CRISPR/Cas system: recent advances and future prospects for genome editing

Manghwar, Hakim; Lindsey, Keith; Zhang, Xianlong; Jin, Shuangxia

doi:10.1016/j.tplants.2019.09.006

Cited by 529CRISPR and Genetic EngineeringGenetically Modified Organisms ResearchRNA regulation and disease

CRISPR/Cas system: recent advances and future prospects for genome editing

Open access

Manghwar, Hakim, Lindsey, Keith, Zhang, Xianlong, Jin, Shuangxia·2019·Trends in Plant Science

DOI 10.1016/j.tplants.2019.09.006

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Abstract

Genome editing (GE) has revolutionized biological research through the new ability to precisely edit the genomes of living organisms. In recent years, various GE tools have been explored for editing simple and complex genomes. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has widely been used in GE due to its high efficiency, ease of use, and accuracy. It can be used to add desirable and remove undesirable alleles simultaneously in a single event. Here, we discuss various applications of CRISPR/Cas9 in a range of important crops, compare it with other GE tools, and review its mechanism, limitations, and future possibilities. Various newly emerging CRISPR/Cas systems, including base editing (BE), xCas9, and Cas12a (Cpf1), are also considered.

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Method
Structure and Engineering of Francisella novicida Cas9
Hirano, H., Gootenberg, J. S., Horii, T., Abudayyeh, O. O., Kimura, M., Hsu, P. D., Nakane, T., Ishitani, R., Hatada, I., Zhang, F., et al. (2016) · Cell · doi:10.1016/j.cell.2016.01.039 · Cited by 361 · cited 1× · see citing works
“structural information for creating a variant which can identify more relaxed 5ʹ-YG-3ʹ PAM [105]”
Background
Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes
Wang, C., Liu, Q., Shen, Y. X., Hua, Y., Wang, J., Lin, J., Wu, M., Sun, T., Cheng, Z., Mercier, R., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-018-0003-0 · Cited by 357 · cited 2× · see citing works
“It can be achieved in two ways, by either constructing multiple gRNA expression cassettes in separate vectors or assembling various sgRNAs in a single vector [82].”
Background
An Agrobacterium‐delivered CRISPR /Cas9 system for high‐frequency targeted mutagenesis in maize
Char, S. N., Neelakandan, A. K., Nahampun, H. N., Frame, B. R., Main, M., Spalding, M. H., Becraft, P. W., Meyers, B. C., Walbot, V., Wang, K., et al. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12611 · Cited by 352 · cited 1× · see citing works
“The NHEJ frequently leads to random DNA indels at the cleavage position, whereas HDR executes the precise sequence insertion or gene replacement by adding a donor DNA template with sequence homology at the predicted DSB site [100].”
Background
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
Kocak, D. D., Josephs, E. A., Bhandarkar, V., Adkar, S. S., Kwon, J. B., & Gersbach, C. A. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0095-1 · Cited by 351 · cited 1× · see citing works
“A study by Kocak and coworkers [40] showed that the engineering of a hairpin secondary structure onto the spacer region of sgRNAs (hp-sgRNAs) can increase the specificity by several orders of magnitude when combined with numerous CRISPR effectors.”
Background
Auxin binding protein 1 (ABP1) is not required for either auxin signaling or Arabidopsis development
Gao, Y., Zhang, Y., Zhang, D., Dai, X., Estelle, M. A., & Zhao, Y. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1500365112 · Cited by 341 · cited 1× · see citing works
“plays a role in strigolactone transport [129]”
Design of a bacterial speck resistant tomato by CRISPR /Cas9‐mediated editing of Sl JAZ 2
Ortigosa, A., Ibanez, S. G., Leonhardt, N., & Solano, R. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13006 · Cited by 339 · cited 1× · see citing works
“) [63], potato (Solanum tuberosum) [64], sweet orange (Citrus sinensis L.”
Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
Zischewski, J., Fischer, R., & Bortesi, L. (2017) · Biotechnology Advances · doi:10.1016/j.biotechadv.2016.12.003 · Cited by 323 · cited 2× · see citing works
“Further sequencing and gel electrophoresis might be used to analyze amplicons [48].”
BackgroundMethod
Towards CRISPR /Cas crops – bringing together genomics and genome editing
Scheben, A., Wolter, F., Batley, J., Puchta, H., & Edwards, D. (2017) · New Phytologist · doi:10.1111/nph.14702 · Cited by 319 · cited 1× · see citing works
“As described above, the CRISPR/Cas system can induce sequence-specific mutagenesis to interrupt genes to evaluate their functions and be used for trait improvement in crops [77].”
Background
Modification of the PthA4 effector binding elements in Type I Cs LOB 1 promoter using Cas9/sg RNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCs LOB 1.3 infection
Jia, H., Orbović, V., Jones, J. B., & Wang, N. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12495 · Cited by 310 · see citing works
CRISPR/Cas9‐mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean
Cai, Y., Chen, L., Liu, X., Guo, C., Sun, S., Wu, C., Jiang, B., Han, T., & Hou, W. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12758 · Cited by 309 · see citing works
Mutation detection using Surveyor™ nuclease
Qiu, P., Shandilya, H., D'Alessio, J. M., O’Connor, K. C., Durocher, J. A., & Gerard, G. F. (2004) · BioTechniques · doi:10.2144/04364pf01 · Cited by 299 · see citing works
Applying CRISPR/Cas for genome engineering in plants: the best is yet to come
Puchta, H. (2016) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2016.11.011 · Cited by 298 · cited 2× · see citing works
“The tracrRNA binds to the crRNA and Cas9 protein that is recruited to the complex [2].”
Background
High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system
Wang, P., Zhang, J., Sun, L., Ma, Y., Xu, J., Liang, S., Deng, J., Tan, J., Zhang, Q., Tu, L., et al. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12755 · Cited by 294 · cited 1× · see citing works
“reporter due to its different benefits over other report proteins [117]”
Background
Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain
Gallego‐Bartolomé, J., Gardiner, J., Liu, W., Papikian, A., Ghoshal, B., Kuo, H. Y., Zhao, J. M.-C., Segal, D. J., & Jacobsen, S. E. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1716945115 · Cited by 290 · cited 7× · see citing works
“CRISPR/Cas9 technology has been used to modify a wide range of plant species, includingArabidopsis [53], rice [54], wheat (Triticum aestivum) [55], maize [56], soybean (Glycine max) [57], sorghum [58], cotton (Gossypium hirsutum L.) [52,59], rapeseed (Brassica napus L.) [60], barley (Hordeum vulgare L.) [61], Nicotian…”
BackgroundInfluential
CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. Rasthali genome
Kaur, N., Alok, A., Shivani, Kaur, N., Pandey, P., Awasthi, P., & Tiwari, S. (2017) · Functional & Integrative Genomics · doi:10.1007/s10142-017-0577-5 · Cited by 286 · see citing works
Efficient CRISPR/Cas9-based gene knockout in watermelon
Tian, S., Jiang, L., Gao, Q., Zhang, J., Zong, M., Zhang, H., Ren, Y., Guo, S., Gong, G., Liu, F., et al. (2016) · Plant Cell Reports · doi:10.1007/s00299-016-2089-5 · Cited by 278 · cited 2× · see citing works
“Guided by the sgRNA, the sgRNA and Cas9 search for the target across the genome and create blunt-ended DSBs at about 3 bp upstream of the PAM site (Figure 1A) [76].”
Background
Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9
Ueta, R., Abe, C., Watanabe, T., Sugano, S. S., Ishihara, R., Ezura, H., Osakabe, Y., & Osakabe, K. (2017) · Scientific Reports · doi:10.1038/s41598-017-00501-4 · Cited by 278 · see citing works
A potent Cas9-derived gene activator for plant and mammalian cells
Li, Z., Zhang, D., Xiong, X., Yan, B., Xie, W., Sheen, J., & Li, J. (2017) · Nature Plants · doi:10.1038/s41477-017-0046-0 · Cited by 278 · cited 1× · see citing works
“This assembly promoted up to 55-fold activation of the target gene compared with the conventional dCas9-VP64 system [89].”
Background
CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion
Ali, Z., Ali, S., Tashkandi, M., Zaidi, S. S., & Mahfouz, M. M. (2016) · Scientific Reports · doi:10.1038/srep26912 · Cited by 274 · cited 1× · see citing works
“CRISPR/Cas14a is a potential system for engineering resistance against plant ssDNA viruses belonging to the Geminiviridae and Nanoviridae families [29].”
Background
CRISPR-Cas9 Targeted Mutagenesis Leads to Simultaneous Modification of Different Homoeologous Gene Copies in Polyploid Oilseed Rape (Brassica napus)
Braatz, J., Harloff, H., Mascher, M., Stein, N., Himmelbach, A., & Jung, C. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00426 · Cited by 265 · see citing works
CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.
Sugano, S. S., Shirakawa, M., Takagi, J., Matsuda, Y., Shimada, T., Hara‐Nishimura, I., & Kohchi, T. (2014) · Plant and Cell Physiology · doi:10.1093/pcp/pcu014 · Cited by 261 · cited 1× · see citing works
“) [68], Medicago truncatula [69],Marchantia polymorpha [70], tobacco (Nicotiana tabacum L.”
Background
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells
Lee, C. M., Cradick, T. J., & Bao, G. (2016) · Molecular Therapy · doi:10.1038/mt.2016.8 · Cited by 244 · cited 1× · see citing works
“NmCas9 can use an sgRNA to direct its activity [107]”
Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System
Zhou, H. L., He, M., Li, J., Chen, L., Huang, Z., Zheng, S., Zhu, L., Ni, E., Jiang, D., Zhao, B., et al. (2016) · Scientific Reports · doi:10.1038/srep37395 · Cited by 244 · see citing works
Site-directed mutagenesis in Petunia × hybrida protoplast system using direct delivery of purified recombinant Cas9 ribonucleoproteins
Subburaj, S., Chung, S. J., Lee, C., Ryu, S.-M., Kim, D. H., Kim, J.-S., Bae, S., & Lee, G.-J. (2016) · Plant Cell Reports · doi:10.1007/s00299-016-1937-7 · Cited by 237 · see citing works
Efficient CRISPR/Cas9 Genome Editing of Phytoene desaturase in Cassava
Odipio, J., Alicai, T., Ingelbrecht, I., Nusinow, D. A., Bart, R. S., & Taylor, N. J. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01780 · Cited by 235 · cited 2× · see citing works
“the carotenoid biosynthesis pathway [75]”
Activities and specificities of CRISPR /Cas9 and Cas12a nucleases for targeted mutagenesis in maize
Lee, K., Zhang, Y., Kleinstiver, B. P., Guo, J. A., Aryee, M. J., Miller, J., Malzahn, A. A., Zarecor, S., Lawrence‐Dill, C. J., Joung, J. K., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12982 · Cited by 235 · cited 1× · see citing works
“CRISPR/Cas9 technology has been used to modify a wide range of plant species, includingArabidopsis [53], rice [54], wheat (Triticum aestivum) [55], maize [56], soybean (Glycine max) [57], sorghum [58], cotton (Gossypium hirsutum L.”
Background
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes
Hess, G. T., Tycko, J., Yao, D., & Bassik, M. C. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.029 · Cited by 234 · cited 14× · see citing works
“NHEJ-mediated repair can be used to generate point mutations via gene replacement when the target sequences of CRISPR/Cas9 are located in introns.”
BackgroundMethodInfluential
Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems
Lowder, L. G., Zhou, J., Zhang, Y., Malzahn, A., Zhong, Z., Hsieh, T.-F., Voytas, D. F., Zhang, Y., & Qi, Y. (2018) · Molecular Plant · doi:10.1016/j.molp.2017.11.010 · Cited by 232 · cited 1× · see citing works
“These tools can activate more than four genes at the same time and can be used to evaluate positive feedback transcriptional loops and the control of tissue-specific gene activation [87]; however, it does introduce more off-target effects [88].”
Background
Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
Jeon, Y., Choi, Y. H., Jang, Y., Yu, J., Goo, J., Lee, G., Jeong, Y. K., Lee, S. H., Kim, I.-S., Kim, J., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05245-x · Cited by 229 · cited 3× · see citing works
“However, it lacks the His-Asn-His (HNH) domain and it may include a single active site in the RuvC domain [21].”
BackgroundMethodInfluential
Application of CRISPR/Cas9 in plant biology
Liu, X., Wu, S., Xu, J., Sui, C., & Wei, J. (2017) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2017.01.002 · Cited by 226 · cited 1× · see citing works
“The HNH domain cuts a complementary strand of crRNA, whereas the RuvC-like domain cuts an opposite strand of dsDNA [101].”
Background
Editing of an Alpha-Kafirin Gene Family Increases, Digestibility and Protein Quality in Sorghum
Li, A., Jia, S., Yobi, A., Ge, Z., Sato, S. J., Zhang, C., Angelovici, R., Clemente, T. E., & Holding, D. R. (2018) · PLANT PHYSIOLOGY · doi:10.1104/pp.18.00200 · Cited by 215 · see citing works
CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme
Michno, J., Wang, X., Liu, J., Curtin, S. J., Kono, T. J. Y., & Stupar, R. M. (2015) · GM crops & food · doi:10.1080/21645698.2015.1106063 · Cited by 208 · cited 1× · see citing works
“) [68], Medicago truncatula [69],Marchantia polymorpha [70], tobacco (Nicotiana tabacum L.”
Background
Whole genome sequencing reveals rare off‐target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR /Cas9‐edited cotton plants
Li, J., Manghwar, H., Sun, L., Wang, P., Wang, G., Sheng, H., Zhang, J., Liu, H., Qin, L., Rui, H., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13020 · Cited by 205 · see citing works
Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine
Pessina, S., Lenzi, L., Perazzolli, M., Campa, M., Costa, L. D., Urso, S., Valè, G., Salamini, F., Velasco, R., & Malnoy, M. (2016) · Horticulture Research · doi:10.1038/hortres.2016.16 · Cited by 200 · see citing works
CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus
Yang, H., Wu, J.-J., Tang, T., Liu, K.-D., & Dai, C. (2017) · Scientific Reports · doi:10.1038/s41598-017-07871-9 · Cited by 199 · cited 1× · see citing works
“are serving as negative regulators of organ size [122]”
Background
CRISPR /Cas9‐mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β‐1,2‐xylose and core α‐1,3‐fucose
Jansing, J., Sack, M., Augustine, S. M., Fischer, R., & Bortesi, L. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12981 · Cited by 199 · see citing works
Epigenetic Modifications and Plant Hormone Action
Yamamuro, C., Zhu, J., & Yang, Z. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.10.008 · Cited by 197 · cited 1× · see citing works
“Epigenetic and post-translational protein modifications, for example, DNA and histone acetylation/ methylation, ubiquitination, SUMOylation, and phosphorylation, can alter chromatin structure and regulate gene expression patterns [90].”
Background
CRISPR Cpf1 proteins: structure, function and implications for genome editing
Safari, F., Zare, K., Negahdaripour, M., Barekati‐Mowahed, M., & Ghasemi, Y. (2019) · Cell & Bioscience · doi:10.1186/s13578-019-0298-7 · Cited by 196 · see citing works
Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza
Li, B., Cui, G., Shen, G., Zhan, Z.-L., Huang, L., Chen, J., & Qi, X. (2017) · Scientific Reports · doi:10.1038/srep43320 · Cited by 191 · see citing works
Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM
Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 185 · cited 1× · see citing works
“SpCas9-NG has been used for gene disruption and cytosine BE in rice [15].”
Method
C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism
Liu, L., Chen, P., Wang, M., Li, X., Wang, J., Yin, M., & Wang, Y. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.11.040 · Cited by 182 · cited 1× · see citing works
“with a bi-lobed architecture that consists of an REC and NUC lobe [111] Table 1.”
Background
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
Moon, S. B., Lee, J. M., Kang, J. G., Lee, N.-E., Ha, D.-I., Kim, D. Y., Kim, S. H., Yoo, K., Kim, J., Kim, J., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-06129-w · Cited by 182 · see citing works
CRISPR /Cas9‐mediated knockout of Ms1 enables the rapid generation of male‐sterile hexaploid wheat lines for use in hybrid seed production
Okada, A., Arndell, T., Borisjuk, N. V., Sharma, N., Watson‐Haigh, N. S., Tucker, E. J., Baumann, U., Langridge, P., & Whitford, R. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13106 · Cited by 181 · cited 1× · see citing works
“CRISPR/Cas9 technology has been used to modify a wide range of plant species, includingArabidopsis [53], rice [54], wheat (Triticum aestivum) [55], maize [56], soybean (Glycine max) [57], sorghum [58], cotton (Gossypium hirsutum L.”
Background
High‐efficient and precise base editing of C•G to T•A in the allotetraploid cotton (Gossypium hirsutum) genome using a modified CRISPR /Cas9 system
Qin, L., Li, J., Wang, Q., Xu, Z., Sun, L., Alariqi, M., Manghwar, H., Wang, G., Li, B., Ding, X. L., et al. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13168 · Cited by 179 · see citing works
Applications of CRISPR/Cas System to Bacterial Metabolic Engineering
Cho, S., Shin, J., & Cho, B. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19041089 · Cited by 172 · cited 1× · see citing works
“In bacterial cells, CRISPRi is a more useful genetic engineering tool for gene knockdown compared with RNAi, because it avoids cell death through disruption of the genome [85].”
Background
Generation of chromosomal deletions in dicotyledonous plants employing a user‐friendly genome editing toolkit
Ordon, J., Gantner, J., Kemna, J., Lennart, S., Reschke, M., Streubel, J., Boch, J., & Stuttmann, J. (2016) · The Plant Journal · doi:10.1111/tpj.13319 · Cited by 167 · cited 1× · see citing works
“regulators of plant innate immunity [131]”
CRISPR/Cas9-mediated genome editing and gene replacement in plants: Transitioning from lab to field
Schaeffer, S. M., & Nakata, P. A. (2015) · Plant Science · doi:10.1016/j.plantsci.2015.09.011 · Cited by 162 · cited 1× · see citing works
“CRISPR/Cas has successfully been used for gene replacement in plants [94].”
Method
A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)
Li, C., Ünver, T., & Zhang, B. (2017) · Scientific Reports · doi:10.1038/srep43902 · Cited by 158 · cited 1× · see citing works
“involved in the development of cotton fiber [118]”
Background
CRISPR-Cpf1: A New Tool for Plant Genome Editing
Zaidi, S. S.-E.-A., Mahfouz, M. M., & Mansoor, S. (2017) · Trends in Plant Science · doi:10.1016/j.tplants.2017.05.001 · Cited by 155 · cited 1× · see citing works
“However, Cpf1mediated GE is cheaper than SpCas9 because it requires only short synthetic single-guide RNA (sgRNA) sequences [19].”
Background
Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia
Zhang, B., Yang, X., Yang, C., Li, M., & Guo, Y. (2016) · Scientific Reports · doi:10.1038/srep20315 · Cited by 153 · cited 1× · see citing works
“the biosynthesis of carotenoid and chlorophyll [132]”
A Streamlined CRISPR Pipeline to Reliably Generate Zebrafish Frameshifting Alleles
Talbot, J. C., & Amacher, S. L. (2014) · Zebrafish · doi:10.1089/zeb.2014.1047 · Cited by 152 · see citing works
Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system
Chen, X., Lu, X., Shu, N., Wang, S., Wang, J., Wang, D., Guo, L., & Ye, W. (2017) · Scientific Reports · doi:10.1038/srep44304 · Cited by 152 · cited 1× · see citing works
“method Mutations were detected in cotton protoplast – [116]”
Method
Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice
Ren, B., Liu, L., Li, S., Kuang, Y., Wang, J., Zhang, D., Zhou, X., Lin, H., & Zhou, H. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.010 · Cited by 146 · cited 2× · see citing works
“Recently, Ren and coworkers [14] evaluated the nuclease activity of Cas9-NG towards various NGN PAMs by Box 1.”
Background
Genome Engineering in Rice Using Cas9 Variants that Recognize NG PAM Sequences
Hua, K., Tao, X., Han, P., Wang, R., & Zhu, J. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.009 · Cited by 140 · see citing works
Developing a flexible, high‐efficiency Agrobacterium‐mediated sorghum transformation system with broad application
Che, P., Anand, A., Wu, E., Sander, J. D., Simon, M. K., Zhu, W., Sigmund, A. L., Zastrow‐Hayes, G., Miller, M., Liu, D., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12879 · Cited by 138 · see citing works
Inactivation of the β(1,2)-xylosyltransferase and the α(1,3)-fucosyltransferase genes in Nicotiana tabacum BY-2 Cells by a Multiplex CRISPR/Cas9 Strategy Results in Glycoproteins without Plant-Specific Glycans
Mercx, S., Smargiasso, N., Chaumont, F., Pauw, E. D., Boutry, M., & Navarre, C. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00403 · Cited by 137 · see citing works
Live‐cell CRISPR imaging in plants reveals dynamic telomere movements
Dreißig, S., Schiml, S., Schindele, P., Weiß, O., Rutten, T. L. M., Schubert, V., Gladilin, E., Mette, M. F., Puchta, H., & Houben, A. (2017) · The Plant Journal · doi:10.1111/tpj.13601 · Cited by 134 · see citing works
Overcoming Self-Incompatibility in Diploid Potato Using CRISPR-Cas9
Enciso-Rodríguez, F. E., Manrique-Carpintero, N. C., Nadakuduti, S. S., Buell, C. R., Zarka, D. G., & Douches, D. S. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00376 · Cited by 134 · see citing works
Efficient Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus japonicus Using CRISPR-Cas9
Wang, L., Wang, L., Tan, Q. W., Fan, Q., Zhu, H., Hong, Z., Zhang, Z., & Duanmu, D. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01333 · Cited by 126 · see citing works
The Current State and Future of CRISPR-Cas9 gRNA Design Tools
Wilson, L. O. W., O’Brien, A. R., & Bauer, D. C. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.00749 · Cited by 124 · see citing works
The CRISPR /Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists
Wolter, F., & Puchta, H. (2018) · The Plant Journal · doi:10.1111/tpj.13899 · Cited by 112 · cited 2× · see citing works
“Due to the presence of higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domains, it is associated with RNase activity [5].”
Background
Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
Mir, A., Alterman, J. F., Hassler, M. R., Debacker, A. J., Hudgens, E. D., Echeverria, D., Brodsky, M. H., Khvorova, A., Watts, J. K., & Sontheimer, E. J. (2018) · Nature Communications · doi:10.1038/s41467-018-05073-z · Cited by 112 · see citing works
Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation
Braun, C. J., Bruno, P. M., Horlbeck, M. A., Gilbert, L. A., Weissman, J. S., & Hemann, M. T. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1600582113 · Cited by 101 · see citing works
xC as9 expands the scope of genome editing with reduced efficiency in rice
Wang, J., Meng, X., Hu, X., Sun, T., Li, J., Wang, K., & Yu, H. J. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13053 · Cited by 87 · cited 4× · see citing works
“Phage-assisted continuous evolution was used to develop an SpCas9 variant, xCas9(3.7), which recognizes a broader range of protospacer adjacent motifs (PAMs) [10]. xCas9 possesses a higher DNA specificity and editing efficiency, lower off-target activity, and broader PAM compatibility (including NG, GAA, and GAT) than…”
BackgroundInfluential
cis–trans Engineering: Advances and Perspectives on Customized Transcriptional Regulation in Plants
Shrestha, A., Khan, A., & Dey, N. (2018) · Molecular Plant · doi:10.1016/j.molp.2018.05.008 · Cited by 86 · cited 2× · see citing works
“dCas9 fused to epigenetic regulatory factors involved in histone acetylation, or methylation of DNA, can be used to modulate chromatin activity and gene expression patterns involved in plant development and environmental adaptation [86].”
Background
Molecular architectures and mechanisms of Class 2 CRISPR-associated nucleases
Garcia‐Doval, C., & Jínek, M. (2017) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2017.10.015 · Cited by 85 · see citing works
Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA
Kanazashi, Y., Hirose, A., Takahashi, I., Mikami, M., Endo, M., Hirose, S., Toki, S., Kaga, A., Naito, K., Ishimoto, ‍., et al. (2018) · Plant Cell Reports · doi:10.1007/s00299-018-2251-3 · Cited by 83 · see citing works
An Efficient CRISPR/Cas9 Platform for Rapidly Generating Simultaneous Mutagenesis of Multiple Gene Homoeologs in Allotetraploid Oilseed Rape
Li, C., Hao, M., Wang, W., Wang, H., Chen, F., Chu, W., Zhang, B., Mei, D., Cheng, H., & Hu, Q. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00442 · Cited by 74 · see citing works
Targeting Plant ssDNA Viruses with Engineered Miniature CRISPR-Cas14a
Khan, M. Z., Haider, S., Mansoor, S., & Amin, I. U. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.03.015 · Cited by 73 · cited 3× · see citing works
“It has been shown by in vitro validation of the PAM requirement that Cas14a can cleave target sites irrespective of the different sequences adjacent to the targets of these different guides [27].”
BackgroundInfluential
Repair of adjacent single-strand breaks is often accompanied by the formation of tandem sequence duplications in plant genomes
Schiml, S., Fauser, F., & Puchta, H. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1603823113 · Cited by 69 · see citing works
APOBEC: From mutator to editor
Yang, B., Li, X., Lei, L., & Chen, J. (2017) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2017.04.009 · Cited by 67 · cited 2× · see citing works
“BE technology has been widely used in various organisms and cell lines [30,31].”
BackgroundMethod
The Conspicuity of CRISPR-Cpf1 System as a Significant Breakthrough in Genome Editing
Bayat, H., Modarressi, M. H., & Rahimpour, A. (2017) · Current Microbiology · doi:10.1007/s00284-017-1406-8 · Cited by 56 · see citing works
Heritability of targeted gene modifications induced by plant-optimized CRISPR systems
Mao, Y., Botella, J. R., & Zhu, J. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2380-1 · Cited by 55 · cited 1× · see citing works
“The PAM is a 3-nt (NGG) sequence located immediately downstream of the single-guide RNA (sgRNA) target site, which plays an essential role in binding and for Cas9-mediated DNA cleavage [103].”
Background
High‐throughput detection and screening of plants modified by gene editing using quantitative real‐time polymerase chain reaction
Peng, C., Wang, H., Xu, X., Wang, X., Chen, X., Wei, W., Lai, Y., Liu, G., Godwin, I. D., Li, J., et al. (2018) · The Plant Journal · doi:10.1111/tpj.13961 · Cited by 53 · cited 1× · see citing works
“It is an efficient, simple, and rapid method to detect induced mutations [43].”
Method
Verification of DNA motifs in Arabidopsis using CRISPR /Cas9‐mediated mutagenesis
Li, C., Chen, C., Chen, H., Wang, S., Chen, X., & Cui, Y. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.12886 · Cited by 25 · cited 1× · see citing works
“CRISPR/Cas9 technology has been used to modify a wide range of plant species, includingArabidopsis [53], rice [54], wheat (Triticum aestivum) [55], maize [56], soybean (Glycine max) [57], sorghum [58], cotton (Gossypium hirsutum L.”
Background
Structural Recognition between a Four-way DNA Junction and a Resolving Enzyme
Déclais, A., Liu, J., Freeman, A. D. J., & Lilley, D. M. J. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.04.037 · Cited by 22 · cited 1× · see citing works
“T7E1 can, however, recognize and cleave various dsDNA molecules if their structure is curved and able to bend further [46].”
Background
Research of methods to detect genomic mutations induced by CRISPR/Cas systems
Wang, K., Mei, D. Y., Liu, Q. N., Qiao, X. H., Ruan, W., Huang, T., & Cao, G. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.09.029 · Cited by 22 · see citing works

Flagged first, then by citations

4 of the 140 references couldn’t be matched to a known paper.

Sorted by citations

CRISPR/Cas9 therapeutics: progress and prospects
Li, T., Yang, Y., Qi, H., Cui, W., Zhang, L., Fu, X., He, X., Liu, M., Li, P., & Yu, T. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01309-7 · Cited by 770 · see citing works
“INTRODUCTION Gene editing is a technology that precisely modifies the genome sequence to induce insertions, deletions, or base substitutions in the genome.(1,2) Many diseases are accompanied by changes in gene expression in vivo, particularly some genetic diseases caused by mutations in a single gene, and gene-editing…”
Background
Plant organellar genomes: much done, much more to do
Wang, J., Kan, S., Liao, X., Zhou, J., Tembrock, L. R., Daniell, H., Jin, S., & Wu, Z. (2024) · Trends in Plant Science · doi:10.1016/j.tplants.2023.12.014 · Cited by 288 · see citing works
Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root
Zhang, T., Chen, Y., Liu, Y., Lin, W., & Wang, J. (2021) · Nature Communications · doi:10.1038/s41467-021-22352-4 · Cited by 273 · see citing works
Comprehensive review of CRISPR-based gene editing: Mechanisms, challenges, and applications in cancer therapy
Chehelgerdi, M., Chehelgerdi, M., Khorramian-Ghahfarokhi, M., Shafieizadeh, M., Mahmoudi, E., Eskandari, F., Rashidi, M., Arshi, A., & Mokhtari-Farsani, A. (2024) · Molecular Cancer · doi:10.1186/s12943-023-01925-5 · Cited by 251 · see citing works
“Targeting these genes with drugs may disrupt critical cellular processes, leading to the selective killing or suppression of cancer cells while minimizing harm to normal cells [329, 331, 337, 338].”
Background
Genome editing with the CRISPR‐Cas system: an art, ethics and global regulatory perspective
Zhang, D., Hussain, A., Manghwar, H., Xie, K., Xie, S., Zhao, S., Larkin, R. M., Qing, P., Jin, S., & Ding, F. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13383 · Cited by 245 · see citing works
“Comparing with DSB-HDR-mediated genome editing, base editing systems comprise site-directed alteration of DNA base pairs along with manipulating a DNA repair mechanism to achieve a precise repairing of edited base (Hess et al., 2017; Manghwar et al., 2019).”
Method
Phytoremediation as a green biotechnology tool for emerging environmental pollution: A step forward towards sustainable rehabilitation of the environment
Kurade, M. B., Ha, Y., Xiong, J.-Q., Govindwar, S. P., Jang, M., & Jeon, B. (2021) · Chemical Engineering Journal · doi:10.1016/j.cej.2021.129040 · Cited by 235 · see citing works
Improving nutritional quality of rice for human health
Zhao, M., Lin, Y., & Chen, H. (2020) · Theoretical and Applied Genetics · doi:10.1007/s00122-019-03530-x · Cited by 224 · see citing works
“However, target induced mutagenesis technologies such as genome editing especially that based on CRISPR/Cas systems are available to create site-targeted mutations (Manghwar et al. 2019).”
Background
Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Yang, L., Machin, F. Q., Wang, S., Saplaoura, E., & Kragler, F. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01585-8 · Cited by 221 · see citing works
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
Sun, C., Yuan, L., Li, B., Gao, Q., Li, Y., Cao, W., Yang, C., Li, H., Wang, Z., Li, Y., et al. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01769-w · Cited by 206 · see citing works
“Thus, despite the rapid expansion of the genome editing toolbox 40–42 , it is important to be able to generate precise (defined as predictable and as specified) large DNA insertions in plants.”
Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement
Ahmar, S., Mahmood, T., Fiaz, S., Mora, F., Shafique, M. S., Chattha, M. S., & Jung, K.-H. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.663849 · Cited by 186 · see citing works
Control of Plant Growth and Defense by Photoreceptors: From Mechanisms to Opportunities in Agriculture
Pierik, R., & Ballaré, C. L. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.11.021 · Cited by 164 · see citing works
Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing
Deng, F., Li, Y., Yang, B., Sang, R., Deng, W., Kansara, M., Lin, F. P. Y., Thavaneswaran, S., Thomas, D. M., & Goldys, E. M. (2024) · Nature Communications · doi:10.1038/s41467-024-46001-8 · Cited by 160 · see citing works
“Short ssDNA-linked ﬂ uorescent quenched reporters 31 monitored the overall reporter trans -cleavage rate (Method 7).”
BackgroundMethod
Molecular mechanisms of plant tolerance to heat stress: current landscape and future perspectives
Haider, S., Iqbal, J., Naseer, S., Yaseen, T., Shaukat, M., Bibi, H., Ahmad, Y., Daud, H., Abbasi, N. L., & Mahmood, T. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02696-3 · Cited by 156 · see citing works
“CRISPR has revolutionized plant biology and CRISPR/Cas9 and associated systems have been used to modify genes to increase crop productivity, enhance grain quality, increase biotic and abiotic stress tolerance (Manghwar et al. 2019).”
Background
A comprehensive overview of cotton genomics, biotechnology and molecular biological studies
Wen, X., Chen, Z., Yang, Z., Wang, M., Jin, S., Wang, G., Zhang, L., Wang, L.-J., Li, J., Saeed, S., et al. (2023) · Science China Life Sciences · doi:10.1007/s11427-022-2278-0 · Cited by 152 · see citing works
“Currently, the best studied and most frequently applied system is CRISPR/Cas9 belonging to the type II of class 2 system, which consists of two main constituents: a single guide RNA (sgRNA) that recognizes the DNA binding site and Cas9 induces DSBs at the target site (Manghwar et al., 2019).”
Background
Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
Wu, Y., Battalapalli, D., Hakeem, M. J., Selamneni, V., Zhang, P., Draz, M. S., & Ruan, Z. (2021) · Journal of Nanobiotechnology · doi:10.1186/s12951-021-01132-8 · Cited by 152 · see citing works
“The CRISPR-Cas system has been rapidly deployed for genome editing in various cell types and experimental setups [12].”
Method
Engineered probiotics
Ma, J., Lyu, Y., Liu, X., Jia, X., Cui, F., Wu, X., Deng, S., & Yue, C. (2022) · Microbial Cell Factories · doi:10.1186/s12934-022-01799-0 · Cited by 153 · see citing works
“Therefore, although ZFNs and TALENs have been used in gene editing of human, animal, and plant cells since 2002 and 2011, these limiting factors have hindered their wide application to a certain extent [35].”
Background
Current progress and challenges in crop genetic transformation
Anjanappa, R. B., & Gruissem, W. (2021) · Journal of Plant Physiology · doi:10.1016/j.jplph.2021.153411 · Cited by 142 · see citing works
Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview
Ahmad, M., Waraich, E. A., Skalický, M., Hussain, S., Zulfiqar, U., Anjum, M. Z., Rahman, M. H. U., Brestič, M., Ratnasekera, D., Lamilla-Tamayo, L., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.767150 · Cited by 138 · see citing works
“CRISPR/Cas9 and CRISPR/Cas12a arbitrated genome editing induces a DSB at the targeted sites (Manghwar et al., 2019), leading to unintentional changes or production of abnormal protein(s) due to random insets or removals in the plants.”
Background
RNA-Targeting CRISPR-Cas systems and their applications
Burmistrz, M., Krakowski, K., & Krawczyk-Balska, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21031122 · Cited by 125 · see citing works
Versatile detection with CRISPR/Cas system from applications to challenges
Wu, H., Chen, X., Zhang, M., Wang, X., Chen, Y., Qian, C., Wu, J. F., & Xu, J. (2020) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2020.116150 · Cited by 130 · see citing works
CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives
Wang, Y., Zafar, N., Ali, Q., Manghwar, H., Wang, G., Yu, L., Ding, X. L., Ding, F., Hong, N., Wang, G., et al. (2022) · Cells · doi:10.3390/cells11233928 · Cited by 135 · see citing works
Enhancing phytoremediation of hazardous metal(loid)s using genome engineering CRISPR–Cas9 technology
Sarma, H., Islam, N. F., Prasad, R., Prasad, M. N. V., Ma, L. Q., & Rinklebe, J. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.125493 · Cited by 116 · see citing works
Mechanistic insights of CRISPR /Cas‐mediated genome editing towards enhancing abiotic stress tolerance in plants
Bhat, M. A., Mir, R. A., Kumar, V., Shah, A. A., Zargar, S. M., Rahman, S., & Jan, A. T. (2021) · Physiologia Plantarum · doi:10.1111/ppl.13359 · Cited by 124 · see citing works
Redundant and specific roles of individual MIR172 genes in plant development
Lian, H., Wang, L., Ma, N., Zhou, C., Han, L., Zhang, T., & Wang, J. (2021) · PLoS Biology · doi:10.1371/journal.pbio.3001044 · Cited by 112 · see citing works
CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges
Bhowmik, P., Konkin, D. J. F., Polowick, P. L., Hodgins, C. L., Subedi, M., Xiang, D., Yu, B., Patterson, N. A., Rajagopalan, N., Babic, V., et al. (2021) · Legume Science · doi:10.1002/leg3.96 · Cited by 113 · see citing works
“broader PAM compatibility and increased length of gRNA have been used recently (Makarova et al., 2015; Manghwar et al., 2019; Puchta, 2017; Van Eck, 2020).”
Method
Using traditional machine learning and deep learning methods for on- and off-target prediction in CRISPR/Cas9: a review
Sherkatghanad, Z., Abdar, M., Charlier, J., & Makarenkov, V. (2023) · Briefings in Bioinformatics · doi:10.1093/bib/bbad131 · Cited by 117 · see citing works
Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants
Ariga, H., Toki, S., & Ishibashi, K. (2020) · Plant and Cell Physiology · doi:10.1093/pcp/pcaa123 · Cited by 110 · see citing works
Integrating speed breeding with artificial intelligence for developing climate-smart crops
Rai, K. K. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-07769-4 · Cited by 108 · see citing works
“Various Cas proteins have been identified in plants displaying sequence-specific nuclease activity to minimize the impediment and increase the specificity of the CRISPR-Cas system for their effective exploitation [ 50 ].”
BackgroundInfluential
CRISPR screens in plants: approaches, guidelines, and future prospects
Gaillochet, C., Develtere, W., & Jacobs, T. B. (2021) · The Plant Cell · doi:10.1093/plcell/koab099 · Cited by 102 · see citing works
The application of a heat‐inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G. hirsutum) plants
Wang, Q., Alariqi, M., Wang, F., Li, B., Ding, X. L., Rui, H., Li, Y., Xu, Z., Qin, L., Sun, L., et al. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13417 · Cited by 101 · see citing works
“Therefore, there is interest in modifications to the CRISPR/Cas9 system to reduce off-target effects, improve the accuracy of gene editing, and expand the scope of targeting and in finding new gene editing systems (Haeussler and Concordet, 2016; Manghwar et al., 2020; Manghwar et al., 2019).”
Background
Production of aromatic three‐line hybrid rice using novel alleles of BADH2
Hui, S., Li, H., Mawia, A. M., Zhou, L., Cai, J., Ahmad, S., Lai, C., Wang, J., Jiao, G., Xie, L., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13695 · Cited by 101 · see citing works
“The application of CRISPR/ Cas9-based gene editing technology in plant breeding is an auspicious alternative to the conventional and marker-based breeding due to the ability to precisely and efﬁciently edit genomes of diverse living organism (Fiaz et al ., 2019a; Manghwar et al ., 2019).”
CRISPR-based gene editing technology and its application in microbial engineering
Wei, J., & Li, Y. (2023) · Engineering Microbiology · doi:10.1016/j.engmic.2023.100101 · Cited by 104 · see citing works
Improving Nutritional and Functional Quality by Genome Editing of Crops: Status and Perspectives
Ku, H.-K., & Ha, S. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.577313 · Cited by 102 · see citing works
CRISPR-Based Genome Editing Tools: Insights into Technological Breakthroughs and Future Challenges
Mushtaq, M., Dar, A. A., Skalický, M., Tyagi, A., Bhagat, N., Basu, U., Bhat, B. A., Zaid, A., Ali, S., Dar, T. U. H., et al. (2021) · Genes · doi:10.3390/genes12060797 · Cited by 100 · see citing works
High‐temperature stress in crops: male sterility, yield loss and potential remedy approaches
Khan, A. H., Min, L., Ma, Y., Zeeshan, M., Jin, S., & Zhang, X. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13946 · Cited by 97 · see citing works
“…and transcription activator like effector nucleases (TALENs) have been used for genome editing, but in recent years many new and valuable techniques have emerged to edit simple and complex genomes (Chen et al., 2021b; Li et al., 2019b; Manghwar et al., 2019; Qin et al., 2020; Zhang et al., 2020b).”
Background
CRISPR-Cas9 mediated mutation in GRAIN WIDTH and WEIGHT2 (GW2) locus improves aleurone layer and grain nutritional quality in rice
Achary, V. M. M., & Reddy, M. K. (2021) · Scientific Reports · doi:10.1038/s41598-021-00828-z · Cited by 95 · see citing works
How Could the Use of Crop Wild Relatives in Breeding Increase the Adaptation of Crops to Marginal Environments?
Renzi, J. P., Coyne, C. J., Berger, J. D., von Wettberg, E., Nelson, M. N., Ureta, S., Hernández, F., Smýkal, P., & Brus, J. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.886162 · Cited by 92 · see citing works
Artificial Intelligence, Healthcare, Clinical Genomics, and Pharmacogenomics Approaches in Precision Medicine
Abdelhalim, H., Berber, A., Lodi, M. K., Jain, R., Nair, A. S., Pappu, A., Patel, K., Venkat, V., Venkatesan, C., Wable, R., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.929736 · Cited by 98 · see citing works
“CRISPR-Cas9 has aided in understanding of the disease process through establishing genetically variable disease models (Ma et al., 2014; Cho et al., 2018; Manghwar et al., 2019).”
Base editing with high efficiency in allotetraploid oilseed rape by A3A‐PBE system
Cheng, H., Hao, M., Ding, B., Mei, D., Wang, W., Wang, H., Zhou, R., Liu, J., Li, C., & Hu, Q. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13444 · Cited by 83 · see citing works
“Base-editing method has become a promising tool for precise genetic modification of important agronomic traits (Chen et al., 2019; Li et al., 2020; Manghwar et al., 2019).”
Method
Exploring the gene pool of Brassica napus by genomics‐based approaches
Hu, D., Jing, J., Snowdon, R. J., Mason, A. S., Shen, J., Meng, J., & Zou, J. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13636 · Cited by 79 · see citing works
“…used in dozens of species to create desirable traits in major crops (Ezure and Miura, 2018; Malzahn et al., 2017; Manghwar et al., 2020; Manghwar et al., 2019; Metje-Sprink et al., 2020; Zaman et al., 2019b), and dozens of gene-edited crops have been approved by a few countries…”
BackgroundMethod
CRISPR/Cas9: A Powerful Strategy to Improve CAR-T Cell Persistence
Wei, W., Chen, Z., & Wang, X. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241512317 · Cited by 83 · see citing works
Design and synthesis of DNA hydrogel based on EXPAR and CRISPR/Cas14a for ultrasensitive detection of creatine kinase MB
Chen, M., Zhang, J., Peng, Y. Y., Bai, J., Li, S., Han, D., Ren, S., Qin, K., Zhou, H., Han, T., et al. (2022) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2022.114792 · Cited by 74 · see citing works
Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
Tavakoli, K., Pour‐Aboughadareh, A., Kianersi, F., Poczai, P., Etminan, A., & Shooshtari, L. (2021) · BioTech · doi:10.3390/biotech10030014 · Cited by 73 · see citing works
Molecular evolution and functional modification of plant miRNAs with CRISPR
Deng, F., Zeng, F., Shen, Q., Abbas, A., Cheng, J., Jiang, W. J., Chen, G. J., Shah, A. N., Holford, P., Tanveer, M., et al. (2022) · Trends in Plant Science · doi:10.1016/j.tplants.2022.01.009 · Cited by 74 · see citing works
Advancing organelle genome transformation and editing for crop improvement
Li, S., Chang, L., & Zhang, J. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100141 · Cited by 52 · see citing works
“2 Plant Communications 2, 100141, March 8 2021 a 2021 The Auth regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein (Cas) system, have been used to edit nuclear genomes for crop improvement (Chen et al., 2019; Manghwar et al., 2019; Veillet et al., 2020).”
Bacteroides fragilis Toxin Induces Intestinal Epithelial Cell Secretion of Interleukin-8 by the E-Cadherin/β-Catenin/NF-κB Dependent Pathway
Lee, C.-G., Hwang, S., Gwon, S.-Y., Park, C., Jo, M., Hong, J.-E., & Rhee, K. (2022) · Biomedicines · doi:10.3390/biomedicines10040827 · Cited by 72 · see citing works
“To elucidate the associations within BFT induced cellular signaling, the CRISPR-Cas9 gene editing system has the advantage of generating permanent, inherited knockout cell lines [48].”
Background
Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)
Wang, G., Xu, Z., Wang, F., Huang, Y., Xin, Y., Liang, S., Li, B., Si, H., Sun, L., Wang, Q., et al. (2022) · BMC Biology · doi:10.1186/s12915-022-01232-3 · Cited by 71 · see citing works
Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.)
Guan, L., Liu, R., Xu, R., Varshney, R. K., Ding, H., Li, M., Yan, X., Huang, S., Li, J., Wang, D., et al. (2022) · The Crop Journal · doi:10.1016/j.cj.2022.04.011 · Cited by 70 · see citing works
Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species
Khwatenge, C. N., & Nahashon, S. N. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.627714 · Cited by 72 · see citing works
An Insight into Modern Targeted Genome-Editing Technologies with a Special Focus on CRISPR/Cas9 and its Applications
Akram, F., Sahreen, S., Aamir, F., Haq, I. U., Malik, K. A., Imtiaz, M., Naseem, W., Nasir, N., & Waheed, H. M. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00501-4 · Cited by 70 · see citing works
Genome-editing in millets: current knowledge and future perspectives
Ceasar, S. A. (2021) · Molecular Biology Reports · doi:10.1007/s11033-021-06975-w · Cited by 67 · see citing works
The Art of Finding the Right Drug Target: Emerging Methods and Strategies
Jia, Z.-C., Yang, X., Wu, Y., Li, M., Das, D., Chen, M., & Wu, J. (2024) · Pharmacological Reviews · doi:10.1124/pharmrev.123.001028 · Cited by 67 · see citing works
Development of Atropa belladonna L. Plants with High-Yield Hyoscyamine and without Its Derivatives Using the CRISPR/Cas9 System
Zeng, L., Zhang, Q., Jiang, C., Zheng, Y., Zuo, Y., Qin, J., Liao, Z., & Deng, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22041731 · Cited by 66 · see citing works
Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing
Hussain, A., Ding, X. L., Alariqi, M., Manghwar, H., Hui, F., Li, Y., Cheng, J., Wu, C., Cao, J., & Jin, S. (2021) · Plants · doi:10.3390/plants10040621 · Cited by 66 · see citing works
Biosynthesis of 2-Acetyl-1-pyrroline in Fragrant Rice: Recent Insights into Agro-management, Environmental Factors, and Functional Genomics
Imran, M., Shafiq, S., Ashraf, U., Qi, J., Mo, Z., & Tang, X. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c07934 · Cited by 64 · see citing works
Systems-based rice improvement approaches for sustainable food and nutritional security
Verma, V. C., Vishal, B., Kohli, A., & Kumar, P. P. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02790-6 · Cited by 69 · see citing works
Novel Strategies to Combat the Emerging Drug Resistance in Human Pathogenic Microbes
Qadri, H., Shah, A. H., & Mir, M. A. (2020) · Current Drug Targets · doi:10.2174/1389450121666201228123212 · Cited by 62 · see citing works
Novel and emerging biotechnological crop protection approaches
Giudice, G., Moffa, L., Varotto, S., Cardone, M. F., Bergamini, C., Lorenzis, G. D., Velasco, R., Nerva, L., & Chitarra, W. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13605 · Cited by 63 · see citing works
“Once the target sequence is recognized, the endonuclease introduces a double-strand DNA (dsDNA) break (DSB) and induces subsequent activation of the DNA repair pathway (Manghwar et al., 2019).”
Background
Engineering Properties of Sweet Potato Starch for Industrial Applications by Biotechnological Techniques including Genome Editing
Lyu, R., Ahmed, S., Fan, W., Yang, J. M., Wu, X., Zhou, W., Zhang, P., Yuan, L., & Wang, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22179533 · Cited by 67 · see citing works
A review on emerging technologies and machine learning approaches for sustainable production of biofuel from biomass waste
Sharmila, V. G., Shanmugavel, S. P., & Banu, J. R. (2023) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2023.106997 · Cited by 61 · see citing works
Futuristic avenues of metabolic engineering techniques in bioremediation
Sharma, B., & Shukla, P. (2020) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2080 · Cited by 60 · see citing works
Bacteriophage strategies for overcoming host antiviral immunity
Gao, Z., & Feng, Y. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1211793 · Cited by 62 · see citing works
“First, fundamental research into interactions between phages and bacterial hosts expanded biotechnology tools and helped evolve and screen phages or strains with designated resistance, which have underpinned the development of many fields, including but not limited to gene editing (Adli, 2018; Manghwar et al., 2019),…”
Background
State-of-the-Art in CRISPR Technology and Engineering Drought, Salinity, and Thermo-tolerant crop plants
Chennakesavulu, K., Singh, H., Trivedi, P. K., Jain, M., & Yadav, S. R. (2022) · Plant Cell Reports · doi:10.1007/s00299-021-02681-w · Cited by 58 · see citing works
Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Ursache, R., Fujita, S., Tendon, V. D., & Geldner, N. (2021) · Plant Methods · doi:10.1186/s13007-021-00811-9 · Cited by 58 · see citing works
“It is a very valuable tool in studying gene function, breaking the redundancy in multigene families and developing new plant traits (Liu and Fan, 2014; Ma et al., 2016; Manghwar et al., 2019; Atkins and Voytas, 2020; Gao, 2021; Sukegawa et al., 2021).”
Method
Functional nucleic acid-based fluorescent probes for metal ion detection
Xu, J., Jiang, R., Feng, Y., Liu, Z., Huang, J. L., Ma, C., & Wang, K. (2022) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2022.214453 · Cited by 56 · see citing works
Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants
Ding, X. L., Yu, L., Chen, L., Li, Y., Zhang, J., Sheng, H., Ren, Z., Li, Y., Yu, X., Jin, S., et al. (2022) · Cells · doi:10.3390/cells11193045 · Cited by 57 · see citing works
Detection of antimicrobial resistance via state-of-the-art technologies versus conventional methods
Elbehiry, A., Marzouk, E., Abalkhail, A., Abdelsalam, M. H., Mostafa, M. E., Alasiri, M., Ibrahem, M. D., Ellethy, A. T., Almuzaini, A. M., Aljarallah, S. N., et al. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1549044 · Cited by 58 · see citing works
Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field
Balestrini, R., Brunetti, C., Cammareri, M., Caretto, S., Cavallaro, V., Cominelli, E., Palma, M. D., Docimo, T., Giovinazzo, G., Grandillo, S., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22062887 · Cited by 59 · see citing works
CRISPR/Cas9-Mediated Targeted Mutagenesis of CYP93E2 Modulates the Triterpene Saponin Biosynthesis in Medicago truncatula
Confalonieri, M., Carelli, M., Gianoglio, S., Moglia, A., Biazzi, E., & Tava, A. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.690231 · Cited by 55 · see citing works
“Of these, the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system due to its high efficiency, simplicity and specificity has become a powerful and effective tool for targeted genome editing and research into gene function in plants (Doudna and Charpentier, 2014; Manghwar et al., 2019).”
Background
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated genome‐editing toolkit to enhance salt stress tolerance in rice and wheat
Nazir, R., Mandal, S., Mitra, S., Ghorai, M., Das, N., Jha, N. K., Majumder, M., Pandey, D. K., & Dey, A. (2022) · Physiologia Plantarum · doi:10.1111/ppl.13642 · Cited by 55 · see citing works
CRISPR/Cas9-Mediated genetically edited ornamental and aromatic plants: A promising technology in phytoremediation of heavy metals
Nayeri, S., Dehghanian, Z., Lajayer, B. A., Thomson, A., Astatkie, T., & Price, G. W. (2023) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2023.139512 · Cited by 56 · see citing works
A FLASH pipeline for arrayed CRISPR library construction and the gene function discovery of rice receptor-like kinases
Chen, K., Ke, R., Du, M., Yi, Y., Chen, Y., Wang, X., Lu, Y., Liu, H., Hou, X., Xiong, L., et al. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.09.015 · Cited by 53 · see citing works
Intelligent nanotherapeutic strategies for the delivery of CRISPR system
Chen, C., Zhong, W., Du, S., Li, Y., Zeng, Y., Liu, K., Yang, J., Guan, X., & Han, X. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.12.013 · Cited by 54 · see citing works
Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
Veillet, F., Durand, M., Kroj, T., Césari, S., & Gallois, J. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100102 · Cited by 58 · see citing works
“So far, most genome-editing applications in plants have focused on the production of knockout mutants for single or multiple genes (Manghwar et al., 2019).”
Background
Toward a smart skin: Harnessing cuticle biosynthesis for crop adaptation to drought, salinity, temperature, and ultraviolet stress
Liu, L., Wang, X., & Chang, C. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.961829 · Cited by 52 · see citing works
A CRISPR way for accelerating cereal crop improvement: Progress and challenges
Basu, U., Riaz Ahmed, S., Bhat, B. A., Anwar, Z., Ali, A., Ijaz, A., Gulzar, A., Bibi, A., Tyagi, A., Nebapure, S. M., et al. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2022.866976 · Cited by 51 · see citing works
“The evolution of various GE technologies such as transcription activator-like effector nucleases (TALEN) and zinc finger nuclease (ZFN) have been previously used in the intended modification of human, animal, and plant cell genomes (Shukla et al., 2009; Zhang et al., 2010; Davies et al., 2017; Adli, 2018; Kannan et al…”
Method
Recent advances in the development of Aspergillus for protein production
Li, Q., Lu, J., Zhang, G., Liu, S., Zhou, J., Du, G., & Chen, J. (2022) · Bioresource Technology · doi:10.1016/j.biortech.2022.126768 · Cited by 51 · see citing works
A New Method Based on LAMP-CRISPR–Cas12a-Lateral Flow Immunochromatographic Strip for Detection
Xu, H., Tang, H., Li, R., Xia, Z., Yang, W., Zhu, Y., Liu, Z., Lu, G., Ni, S., & Shen, J. (2022) · Infection and Drug Resistance · doi:10.2147/idr.s348456 · Cited by 51 · see citing works
“The amplified products were trans-cleaved by Cas12a to observe the results.(15)”
Method
CRISPR/Cas9 Based Site-Specific Modification of FAD2 cis-Regulatory Motifs in Peanut (Arachis hypogaea L)
Neelakandan, A. K., Wright, D. A., Traore, S. M., Chen, X., Spalding, M. H., & He, G. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.849961 · Cited by 51 · see citing works
“This technology is used to generate gene knockouts when coding regions are targeted, therefore, this approach has been widely used for plant genome editing applications (Manghwar et al., 2019).”
Method
An overview of genome engineering in plants, including its scope, technologies, progress and grand challenges
Sufyan, M., Daraz, U., Hyder, S., Zulfiqar, U., Iqbal, R., Eldin, S. M., Rafiq, F., Mahmood, N., Shahzad, K., Uzair, M., et al. (2023) · Functional & Integrative Genomics · doi:10.1007/s10142-023-01036-w · Cited by 51 · see citing works
CRISPR/Cas9 assisted stem cell therapy in Parkinson's disease
Pinjala, P., Tryphena, K. P., Prasad, R., Khatri, D. K., Sun, W., Singh, S. B., Gugulothu, D. B., Srivastava, S., & Vora, L. K. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00381-y · Cited by 50 · see citing works
“Finally, Cas9 will cleave near the PAM site proximate to the 3′ end of the target region [53].”
Background
CRISPR: History and perspectives to the future
Kozovská, Z., Rajcaniova, S., Munteanu, P., Dzacovska, S., & Demková, L. (2021) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2021.111917 · Cited by 54 · see citing works
CRISPR/Cas-Mediated Resistance against Viruses in Plants
Khan, Z. A., Kumar, R., & Dasgupta, I. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23042303 · Cited by 51 · see citing works
Construction of Host Plant Insect‐Resistance Mutant Library by High‐Throughput CRISPR/Cas9 System and Identification of A Broad‐Spectrum Insect Resistance Gene
Sun, L., Alariqi, M., Wang, Y., Wang, Q., Xu, Z., Zafar, M. N., Yang, G., Jia, R., Hussain, A., Chen, Y., et al. (2023) · Advanced Science · doi:10.1002/advs.202306157 · Cited by 50 · see citing works
Delivery of RNAs to Specific Organs by Lipid Nanoparticles for Gene Therapy
Godbout, K., & Tremblay, J. P. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14102129 · Cited by 50 · see citing works
Detection of Infectious Viruses Using CRISPR-Cas12-Based Assay
Talwar, C. S., Park, K., Ahn, W., Kim, Y.-S., Kwon, O. S., Yong, D., Kang, T., & Woo, E. (2021) · Biosensors · doi:10.3390/bios11090301 · Cited by 47 · see citing works
Introduction to Artificial Intelligence
Ghosh, M., & Thirugnanam, A. (2021) · Studies in big data · doi:10.1007/978-981-16-0415-7_2 · Cited by 45 · see citing works
Advances in genomics and genome editing for breeding next generation of fruit and nut crops
Savadi, S., Mangalassery, S., & Sandesh, M. S. (2021) · Genomics · doi:10.1016/j.ygeno.2021.09.001 · Cited by 46 · see citing works
Engineering drought-tolerant apple by knocking down six GH3 genes and potential application of transgenic apple as a rootstock
Jiang, L., Shen, W., Liu, C., Tahir, M. M., Li, X., Zhou, S., Ma, F., & Guan, Q. (2022) · Horticulture Research · doi:10.1093/hr/uhac122 · Cited by 45 · see citing works
Large-scale genome editing in plants: approaches, applications, and future perspectives
Liu, T., Zhang, X., Li, K., Yao, Q., Zhong, D., Deng, Q., & Lu, Y. (2023) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2022.102875 · Cited by 46 · see citing works
Next-generation CRISPR-based diagnostic tools for human diseases
Wang, T., Wang, Z., Bai, L., Zhang, X., Feng, J., Qian, C., Wang, Y., & Wang, R. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117328 · Cited by 45 · see citing works
Mitochondria: emerging therapeutic strategies for oocyte rescue
Jiang, Z., & Shen, H. (2021) · Reproductive Sciences · doi:10.1007/s43032-021-00523-4 · Cited by 44 · see citing works
CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement
Naik, B. J., Shimoga, G. D., Kim, S.-C., Manjulatha, M., Palem, R. R., Palem, R. R., Kumar, M., Kim, S., & Lee, S. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.843575 · Cited by 44 · see citing works
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
The Critical Role of Small RNAs in Regulating Plant Innate Immunity
Waheed, S., Anwar, M., Saleem, M. A., Wu, J., Tayyab, M., & Hu, Z. (2021) · Biomolecules · doi:10.3390/biom11020184 · Cited by 44 · see citing works
CRISPR-Based Genome Editing and Its Applications in Woody Plants
Min, T., Hwarari, D., Li, D., Movahedi, A., & Yang, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231710175 · Cited by 43 · see citing works
Identification and Functional Analysis of lncRNA by CRISPR/Cas9 During the Cotton Response to Sap-Sucking Insect Infestation
Zhang, J., Li, J., Saeed, S., Batchelor, W. D., Alariqi, M., Meng, Q., Zhu, F., Zou, J., Xu, Z., Si, H., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.784511 · Cited by 42 · see citing works
Opportunities and challenges with CRISPR-Cas mediated homologous recombination based precise editing in plants and animals
Singh, S., Chaudhary, R., Deshmukh, R. K., & Tiwari, S. (2022) · Plant Molecular Biology · doi:10.1007/s11103-022-01321-5 · Cited by 42 · see citing works
“The enAsCas12a can recognize “TTTV” PAM sequence and showed two-fold higher genome editing activity compared with wild-type AsCas12a with reduced offtarget effects (Kleinstiver et al. 2019; Manghwar et al. 2019).”
Background
Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9
Assou, J., Zhang, D., Roth, K. D. R., Steinke, S., Hust, M., Reinard, T., Winkelmann, T., & Boch, J. (2021) · The Plant Journal · doi:10.1111/tpj.15584 · Cited by 42 · see citing works
“Using programmable nucleases, like CRISPR/Cas9 or TALEN, targeted random mutations and deletions can be induced (Manghwar et al., 2019).”
Method
Advancing cancer research through organoid technology
Zeng, G., Yu, Y., Wang, M., Liu, J., He, G., Yu, S., Yan, H., Yang, L., Li, H., & Peng, X. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05824-1 · Cited by 43 · see citing works
“This mechanism can lead to the mispairing of the designed single-stranded guide RNA (sgRNA) with non-target DNA sequences, resulting in off-target effects and the introduction of unexpected gene mutations [121, 122].”
Background

CRISPR/Cas9-Based Genome Editing in Plants
Zhang, Y., Ma, X., Xie, X., & Liu, Y. (2017) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2017.03.008 · Cited by 53 · see citing works
Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes
Soyars, C. L., Peterson, B. A., Burr, C. A., & Nimchuk, Z. L. (2018) · Plant and Cell Physiology · doi:10.1093/pcp/pcy079 · Cited by 50 · see citing works
[Advances in CRISPR-Cas-mediated genome editing system in plants].
Wang, C., & Wang, K. (2017) · PubMed · doi:10.13345/j.cjb.170170 · Cited by 1 · see citing works
Breakthrough in CRISPR/Cas system: Current and future directions and challenges
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The CRISPR/Cas9 system and its applications in crop genome editing
Bao, A., Burritt, D. J., Chen, H., Zhou, X., Cao, D., & Tran, L.-S. P. (2019) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2018.1554621 · Cited by 185 · see citing works

Authors: Manghwar, H., Lindsey, K., Zhang, X., & Jin, S.
Affiliations: Huazhong Agricultural University (CN)
Durham University (GB)
Corresponding author: Zhang, Xianlong
Jin, Shuangxia
ORCID: Manghwar, Hakim
Lindsey, Keith
Zhang, Xianlong
Jin, Shuangxia
Venue: Trends in Plant Science
Publisher: Elsevier BV
Published: 2019-12
Volume: 24
Issue: 12
Pages: 1102-1125
ISSN: 1360-1385 (ISSN-L)
DOI: 10.1016/j.tplants.2019.09.006
OpenAlex: W2984281074
Cited by: 529
References: 140
Citations by year: 2026: 49 · 2025: 84 · 2024: 94 · 2023: 91 · 2022: 76 · 2021: 91 · 2020: 43 · 2014: 1
FWCI: 4.65
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-nd
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by-nc-nd/4.0/ (vor)
Locations: Trends in Plant Science (publishedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
Durham Research Online (Durham University) (publishedVersion, other-oa)
Durham Research Online (Durham University) (acceptedVersion, other-oa)
Durham Research Online (Durham University) (submittedVersion, cc-by-nc-nd)
Funders: Ministry of Science and Technology of the People's Republic of China
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords: CRISPR/Cas9, genome editing, base editing, genome editing tools
MeSH terms: Gene Editing, Crops, Agricultural, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems

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CRISPR/Cas system: recent advances and future prospects for genome editing

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