TRICHOME AND ARTEMISININ REGULATOR 2 positively regulates trichome development and artemisinin biosynthesis in Artemisia annua
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Summary: Glandular secretory trichomes (GSTs) are regarded as biofactories for synthesizing, storing, and secreting artemisinin. It is necessary to figure out the initiation and development regulatory mechanism of GSTs to cultivate high‐yielding Artemisia annua. Here, we identified an MYB transcription factor, AaTAR2, from bioinformatics analysis of the A. annua genome database and Arabidopsis trichome development‐related genes. AaTAR2 is mainly expressed in young leaves and located in the nucleus. Repression and overexpression of AaTAR2 resulted in a decrease and increase, respectively, in the GSTs numbers, leaf biomass, and the artemisinin content in transgenic plants. Furthermore, the morphological characteristics changed obviously in trichomes, suggesting AaTAR2 plays a key role in trichome formation. In addition, the expression of flavonoid biosynthesis genes and total flavonoid content increased dramatically in AaTAR2‐overexpressing transgenic plants. Owing to flavonoids possibly counteracting emerging resistance to artemisinin in Plasmodium species, AaTAR2 is a potential target to improve the effect of artemisinin in clinical therapy. Taken together, AaTAR2 positively regulates trichome development and artemisinin and flavonoid biosynthesis. A better understanding of this ‘multiple functions’ transcription factor may enable enhanced artemisinin and flavonoids yield. AaTAR2 is a potential breeding target for cultivating high‐quality A. annua.
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AaTAR2 acts as a positive regulator of glandular trichome initiation and development, and its over-expression resulted in increased artemisinin content.
“Zhou et al. (2020) identified an MYB transcription factor, AaTAR2 which acts as a positive regulator of glandular trichome initiation and development, and its over-expression resulted in increased artemisinin content.”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Zhou et al. 2020
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Wani, K. I., Choudhary, S., Zehra, A., Naeem, M., Weathers, P., & Aftab, T. (2021) · Planta · doi:10.1007/s00425-021-03676-3 · Cited by 74 · see citing works
- Authors
- Zhou, Z., Tan, H., Li, Q., Li, Q., Wang, Y., Bu, Q., Li, Y., Wu, Y., Chen, W., & Zhang, L.
- Affiliations
- Second Military Medical University (CN)
- Shanghai University (CN)
- Corresponding author
- Chen, Wansheng
- Zhang, Lei
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2020-08-02
- Volume
- 228
- Issue
- 3
- Pages
- 932-945
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W3037779720
- Cited by
- 83
- References
- 47
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 16 · 2023: 21 · 2022: 15 · 2021: 7
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31670292, 31970316, 81673529)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Keywords
- Artemisia annua, artemisinin biosynthesis, trichome development, MYB transcription factors, flavonoid biosynthesis, transgenic overexpression, artemisinin resistance, Plasmodium, medicinal plant breeding
- MeSH terms
- Plant Breeding, Plant Proteins, Plants, Genetically Modified, Artemisia annua, Artemisinins, Trichomes
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