Advanced domestication: harnessing the precision of gene editing in crop breeding
Open accessAbstract
Human population growth has increased the demand for food crops, animal feed, biofuel and biomaterials, all the while climate change is impacting environmental growth conditions. There is an urgent need to develop crop varieties which tolerate adverse growth conditions while requiring fewer inputs. Plant breeding is critical to global food security and, while it has benefited from modern technologies, it remains constrained by a lack of valuable genetic diversity, linkage drag, and an effective way to combine multiple favourable alleles for complex traits. CRISPR/Cas technology has transformed genome editing across biological systems and promises to transform agriculture with its high precision, ease of design, multiplexing ability and low cost. We discuss the integration of CRISPR/Cas-based gene editing into crop breeding to advance domestication and refine inbred crop varieties for various applications and growth environments. We highlight the use of CRISPR/Cas-based gene editing to fix desirable allelic variants, generate novel alleles, break deleterious genetic linkages, support pre-breeding and for introgression of favourable loci into elite lines.
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Base editing technologies have demonstrated high efficiency and specificity in various crops, including rice and Arabidopsis, allowing for the precise development of desirable traits compared to conventional breeding techniques.
“Moreover, base editing technologies have demonstrated high efficiency and specificity in various crops, including rice and Arabidopsis, allowing for the precise development of desirable traits compared to conventional breeding techniques (Ren et al., 2021; Lyzenga et al., 2021).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Lyzenga et al., 2021
The ‘Advances in CRISPR technology’ section (page unavailable) describes base editing as targeted single-nucleotide conversion and says it has been deployed in rice and other crops. It does not establish high efficiency and specificity for base editing in those crops, or report base editing in Arabidopsis. The crop-improvement discussion supports its potential to produce desirable alleles, but not the full claim as stated. (LLM, conf=0.86)
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Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
“Moreover, base editing technologies have demonstrated high ef fi ciency and speci fi city in various crops, including rice and Arabidopsis, allowing for the precise development of desirable traits compared to conventional breeding techniques (Ren et al., 2021; Lyzenga et al., 2021).”
- Authors
- Lyzenga, W. J., Pozniak, C. J., & Kagale, S.
- Affiliations
- University of Saskatchewan (CA)
- National Research Council Canada (CA)
- Corresponding author
- Kagale, Sateesh
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2021-03-25
- Volume
- 19
- Issue
- 4
- Pages
- 660-670
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- PMC
- PMC8051614
- OpenAlex
- W3133490491
- Cited by
- 85
- References
- 126
- Citations by year
- 2026: 13 · 2025: 18 · 2024: 22 · 2023: 10 · 2022: 12 · 2021: 10
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Biotechnology Journal (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- NPARC (submittedVersion, cc-by)
- Funders
- Agriculture and Agri-Food Canada
- Western Grains Research Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- CRISPR gene editing, crop breeding, plant domestication, linkage drag, introgression, pre-breeding, novel alleles, genetic diversity, food security, climate-resilient crops
- MeSH terms
- Plant Breeding, Domestication, Gene Editing, Genome, Plant, CRISPR-Cas Systems
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