The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Engineering canker‐resistant plants through CRISPR /Cas9‐targeted editing of the susceptibility gene Cs LOB 1 promoter in citrus
Open accessAbstract
Summary: Citrus canker, caused by Xanthomonas citri subsp. citri ( Xcc ), is severely damaging to the global citrus industry. Targeted editing of host disease‐susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through CRISPR /Cas9‐targeted modification of the susceptibility gene Cs LOB 1 promoter in citrus. Wanjincheng orange ( Citrus sinensis Osbeck) harbours at least three copies of the Cs LOB 1 G allele and one copy of the Cs LOB 1 − allele. The promoter of both alleles contains the effector binding element ( EBE P thA4 ), which is recognized by the main effector PthA4 of Xcc to activate Cs LOB 1 expression to promote citrus canker development. Five pC as9/Cs LOB 1sg RNA constructs were designed to modify the EBE P thA4 of the Cs LOB 1 promoter in Wanjincheng orange. Among these constructs, mutation rates were 11.5%–64.7%. Homozygous mutants were generated directly from citrus explants. Sixteen lines that harboured EBE P thA4 modifications were identified from 38 mutant plants. Four mutation lines (S2‐5, S2‐6, S2‐12 and S5‐13), in which promoter editing disrupted Cs LOB 1 induction in response to Xcc infection, showed enhanced resistance to citrus canker compared with the wild type. No canker symptoms were observed in the S2‐6 and S5‐13 lines. Promoter editing of Cs LOB 1 G alone was sufficient to enhance citrus canker resistance in Wanjincheng orange. Deletion of the entire EBE P thA4 sequence from both Cs LOB 1 alleles conferred a high degree of resistance to citrus canker. The results demonstrate that CRISPR /Cas9‐mediated promoter editing of Cs LOB 1 is an efficient strategy for generation of canker‐resistant citrus cultivars.
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Applications of CRISPR–Cas in agriculture and plant biotechnology
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CRISPR for Crop Improvement: An Update Review
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Xanthomonas diversity, virulence and plant–pathogen interactions
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CRISPR/Cas: A powerful tool for gene function study and crop improvement
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Metadata
- Authors
- Peng, A., Chen, S., Lei, T., Xu, L., He, Y., Wu, L., Yao, L., & Zou, X.
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2017-05-03
- Volume
- 15
- Issue
- 12
- Pages
- 1509-1519
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W2598396806
- Cited by
- 731
- References
- 36
- FWCI
- 50.56
- Type
- journal article
- Language
- en
- Open access
- gold
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