Hawthorn polyphenol extract inhibits UVB-induced skin photoaging by regulating MMP expression and type i procollagen production in mice
Abstract
Ultraviolet (UV) B radiation can cause skin aging by increasing matrix metalloproteinase (MMP) production and collagen degradation, leading to the formation of wrinkles. This study investigated whether hawthorn polyphenol extract (HPE) protects against UVB-induced skin photoaging using HaCaT human keratinocytes, normal human dermal fibroblasts (HDFs), and mice. Analysis of the phenol composition of HPE by high-performance liquid chromatography-mass spectrometry showed that chlorogenic acid (13.5%), procyanidin B2 (19.2%), and epicatechin (18.8%) collectively accounted for 51.4% of total phenol content and represent the active ingredients of hawthorn fruit. A cell viability assay revealed that HPE treatment promoted cell proliferation in HaCaT cells and HDFs. On the other hand, MMP-1 and type I procollagen production was decreased and increased, respectively, in UVB-exposed cells treated with HPE as compared with those without treatment, as determined by enzyme-linked immunosorbent assay. Hematoxylin and eosin and Weigert staining of dermal tissue specimens from mice demonstrated that HPE also reversed UVB-induced epidermal thickening and dermal damage. The increase in production of reactive oxygen species and decrease in antioxidant enzyme activity as well as the increase in nuclear factor-κB activation and mitogen-activated protein kinase phosphorylation induced by UVB irradiation were reversed by HPE (100 or 300 mg/kg body weight), which also suppressed MMP expression and stimulated the production of type I procollagen in the dorsal skin of UVB-irradiated mice. These results suggest that HPE is a natural product that can prevent UVB radiation-induced skin photoaging.
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Hawthorn polyphenol extract reversed epidermal thickening and dermal damage and promoted production of type I procollagen in dorsal skin of UVB-irradiated mice through inactivation of NF-kappaB and phosphorylation of MAPK.
“Hawthorn polyphenol extract reversed epidermal thickening and dermal damage and promoted the production of type I procollagen in the dorsal skin of UVB-irradiated mice through the inactivation of NF-κB and the phosphorylation of MAPK.”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [107]
Oral administration of hawthorn polyphenol extract protects against UVB-induced facial skin photoaging in female Balb/c mice.
“Chang et al. showed that oral administration of hawthorn polyphenol extract protects against UVB-induced facial skin photoaging in female Balb/c mice.”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [107]
Dietary supplementation with hawthorn polyphenol extract decreased production of ROS and increased antioxidant enzyme activity in mouse dorsal skin.
“In addition, dietary supplementation with hawthorn polyphenol extract decreased the production of ROS and increased the antioxidant enzyme activity in mouse dorsal skin [107].”
From Boosting the Photoaged Skin: The Potential Role of Dietary Components (2021) · cites this paper as [107]
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Self-citation
- Authors
- Liu, S., You, L., Zhao, Y., & Chang, X.
- Affiliations
- Hebei Normal University of Science and Technology (CN)
- Corresponding author
- Chang, Xuedong
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-07-22
- Volume
- 66
- Issue
- 32
- Pages
- 8537-8546
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2883347226
- Cited by
- 84
- References
- 45
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 15 · 2021: 12 · 2020: 13 · 2019: 9 · 2018: 3
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- the Science and Technology Support Program of Hebei in China (16237113D)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- photoaging, UV-B radiation, MMP-1, type I procollagen, collagen degradation, chlorogenic acid, procyanidin B2, epicatechin, keratinocytes, dermal fibroblasts, reactive oxygen species, NF-κB activation, MAPK signaling, antioxidant enzymes, epidermal thickening
- MeSH terms
- Animals, Female, Fibroblasts, Humans, Mice, Inbred BALB C, Mice, Hairless, Plant Extracts, Procollagen, Skin, Ultraviolet Rays, Skin Aging, Keratinocytes, Matrix Metalloproteinase 1, Collagen Type I, Crataegus, Mice, Polyphenols
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