Cited by 34Skin Protection and AgingBotanical Studies and ApplicationsPhytochemicals and Antioxidant Activities

Protective effects of hawthorn (Crataegus pinnatifida ) polyphenol extract against UVB-induced skin damage by modulating the p53 mitochondrial pathway in vitro and in vivo

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Liu, Suwen, Sui, Qianqian, Zou, Jian, Zhao, Yanxue, Chang, Xuedong·2019·Journal of Food Biochemistry

Abstract

This study investigated the effect of a hawthorn polyphenol extract (HPE) on ultraviolet B (UVB)-induced damage in HaCaT cells and mice. High-performance liquid chromatography/electrospray ionization tandem mass spectrometry was used to analyze the phenolic composition of HPE. The protective effects of HPE and its main components were compared in HaCaT cells. An enzyme-linked immunosorbent assay was used to detect DNA damage (8-hydroxydeoxyguanosine levels). Flow cytometry and western blotting were used to measure the extent of apoptosis and the levels of apoptosis-related proteins, respectively. Treatment with HPE or its polyphenol components inhibited the UVB-induced damage by removing an excess of reactive oxygen species (ROS), reducing DNA damage and p53 activation, regulating the protein expression of B-cell lymphoma 2 family members toward antiapoptotic ratios, and reducing caspase activation. Similar effects were observed in a UVB-irradiated mouse skin, as detected using terminal deoxynucleotidyl transferase dUTP nick-end labeling, immunohistochemistry, and western blotting assays. These results suggest that HPE can be used as a natural dietary supplement for the prevention and treatment of UVB radiation-induced skin damage. PRACTICAL APPLICATIONS: Hawthorn (Crataegus pinnatifida) shows antioxidant, anti-inflammatory, and lipid-lowering effects. As natural, healthy, and effective additives, HPEs have been widely used in food and health products. The results of this study reveal the molecular mechanisms underlying HPE effects, showing that HPE reverses the effects of UVB irradiation via removal of an excess of ROS and reduction of DNA damage and p53 expression in vitro and in vivo. Consequently, HPE upregulates the expression of antiapoptotic BCL-2 and downregulates that of proapoptotic BAX, thereby reducing the activation of caspase-3/9 and inhibiting apoptosis. These findings suggest that HPE can be used as the base ingredient for antiphotoaging food products. This study provides both theoretical and experimental background for hawthorn deep processing and utilization.

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  • Oral treatment with hawthorn polyphenol extract also inhibits skin photoaging through the p53 mitochondrial pathway.

    “This group later demonstrated that oral treatment with hawthorn polyphenol extract also inhibits skin photoaging through the p53 mitochondrial pathway [108].”

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Authors
Liu, S., Sui, Q., Zou, J., Zhao, Y., & Chang, X.
Affiliations
  • Hebei Normal University of Science and Technology (CN)
  • Henan University of Animal Husbandry and Economy (CN)
  • Qingdao University (CN)
Corresponding author
Chang, Xuedong
Venue
Journal of Food Biochemistry
Publisher
Wiley
Published
2018-11-11
Volume
43
Issue
2
Pages
e12708
ISSN
0145-8884 (ISSN-L)
OpenAlex
W2900817997
Cited by
34
References
44
Citations by year
2026: 5 · 2025: 6 · 2024: 4 · 2023: 5 · 2022: 6 · 2021: 3 · 2020: 5
FWCI
2.17
Type
journal article
Language
en
Open access
Locations
  • Journal of Food Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
Science and Technology Support Program of Hebei in China (16237113D)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Botanical Studies and Applications
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Keywords
hawthorn, Crataegus pinnatifida, polyphenolic extracts, UVB-induced skin damage, p53, mitochondrial apoptosis, reactive oxygen species, DNA damage, 8-hydroxy-2'-deoxyguanosine, Bcl-2/Bax ratio, HaCaT keratinocytes, photoprotection, dietary supplements
MeSH terms
Animals, DNA Damage, Female, Fruit, Humans, Mice, Inbred BALB C, Mitochondria, Plant Extracts, Ultraviolet Rays, Skin Aging, Tumor Suppressor Protein p53, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Crataegus, Mice, Polyphenols

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