Cited by 241Plant biochemistry and biosynthesisPlant tissue culture and regenerationSesquiterpenes and Asteraceae Studies

Localization of enzymes of artemisinin biosynthesis to the apical cells of glandular secretory trichomes of Artemisia annua L.

Olsson, Mikael E., Olofsson, Linda M., Lindahl, Ann-Louise, Lundgren, Anneli, Brodelius, Maria, Brodelius, Peter E.·2009·Phytochemistry

Abstract

A method based on the laser microdissection pressure catapulting technique has been developed for isolation of whole intact cells. Using a modified tissue preparation method, one outer pair of apical cells and two pairs of sub-apical, chloroplast-containing cells, were isolated from glandular secretory trichomes of Artemisia annua. A. annua is the source of the widely used antimalarial drug artemisinin. The biosynthesis of artemisinin has been proposed to be located to the glandular trichomes. The first committed steps in the conversion of FPP to artemisinin are conducted by amorpha-4,11-diene synthase, amorpha-4,11-diene hydroxylase, a cytochrome P450 monooxygenase (CYP71AV1) and artemisinic aldehyde Delta11(13) reductase. The expression of the three biosynthetic enzymes in the different cell types has been studied. In addition, the expression of farnesyldiphosphate synthase producing the precursor of artemisinin has been investigated. Our experiments showed expression of farnesyldiphosphate synthase in apical and sub-apical cells as well as in mesophyl cells while the three enzymes involved in artemisinin biosynthesis were expressed only in the apical cells. Elongation factor 1alpha was used as control and it was expressed in all cell types. We conclude that artemisinin biosynthesis is taking place in the two outer apical cells while the two pairs of chloroplast-containing cells have other functions in the overall metabolism of glandular trichomes.

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Crossref · 10.1016/j.phytochem.2009.07.009

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Authors
Olsson, M. E., Olofsson, L. M., Lindahl, A.-L., Lundgren, A., Brodelius, M., & Brodelius, P. E.
Corresponding author
  • Brodelius, Maria
  • Brodelius, Peter E.
Venue
Phytochemistry
Publisher
Elsevier BV
Published
2009-06
Volume
70
Issue
9
Pages
1123-1128
ISSN
0031-9422 (ISSN-L)
OpenAlex
W1967648518
Cited by
241
References
27
Citations by year
2026: 15 · 2025: 7 · 2024: 14 · 2023: 12 · 2022: 15 · 2021: 14 · 2020: 19 · 2019: 17 · 2018: 8 · 2017: 10 · 2016: 17 · 2015: 15 · 2014: 10 · 2013: 12 · 2012: 19
FWCI
7.51
Type
journal article
Language
en
Open access
closed
Locations
  • Phytochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
Crafoordska Stiftelsen
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
Keywords
artemisinin biosynthesis, Artemisia annua, apical cells, amorpha-4,11-diene synthase, CYP71AV1, enzyme localization, laser microdissection, gene expression
MeSH terms
Base Sequence, Chloroplasts, Cytochrome P-450 Enzyme System, Gas Chromatography-Mass Spectrometry, Oxidoreductases, Plant Proteins, Plant Leaves, Alkyl and Aryl Transferases, Artemisia annua, Artemisinins

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