CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae
Open accessAbstract
Genome editing tools are important for functional genomics research and biotechnology applications. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) system for gene knockout has emerged as the most effective genome-editing tool. It has previously been reported that, in rice plants, knockdown of the Os8N3 gene resulted in enhanced resistance to Xanthomonas oryzae pv. oryzae ( Xoo ), while displaying abnormal pollen development. The CRISPR/Cas9 system was employed to knockout rice Os8N3 , in order to confer enhanced resistance to Xoo . Analysis of the genotypes and edited Os8N3 in T 0 , T 1 , T 2 , and T 3 transgenic rice plants showed that the mutations were transmitted to subsequent generations, and homozygous mutants displayed significantly enhanced resistance to Xoo . Stable transmission of CRISPR/Cas9-mediated Os8N3 gene editing without the transferred DNA (T-DNA) was confirmed by segregation in the T 1 generation. With respect to many investigated agronomic traits including pollen development, there was no significant difference between homozygous mutants and non-transgenic control plants under greenhouse growth conditions. Data from this study indicate that the CRISPR/Cas9-mediated Os8N3 edition can be successfully employed for non-transgenic crop improvements.
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Single or multiple gene knockouts have been successfully observed in maize, barley, wheat, and rice.
“Gene knockout is the widely used application of CRISPR/Cas9, and single or multiple gene knockouts have been successfully observed in maize, barley, wheat, and rice (Li et al., 2013;Doll et al., 2019;Kim et al., 2019;Lawrenson and Harwood, 2019;Křenek et al., 2021;Zhang et al., 2021;Karunarathne et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Kim et al., 2019
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CRISPR/Cas: A powerful tool for gene function study and crop improvement
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“…widely used applica‐ tion of CRISPR/Cas9, and single or multiple gene knockouts have been successfully observed in maize, barley, wheat, and rice (Li et al., 2013; Doll et al., 2019; Kim et al., 2019; Lawrenson and Harwood, 2019; Křenek et al., 2021; Zhang et al., 2021; Karunarathne et al., 2022).”
- Authors
- Kim, Y.-A., Moon, H., & Park, C.-J.
- Affiliations
- Sejong University (KR)
- Corresponding author
- Park, Chang-Jin
- Venue
- Rice
- Published
- 2019-08-24
- Volume
- 12
- Issue
- 1
- Pages
- 67-67
- ISSN
- 1939-8425 (ISSN-L)
- 1939-8433
- PMC
- PMC6708514
- OpenAlex
- W2969916037
- Cited by
- 149
- References
- 57
- Citations by year
- 2026: 11 · 2025: 18 · 2024: 24 · 2023: 24 · 2022: 32 · 2021: 24 · 2020: 13 · 2019: 2
- FWCI
- 5.87
- Type
- journal article
- Language
- en
- Update notices
- correction (2019-09-13)
- Open access
- gold
- doi.org
- Locations
- Rice (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Basic Research Program of National Research Foundation of Korea (NRF-2017R1D1A1B03032215)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- Xanthomonas oryzae pv. oryzae, bacterial blight resistance, CRISPR/Cas9 genome editing, gene knockout, pollen development
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