The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Engineering herbicide resistance via prime editing in rice
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Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013).In plants homology-directed repair is inefficient, limiting our ability to make precise edits of the
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“For instance, the rice gene Oryza sativa acetolactate synthase (OsALS) was targeted to develop resistance to the herbicide bispyribac sodium (Butt et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Butt et al., 2020
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Search-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767 · cited 5×
“Researchers have developed several prime editing strategies: in PE1, wild type M-MLV RT fused to the C terminus of Cas9 (H840A) nickase; in PE2, Cas9 (H840A) with pentamutant M-MLV RT (D200N/ L603W/ T330P/ T306K/ W313F); in PE3, a PE2 prime editor with additional simple gRNA to simultaneously nick the non-edited stran…”
BackgroundMethodInfluentialTargeted genome modification of crop plants using a CRISPR-Cas system
Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J. J., Qiu, J.-L., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2650 · Cited by 1,810 · cited 1×
“Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013).”
BackgroundBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675 · cited 1×
“Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence and the possibility of off-target mutations at other loci (Rees and Liu, 2018).”
BackgroundBoosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
Xie, K., Minkenberg, B., & Yang, Y. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420294112 · Cited by 1,557 · cited 1×
“We designed the sgRNA to target OsALS at a distance of +55 from the pegRNA and expressed this sgRNA from the OsU3 promoter using the polycistronic tRNA-gRNA system (Butt et al., 2017; Xie et al., 2015) (Figure 1a).”
MethodRegulation of DNA double-strand break repair pathway choice
Shrivastav, M., De Haro, L. P., & Nickoloff, J. A. (2008) · Cell Research · doi:10.1038/cr.2007.111 · Cited by 1,329
Prime genome editing in rice and wheat
Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · cited 1×
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultPrecise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing
Li, H., Li, J., Chen, J., Yan, L., & Xia, L. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.03.011 · Cited by 202 · cited 1×
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultPlant Prime Editors Enable Precise Gene Editing in Rice Cells
Tang, X. D., Sretenovic, S., Ren, Q., Jia, X., Li, M., Fan, T., Yin, D., Xiang, S., Guo, Y., Liu, L., et al. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.03.010 · Cited by 194 · cited 1×
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultAli, Z., Shami, A., Sedeek, K., Kamel, R., Alhabsi, A., Tehseen, M., Hassan, N., Butt, H., Kababji, A. M., Hamdan, S. M., et al. (2020) · Communications Biology · doi:10.1038/s42003-020-0768-9 · Cited by 142 · cited 1×
“In plants, homology-directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017).”
BackgroundSelf-citationEfficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
Butt, H., Eid, A., Ali, Z., Atia, M. A. M., Mokhtar, M. M., Hassan, N., Lee, C. M., Bao, G., & Mahfouz, M. M. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01441 · Cited by 137 · cited 4×
“A single amino acid change (W548L) in ALS results in a BS-resistant phenotype (Butt et al., 2017).”
BackgroundMethodInfluentialSelf-citation
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Abdallah, N. A. (2025) · Earth and environmental sciences library · doi:10.1007/978-3-031-74897-4_8
Genome Editing to Accelerate Biotic Stress Resistance in Crop Plants
Gul, A., Aaliya, K., & Iftikhar, S. (2025) · CABI eBooks · doi:10.1079/9781800622517.0013
Genome Editing for Herbicide Tolerance in Crop Plants
Ullah, A., & Shah, S. W. O. (2025) · CABI eBooks · doi:10.1079/9781800622517.0015
Ansarì, M. S., Khan, A. A., & Ulhassan, Z. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-14139-3.00007-5
CRISPR-edited plants for herbicide resistance: Methods and applications
Hu, H. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-43920-9.00008-7
Patel, E., Das, P., Hazra, S., Malik, P., Arora, S., Kaur, A., & Sandhu, J. S. (2025) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-025-10603-8
Genome Editing: CRISPR-CAS System: A Promising Diagnostic Tool for Plant Pathogens
Abd-Elsalam, K. A., Ahmad, A., AboDalam, H., et al. (2026) · Sustainability Sciences in Asia and Africa · doi:10.1007/978-981-95-6722-5_3
Enzyme engineering in medicinal plants: Enhancements in the yield of secondary metabolites
Singh, M., Soni, R., Singh, A., Kumar, R., Rana, S., Bano, F., & Gupta, V. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-34129-8.00023-7
Prime editing: evolution of CRISPR–Cas system for a robust next-generation genome editing in plants
Bargoti, T., Nain, V., & Singh, D. (2026) · Planta · doi:10.1007/s00425-026-05040-9 · Cited by 1
Dutta, S., Sadhukhan, R., Das, K., Maji, A., & Kundagrami, S. (2026) · Discover Plants. · doi:10.1007/s44372-026-00780-0
“On a medium containing 0.75 mM bispyribac-sodium, HR shoots were produced [150].”
InfluentialAI-guided CRISPR in crop yield enhancement
Musazade, E., Mrisho, I. I., Zhang, D., Gao, J., Cheng, Z., & Feng, X. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-44079-3.00005-x
Zhang, L., & Li, M. (2026) · Journal of Integrative Plant Biology · doi:10.1111/jipb.70386
Next-generation biotechnology for improving abiotic stress tolerance in rice
Kayess, M. O., Shefa, M. A., & Islam, T. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-33465-8.00033-5
Climate-resilient rice crop improvement using CRISPR–Cas technology
Kafi, M. A. A., Gupta, D. R., & Islam, T. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-33465-8.00032-3
Developing rice tolerance to abiotic stress through gene editing technologies
Islam, S., Gupta, D. R., & Islam, T. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-33465-8.00003-7
From feasibility to predictability: prime editing redefines precision breeding in plants
Awan, M. J. A., Qari, S. H., Yin, Z., Ahmad, N., & Mansoor, S. (2026) · Trends in Plant Science · doi:10.1016/j.tplants.2026.08.008
Junejo, Y., Ali, D., Kolhi, M. L., Memon, F. U., Yu, H., Tariq, H., Ashraf, Z. U., Liu, X., Wang, Y., & Xiao, H. (2026) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2026.107357
Metadata
- Authors
- Butt, H., Rao, G. S., Sedeek, K., Aman, R., Kamel, R., & Mahfouz, M.
- Affiliations
- King Abdullah University of Science and Technology (SA)
- Corresponding author
- Mahfouz, Magdy
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2020-06-11
- Volume
- 18
- Issue
- 12
- Pages
- 2370-2372
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- PMC
- PMC7680537
- OpenAlex
- W3025159539
- Semantic Scholar
- 707270480172698cd9a447b013ee5db6c1cc92fa
- Cited by
- 220
- References
- 10
- Citations by year
- 2026: 26 · 2025: 33 · 2024: 32 · 2023: 28 · 2022: 51 · 2021: 38 · 2020: 12
- FWCI
- 10.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Biotechnology Journal (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) (submittedVersion, cc-by)
- Funders
- King Abdullah University of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Weed Control and Herbicide Applications
- Keywords
- prime editing, herbicide resistance, rice, CRISPR/Cas9, genome editing, homology-directed repair, plant genome editing
- MeSH terms
- Gene Editing, Oryza, Herbicide Resistance, CRISPR-Cas Systems
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