Miniature synaptic events maintain dendritic spines via AMPA receptor activation
Abstract
We investigated the influence of synaptically released glutamate on postsynaptic structure by comparing the effects of deafferentation, receptor antagonists and blockers of glutamate release in hippocampal slice cultures. CA1 pyramidal cell spine density and length decreased after transection of Schaffer collaterals and after application of AMPA receptor antagonists or botulinum toxin to unlesioned cultures. Loss of spines induced by lesion or by botulinum toxin was prevented by simultaneous AMPA application. Tetrodotoxin did not affect spine density. Synaptically released glutamate thus exerts a trophic effect on spines by acting at AMPA receptors. We conclude that AMPA receptor activation by spontaneous vesicular glutamate release is sufficient to maintain dendritic spines.
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Blocking AMPA-R activity with NBQX significantly reduced spine density.
“Blocking AMPA-R activity with NBQX significantly reduced spine density (23)”
From AMPA receptor and GEF-H1/Lfc complex regulates dendritic spine development through RhoA signaling cascade (2009) · cites this paper as [23]
Neurotransmitters including norepinephrine, serotonin and glutamate, as well as stress-induced release of neuropeptides and steroids, can influence synapse number and function.
“Neurotransmitters, including norepinephrine,76, 77, 78 serotonin79, 80, 81 and glutamate,82,83 as well as stress-induced release of neuropeptides26,34,84,85 and steroids,86, 87, 88 can influence synapse number and function.”
From Preferential loss of dorsal-hippocampus synapses underlies memory impairments provoked by short, multimodal stress (2014) · cites this paper as [83]
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- Authors
- McKinney, R. A., Capogna, M., Dürr, R., Gähwiler, B. H., & Thompson, A. S. M.
- Affiliations
- University of Zurich (CH)
- Aarhus University (DK)
- University of Maryland, Baltimore (US)
- Corresponding author
- McKinney, R. Anne
- Venue
- Nature Neuroscience
- Published
- 1999-01
- Volume
- 2
- Issue
- 1
- Pages
- 44-49
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- DOI
- 10.1038/4548
- OpenAlex
- W1534752637
- Cited by
- 523
- References
- 37
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 8 · 2023: 12 · 2022: 3 · 2021: 10 · 2020: 10 · 2019: 7 · 2018: 10 · 2017: 22 · 2016: 10 · 2015: 20 · 2014: 19 · 2013: 24 · 2012: 28
- FWCI
- 11.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- dendritic spines, AMPA receptors, glutamate release, synaptic plasticity, CA1 pyramidal neurons, Schaffer collaterals, deafferentation, botulinum toxin, tetrodotoxin, spine density, spine length, hippocampus, vesicular glutamate release
- MeSH terms
- Afferent Pathways, Botulinum Toxins, Dendrites, Denervation, Hippocampus, Synapses, Tetrodotoxin, Receptors, AMPA, alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid, Excitatory Amino Acid Antagonists, Glutamic Acid, In Vitro Techniques
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