The Phototumorigenic Fluoroquinolone Lomefloxacin Photosensitizes Pyrimidine Dimer Formation in Human Keratinocytes In Vitro
Abstract
The fluoroquinolone antibiotic lomefloxacin is phototoxic, photogenotoxic, photomutagenic and photosensitizes tu‐morigenesis in mouse skin. We have used T4 endonucle‐ase V to demonstrate that lomefloxacin photosensitizes pyrimidine dimer formation in a human keratinocyte line (HaCaT). A possible mechanism for this effect would be triplet‐triplet energy transfer. However, there is indirect evidence that the lomefloxacin triplet yield is very low, making this reaction less likely. The finding that lomefloxacin photosensitizes production of highly mutagenic pyrimidine dimers correlates with its ability to initiate skin tumor formation in mice. Until the potential of other fluoroquinolones to photosensitize dimer formation is explored it may be unadvisable to prescribe these antibiotics to patients with defective DNA repair capacity (e.g. xeroderma pigmentosum).
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
UVA-excited lomefloxacin generates cyclobutane pyrimidine dimers in human keratinocytes, detected as T4 endonuclease V-sensitive sites.
“Thus, UVA-excited lomefloxacin has been found to generate in human keratinocytes CPDs that were revealed as T4 endonuclease V-sensitive sites (149).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [149]
UVA-sensitized formation of cyclobutane pyrimidine dimers by fluoroquinolones occurs in the DNA of isolated cells and mouse skin.
“Several examples of UVA-sensitized formation of CPDs by fluoroquinolones in the DNA of isolated cells (93,149,150) and mouse skin (151) are available.”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [149]
Cites
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.3.761 · Cited by 4,170 · see citing works
Sunburn and p53 in the onset of skin cancer
Ziegler, A., Jonason, A. S., Leffellt, D. J., Simon, J. A., Sharma, H. W., Kimmelman, J., Remington, L., Jacks, T., & Brash, D. E. (1994) · Nature · doi:10.1038/372773a0 · Cited by 1,497 · see citing works
Collins, A. R., Aiguo, M., & Duthie, S. J. (1995) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(94)00043-6 · Cited by 699 · see citing works
The comet assay: mechanisms and technical considerations
Klaude, M., Eriksson, S., Nygren, J., & Ahnström, G. (1996) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(95)00063-1 · Cited by 539 · see citing works
Single-cell Gel Electrophoresis Applied to the Analysis of UV-C Damage and Its Repair in Human Cells
Gedik, C. M., Ewen, S. W. B., & Collins, A. R. (1992) · International Journal of Radiation Biology · doi:10.1080/09553009214552161 · Cited by 355 · see citing works
Fluoroquinolone antibacterials enhance UVA-induced skin tumors
Klecak, G., Urbach, F., & Urwyler, H. (1997) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(96)07424-6 · Cited by 203 · see citing works
Biological Responses to Ultraviolet A Radiation
Urbach, F. (1992) · Medical Entomology and Zoology · Cited by 147 · see citing works
Chételat, A.-A., Albertini, S., & Gocke, E. (1996) · Mutagenesis · doi:10.1093/mutage/11.5.497 · Cited by 119 · see citing works
Photogeneration of Fluoride by the Fluoroquinolone Antimicrobial Agents Lomefloxacin and Fleroxacin
Martínez, L. J., Li, G., & Chignell, C. F. (1997) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1997.tb08612.x · Cited by 115 · see citing works
Photocleavage of DNA by the fluoroquinolone antibacterials
Martínez, L. J., & Chignell, C. F. (1998) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(98)00160-2 · Cited by 76 · see citing works
Quinolone antibiotic with potential to photosensitize skin tumorigenesis
Johnson, B. E., Gibbs, N. K., & Ferguson, J. (1997) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/s1011-1344(96)07423-4 · Cited by 68 · see citing works
Self-citationFluoroquinolone phototoxicity: a comparison of moxifloxacin and lomefloxacin in normal volunteers
Man, I., Murphy, J., & Ferguson, J. C. (1999) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/43.suppl_2.77 · Cited by 67 · see citing works
Reavy, H. J., Traynor, N. J., & Gibbs, N. K. (1997) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1997.tb03160.x · Cited by 63 · see citing works
Self-citationFASANI, E., Mella, M., Caccia, D., et al. (1997) · Chemical Communications · doi:10.1039/a703226i · Cited by 63 · see citing works
EXCITED STATE PRECURSORS OF THYMINE PHOTODIMERS
Lamola, A. A. (1968) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1968.tb08044.x · Cited by 60 · see citing works
Nonsteroidal antiinflammatory drug-photosensitized formation of pyrimidine dimer in DNA
Chouini–Lalanne, N., Defais, M. J., & Paillous, N. (1998) · Biochemical Pharmacology · doi:10.1016/s0006-2952(97)00511-x · Cited by 57 · see citing works
In VitroPhotogenotoxic Activity of Clinafloxacin: A Paradigm Predicting Photocarcinogenicity
Bulera, S. J., Theiss, J. C., Festerling, T. A., et al. (1999) · Toxicology and Applied Pharmacology · doi:10.1006/taap.1999.8653 · Cited by 43 · see citing works
Costalat, R., Blais, J., Ballini, J., Moysan, A., Cadet, J., CHALVET, O., & Vigny, P. (1990) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1990.tb01709.x · Cited by 35 · see citing works
HøNNÅS, P. I., & Steen, H. B. (1970) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1970.tb05972.x · Cited by 31 · see citing works
Study of photochemical fluorescence enhancement of the terbium-lomefloxacin complex
Zhang, T., Huichun, Z., Linpei, J., et al. (1999) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/s1010-6030(98)00450-x · Cited by 10 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Mouret, S., Baudouin, C., Charveron, M., Favier, A., Cadet, J., & Douki, T. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604213103 · Cited by 684 · see citing works
Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
Marrot, L., Belaïdi, J. P., Jones, C., Perez, P., Meunier, J. R., Riou, L., & Sarasin, A. (2003) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2003.12422.x · Cited by 70 · see citing works
Cyclobutane pyrimidine dimers are photosensitised by carprofen plus UVA in human HaCaT cells
Robinson, K. S., Traynor, N. J., Moseley, H., Ferguson, J., & Woods, J. A. (2010) · Toxicology in Vitro · doi:10.1016/j.tiv.2010.03.007 · Cited by 18 · see citing works
Self-citation
- Authors
- Traynor, N. J., & Gibbs, N. K.
- Affiliations
- University of Dundee (GB)
- Corresponding author
- Gibbs, Neil K.
- ORCID
- Gibbs, Neil K.
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 1999-12
- Volume
- 70
- Issue
- 6
- Pages
- 957-959
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- OpenAlex
- W1967532615
- Cited by
- 26
- References
- 20
- Citations by year
- 2021: 2 · 2020: 1 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Funders
- Tenovus
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Keywords
- lomefloxacin, fluoroquinolones, phototoxicity, photomutagenesis, photosensitization, keratinocytes, triplet-triplet energy transfer, T4 endonuclease V, skin carcinogenesis, xeroderma pigmentosum, DNA repair deficiency
- MeSH terms
- Anti-Infective Agents, Cell Line, Transformed, DNA Damage, Humans, Pyrimidine Dimers, Quinolones, Keratinocytes, Photosensitizing Agents, Fluoroquinolones
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
