Fungal ß(1,3)-D-glucan synthesis
Abstract
The polysaccharide beta(1,3)-D-glucan is a component of the cell wall of many fungi. Synthesis of the linear polymer is catalysed by UDP-glucose beta(1,3)-D-glucan beta(3)-D-glucosyltransferase. Because this enzyme has a key role in fungal cell-wall synthesis, and because many organisms that are responsible for human mycoses, including Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans, produce walls that are rich in beta(1,3)-glucan, it has been and remains the focus of intensive study. From early characterization of the enzymatic activity in Saccharomyces cerevisiae, advances have been made in purification of the enzyme, identification of essential subunits and description of regulatory circuitry that controls expression and localization of different components of the multisubunit enzyme complex. Progress in each of these areas has been enhanced dramatically by the availability of specific inhibitors of the enzymatic reaction that produces beta(1,3)-glucan. These natural product inhibitors have utility both as tools to dissect the biology of beta(1,3)-glucan synthase and as sources for development of semisynthetic derivatives with clinical utility in treatment of human fungal disease. This review will focus on the biochemistry, genetics and regulation of the enzyme.
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It has only been conclusively demonstrated that FKS mutations are sufficient to confer echinocandin resistance in S. cerevisiae.
“While there is an association of FKS mutations with resistance, and biochemical data support the resistance mechanism, it has only been conclusively demonstrated that such mutations are sufficient to confer echinocandin resistance in S. cerevisiae [82], [83].”
From Global Analysis of the Evolution and Mechanism of Echinocandin Resistance in Candida glabrata (2012) · cites this paper as [82]
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- Authors
- Douglas, C. M.
- Affiliations
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Corresponding author
- Douglas, C. M.
- Venue
- Medical Mycology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-01
- Volume
- 39
- Issue
- 1
- Pages
- 55-66
- ISSN
- 0268-1218 (ISSN-L)
- 1369-3786
- 1460-2709
- OpenAlex
- W2143508569
- Cited by
- 312
- References
- 100
- Citations by year
- 2026: 14 · 2025: 17 · 2024: 16 · 2023: 12 · 2022: 12 · 2021: 13 · 2020: 22 · 2019: 8 · 2018: 14 · 2017: 9 · 2016: 8 · 2015: 10 · 2014: 16 · 2013: 15 · 2012: 19
- FWCI
- 0.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medical Mycology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- β-1,3-glucan, fungal cell wall, enzyme regulation, enzyme subunits, enzyme inhibitors, Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, Saccharomyces cerevisiae, human fungal diseases
- MeSH terms
- Fungal Proteins, Fungi, Glucans, Glucosyltransferases, Membrane Proteins, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae Proteins, beta-Glucans, Echinocandins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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