Selective detection of superoxide anion radicals generated from macrophages by using a novel fluorescent probe
Abstract
Quantitation of superoxide radical (O 2–·) production at the site of radical generation remains challenging. A simple method to detect nanomolar to micromolar levels of superoxide radical in aqueous solution has been developed and optimized. This method is based on the efficient trapping of O 2–· using a novel fluorescent probe (2‐chloro‐1,3‐dibenzothiazolinecyclohexene), coupled with a spectra character‐signaling increase event. A high‐specificity and high‐sensitivity fluorescent probe was synthesized in‐house and used to image O 2–· in living cells. Better selectivity for O 2–· over competing cellular reactive oxygen species and some biological compounds illustrates the advantages of our method. Under optimal conditions, the linear calibration range for superoxide anion radicals was 5.03 × 10−9−3.33 × 10−6 m. The detection limit was 1.68 × 10−9 m. Fluorescence images of probe‐stained macrophages stimulated with 4β‐phorbol 12‐myristate 13‐acetate were obtained successfully using a confocal laser scanning microscope.
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The fast-response fluorescent probe 2,2′-(2-chloro-1,3-phenylene) bis (2,3-dihydrobenzo [d] thiazole) operates by a similar detection mechanism in the presence of O2•− and shows high selectivity towards O2•− over other ROS.
“The same research group also developed the fast-response fluorescent probe 2,2′-(2-chloro-1,3-phenylene) bis (2,3-dihydrobenzo [d] thiazole) which operates by a similar detection mechanism in the presence of O2•− and also shows a high selectivity towards O O2•− in contrast to other ROS [160].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [160]
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Self-citation
- Authors
- Gao, J. J., Xu, K. H., Tang, B., Yin, L. L., Yang, G. W., & An, L. G.
- Affiliations
- Shandong Normal University (CN)
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2007-03-09
- Volume
- 274
- Issue
- 7
- Pages
- 1725-1733
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- OpenAlex
- W2123791876
- Cited by
- 76
- References
- 17
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 7 · 2022: 3 · 2021: 8 · 2020: 4 · 2019: 8 · 2018: 5 · 2017: 7 · 2016: 5 · 2015: 5 · 2014: 2 · 2013: 6 · 2012: 3
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- superoxide, fluorescent probes, superoxide detection, macrophages, reactive oxygen species, live-cell imaging, confocal laser scanning microscopy, PMA stimulation
- MeSH terms
- Animals, Catalase, Cell Line, Fluorescent Dyes, Hydrogen-Ion Concentration, Kinetics, Macrophages, Microscopy, Fluorescence, Models, Chemical, Spectrometry, Fluorescence, Superoxides, Superoxide Dismutase, Tetradecanoylphorbol Acetate, Xanthine Oxidase, Molecular Structure, Reactive Oxygen Species, Microscopy, Confocal, Xanthine
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