also updates the earlier review by Pitson et al. (1993).
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Hydrolysis of laminarin and chrysolaminarin is catalyzed by four enzymes: endo-β-(1→6) glucanases, endo-β-(1→3) glucanases, exo-β-(1→3) glucanases, and β-glucosidases.
“Hydrolysis of laminarin and chrysolaminarin is catalyzed by the action of four enzymes: endo-β-(1→6) glucanases (EC 3.2.1.75),endo-β-(1→3) glucanases (EC 3.2.1.39),exo-β-(1→3) glucanases (EC 3.2.1.58),and β-glucosidases (EC 3.2.1.21).”
From The Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol (2016) · cites this paper as Martin et al., 2007
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- Authors
- Martin, K., McDougall, B. M., McIlroy, S., Jayus, Chen, J., & Seviour, R. J.
- Affiliations
- University of Dundee (GB)
- La Trobe University (AU)
- Venue
- FEMS Microbiology Reviews
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-03
- Volume
- 31
- Issue
- 2
- Pages
- 168-192
- ISSN
- 0168-6445 (ISSN-L)
- 1574-6976
- OpenAlex
- W1972589860
- Cited by
- 127
- References
- 147
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 8 · 2022: 11 · 2021: 11 · 2020: 9 · 2019: 7 · 2018: 6 · 2017: 6 · 2016: 7 · 2015: 5 · 2014: 6 · 2013: 8 · 2012: 3
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Microbiology Reviews (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- β-glucanase, β-1,3-glucanase, β-1,6-glucanase, extracellular enzymes, fungal enzymes, post-translational glycosylation, proteolysis, enzyme regulation, gene cloning, industrial enzymes, phylogenetic diversity, fungal cell wall
- MeSH terms
- Amino Acid Sequence, Binding Sites, Carbon, Cell Wall, Fermentation, Fungi, Glycoside Hydrolases, Introns, Molecular Sequence Data, Nitrogen, Phylogeny, Conserved Sequence, Amino Acid Motifs, Protein Sorting Signals, Glucan 1,3-beta-Glucosidase, beta-Glucans
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