An R2R3‐ MYB transcription factor regulates carotenoid pigmentation in Mimulus lewisii flowers
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Summary: Carotenoids are yellow, orange, and red pigments that contribute to the beautiful colors and nutritive value of many flowers and fruits. The structural genes in the highly conserved carotenoid biosynthetic pathway have been well characterized in multiple plant systems, but little is known about the transcription factors that control the expression of these structural genes. By analyzing a chemically induced mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments, we have identified an R2R3‐ MYB , Reduced Carotenoid Pigmentation 1 ( RCP 1), as the first transcription factor that positively regulates carotenoid biosynthesis during flower development. Loss‐of‐function mutations in RCP 1 lead to down‐regulation of all carotenoid biosynthetic genes and reduced carotenoid content in M. lewisii flowers, a phenotype recapitulated by RNA interference in the wild‐type background. Overexpression of this gene in the rcp1 mutant background restores carotenoid production and, unexpectedly, results in simultaneous decrease of anthocyanin production in some transgenic lines by down‐regulating the expression of an activator of anthocyanin biosynthesis. Identification of transcriptional regulators of carotenoid biosynthesis provides the ‘toolbox’ genes for understanding the molecular basis of flower color diversification in nature and for potential enhancement of carotenoid production in crop plants via genetic engineering.
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Carotenoids are dietary antioxidants that have demonstrated photoprotective activity.
“Carotenoids are dietary antioxidants that have demonstrated photoprotective activity.”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [103]
In plants, carotenoids act as accessory light-harvesting pigments and protect from photooxidative damage.
“In plants, these compounds are components of the photosynthetic machinery where they act as accessory light-harvesting pigments and protect from photooxidative damage [103].”
From Antioxidants from Plants Protect against Skin Photoaging (2018) · cites this paper as [103]
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Antioxidants from Plants Protect against Skin Photoaging
Petruk, G., Del Giudice, R., Rigano, M. M., & Monti, D. M. (2018) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2018/1454936 · Cited by 283 · see citing works
- Authors
- Sagawa, J. M., Stanley, L. E., LaFountain, A. M., Frank, H. A., Liu, C., & Yuan, Y.
- Affiliations
- University of Connecticut (US)
- Corresponding author
- Yuan, Yao‐Wu
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2015-09-17
- Volume
- 209
- Issue
- 3
- Pages
- 1049-1057
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2095905168
- Cited by
- 190
- References
- 64
- Citations by year
- 2026: 10 · 2025: 22 · 2024: 28 · 2023: 26 · 2022: 21 · 2021: 26 · 2020: 14 · 2019: 15 · 2018: 12 · 2017: 11 · 2016: 4 · 2015: 1
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Connecticut
- University of Connecticut Research Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- R2R3-MYB transcription factor, carotenoid biosynthesis, flower color, Mimulus lewisii, flower development, anthocyanin biosynthesis, transcriptional regulation
- MeSH terms
- Anthocyanins, Carotenoids, Mutation, Pigmentation, Plant Proteins, Transcription Factors, Down-Regulation, Genes, Plant, Gene Expression Regulation, Plant, Plants, Genetically Modified, Mimulus, RNA Interference, Flowers, Biosynthetic Pathways, Genetic Association Studies
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