Impact of food restriction on the expression of the adiponectin system and genes in the hypothalamic-pituitary-ovarian axis of pre-pubertal ewes
Abstract
Adiponectin, a cytokine secreted typically by adipocytes, has been implicated as a molecular switch between female reproduction and energy balance. The present study was undertaken to investigate the expression of adiponectin system and patterns of genes in the hypothalamic-pituitary-ovary (HPO) axis of food-restricted pre-pubertal ewes. Eighteen 2-month-old female ewes were assigned to 3 groups after a pre-feeding ad libitum for 10 days (six in each group): the control group (C), the low-food-restricted group (LR) and the high-food-restricted group (HR), which were fed with 100%, 70% and 50% of ad libitum food intake, respectively. The hypothalamus, pituitary, ovary and serum were collected after food restriction for 2 months. Results by ELISA showed that food restriction increased serum adiponectin concentrations. Quantitative real-time PCR showed that the gene transcriptions for adiponectin receptor 1 (AdipoR1) and 2 (AdipoR2) were enhanced in the hypothalamic-pituitary-ovarian (HPO) axis, while KISS-1/GPR-54 and gonadotropin-releasing hormone (GnRH) in the hypothalamus and luteinizing hormone β-subunit (LHβ) and follicle-stimulating hormone β-subunit (FSHβ) in the pituitary were reduced after food restriction. Immunohistochemistry results demonstrated that AdipoR1 localized in the oocytes of follicles in the ovary. These results suggest that the alterations in the expression of adiponectin and its receptors in response to food restriction might negatively influence the HPO axis.
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Gnrh genes are down-regulated in animals exposed to fasting or food restriction.
“Gnrh genes are down-regulated in animals exposed to fasting or food restriction (45, 46).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [46]
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- Authors
- Wang, R., Kuang, M., Nie, H., Bai, W., Sun, L., Wang, F., Mao, D., & Wang, Z.
- Affiliations
- Nanjing Agricultural University (CN)
- Corresponding author
- Mao, D
- Wang, Z
- Venue
- Reproduction in Domestic Animals
- Publisher
- Wiley
- Published
- 2016-07-13
- Volume
- 51
- Issue
- 5
- Pages
- 657-664
- ISSN
- 0044-5371 (ISSN-L)
- 0936-6768
- 1439-0531
- OpenAlex
- W2463707531
- Cited by
- 18
- References
- 49
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 2
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Reproduction in Domestic Animals (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Jiangsu Key Agricultural Technology Support Program (BE2014330)
- Agroscientific Research in the Public Interest (201303143-06)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Reproductive Physiology in Livestock
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- adiponectin, dietary restriction, hypothalamic-pituitary-ovarian axis, adiponectin receptors, AdipoR1, AdipoR2, gonadotropin-releasing hormone, luteinizing hormone beta subunit, follicle-stimulating hormone beta subunit, energy balance, female reproduction, oocytes
- MeSH terms
- Animals, Energy Intake, Female, Food Deprivation, Follicle Stimulating Hormone, Hypothalamo-Hypophyseal System, Luteinizing Hormone, Gonadotropin-Releasing Hormone, Ovary, RNA, Messenger, Sexual Maturation, Sheep, Gene Expression Regulation, Developmental, Adiponectin, Receptors, Adiponectin, Kisspeptins
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