Evaluation of two decellularization methods in the development of a whole‐organ decellularized rat liver scaffold
Abstract
Aim: Hepatic tissue engineering is considered as a possible alternative to liver transplantation for end‐stage liver disease. Several methods of decellularization of xenogeneic liver are available to produce three‐dimensional organ scaffolds for engineering liver tissues. However, rare studies have examined and compared the effectiveness of different methods on the structure and composition of intact decellularized liver extracellular matrix. Methods: Two decellularization methods were adopted herein. Their effects on collagen, elastin, glycosaminoglycans ( GAG s), hepatocyte growth factor ( HGF ) content and influence to the function of hepatocytes cultured in scaffolds were examined and compared. Results: The complete tissue decellularization was successfully achieved after treatment with sodium dodecyl sulphate (SDS) and Triton X‐100. The total absence of nuclear structures and removal of viable cells were confirmed by haematoxylin‐eosin staining and scanning electron microscopy. Collagen was preserved after both treatments. However, the elastin content decreased to about 20% and 60%, the GAGs content decreased to about 10% and 50% and the HGF content decreased to about 20% and 60% of the native liver level after SDS and Triton X‐100 treatment respectively. The Triton X‐100‐treated scaffolds were much superior than SDS‐treated scaffolds in supporting liver‐specific function, including albumin secretion ( P = 0.001), urea synthesis ( P = 0.002), ammonia elimination ( P = 0.007) and mRNA expression levels of drug metabolism enzymes. Conclusion: This study suggested that liver extracellular matrix scaffolds constructed using perfusion of Triton X‐100 as described herein might provide a more effective and ideal material for the usage in tissue engineering and regenerative medicine approaches.
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Utilizing TX100 as a posttreatment in decellularization is more adept at preserving ECM protein components.
“Ren et al. [44] suggested that utilizing TX100 as a posttreatment in the decellularization process is more adept at preserving ECM protein components”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (<i>Oreochromis niloticus</i>) Viscera (2024) · cites this paper as [44]
Agitation at low temperatures in TX100 induces less disruption to the ECM structure.
“indicating that agitation at low temperatures in TX100 induces less disruption to the ECM structure.”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (<i>Oreochromis niloticus</i>) Viscera (2024) · cites this paper as [44]
Triton X-100 was much superior in preservation of the ECM than SDS.
“This study indicated that Triton X-100 was much superior in preservation of the ECM than SDS.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [101]
Several studies have been conducted to evaluate the feasibility of recellularizing decellularized livers, primarily on rodent liver initially, and researchers have continued to scale it up using whole porcine and human livers.
“Several studies have been conducted to evaluate the feasibility of recellularizing decellularized livers, primarily on rodent liver initially, and researchers have continued to scale it up using whole porcine and human livers (Table 1) [8,14,101,110,[193], [194], [195]].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [101]
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“Several studies have been conducted to evaluate the feasibility of recellularizing decellularized livers, primarily on rodent liver initially, and researchers have continued to scale it up using whole porcine and human livers (Table 1) [8,14,101,110,193 – 195].”
BackgroundAron, J., Bual, R., Alimasag, J., Arellano, F., Baclayon, L., Bantilan, Z. C., Lumancas, G., Nisperos, M. J., Labares, M., Valle, K. D. D., et al. (2024) · International Journal of Biomaterials · doi:10.1155/2024/6148496 · Cited by 7 · see citing works
- Authors
- Ren, H., Shi, X., Tao, L., Xiao, J., Han, B., Zhang, Y., Yuan, X., & Ding, Y.
- Affiliations
- Nanjing Drum Tower Hospital (CN)
- Corresponding author
- Ding, Yitao
- Venue
- Liver International
- Publisher
- Wiley
- Published
- 2013-01-10
- Volume
- 33
- Issue
- 3
- Pages
- 448-458
- ISSN
- 0106-9543 (ISSN-L)
- 1478-3223
- 1478-3231
- OpenAlex
- W2006247923
- Cited by
- 127
- References
- 4
- Citations by year
- 2026: 1 · 2025: 9 · 2024: 6 · 2023: 9 · 2022: 12 · 2021: 16 · 2020: 3 · 2019: 15 · 2018: 10 · 2017: 12 · 2016: 10 · 2015: 15 · 2014: 9
- FWCI
- 7.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Liver International (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81170418)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- decellularization, extracellular matrix, Triton X-100, sodium dodecyl sulfate, hepatocyte growth factor, collagen, elastin, glycosaminoglycans, hepatocyte function, albumin secretion, urea synthesis, drug-metabolizing enzymes, liver tissue engineering, regenerative medicine
- MeSH terms
- Animals, Collagen, Elastin, Extracellular Matrix, Glycosaminoglycans, Liver, Sodium Dodecyl Sulfate, Hepatocyte Growth Factor, Octoxynol, Hepatocytes, Tissue Engineering, Regenerative Medicine, Rats, Tissue Scaffolds, End Stage Liver Disease
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