Glutamate receptor channels in isolated patches from CA1 and CA3 pyramidal cells of rat hippocampal slices.
Open accessAbstract
1. Currents activated by glutamate receptor (GluR) agonists were recorded from outside-out patches isolated from the soma of visually identified pyramidal neurones of the CA3 and CA1 region of rat hippocampal slices. alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), L-glutamate (L-Glu), and kainate (KA) were delivered either by bath application through perfusion of the recording chamber or by rapid application via a piezo-driven two-barrelled fast application system. 2. Bath application of each of the three agonists activated inward currents in all patches (n = 134) at holding potentials of -50 or -60 mV. The current amplitude increased in size between 3 to 30 microM-AMPA and 100 microM to 1 mM-KA. With this slow mode of bath application, the responses showed no apparent desensitization even at saturating concentrations of AMPA (30 microM) and KA (1 mM). 3. The ratio of currents activated by 30 microM-AMPA and 300 microM-KA showed a characteristic difference between CA3 and CA1 neurones. The ratio was 0.242 +/- 0.028 (mean +/- S.E.M., n = 16) for CA3 cell patches and 0.097 +/- 0.012 (n = 8) for CA1 cell patches indicating that GluRs in the two cell populations are different. 4. The steady-state current-voltage relations (I-Vs) for AMPA- and KA-activated currents showed pronounced outward rectification for both cell types (when the main cations are Na+ in the bath and Cs+ in the pipette solution). The current reversed close to 0 mV and the ratio of chord conduct…
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The current amplitudes showed a supralinear dependence on radiation power, with a power exponent of 2.5 ± 0.6 (mean ± s.d., n = 7).
“The current amplitudes showed a supralinear (power exponent, 2.5 ± 0.6, mean ± s.d., n = 7) dependence on the radiation power, as predicted (Fig. 1i) both by the two-photon excitation30 of MNI-glutamate and by the cooperativity (1.2–1.7) of glutamate binding to AMPA receptors31 (2pEPSCs were CNQX (6-cyano-7-nitroquinoxaline)-sensitive).”
From Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons (2001) · cites this paper as [31]
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Two types of kainate response in cultured rat hippocampal neurons
Ozawa, S., Iino, M., & Tsuzuki, K. (1991) · Journal of Neurophysiology · doi:10.1152/jn.1991.66.1.2 · Cited by 115 · see citing works
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Control of Kinetic Properties of AMPA Receptor Channels by Nuclear RNA Editing
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Self-citationMiniature synaptic events maintain dendritic spines via AMPA receptor activation
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- Authors
- Jonas, P., & Sakmann, B.
- Affiliations
- Max Planck Institute for Medical Research (DE)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 1992-09
- Volume
- 455
- Issue
- 1
- Pages
- 143-171
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1175637
- OpenAlex
- W2026294888
- Cited by
- 271
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 7 · 2023: 1 · 2021: 3 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 3 · 2015: 5 · 2014: 4 · 2013: 3 · 2012: 2
- FWCI
- 9.82
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- glutamate receptor channels, AMPA receptors, kainate receptors, glutamate receptors, CA1 pyramidal neurons, CA3 pyramidal cells, receptor desensitization, outward rectification, ion permeability, sodium permeability, channel gating, current-voltage relation, rat hippocampus
- MeSH terms
- Action Potentials, Animals, Cell Membrane Permeability, Glutamates, Hippocampus, Ibotenic Acid, Kainic Acid, Pyramidal Tracts, Ion Transport, Receptors, Glutamate, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Glutamic Acid, Rats
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