Human single muscle fibre function with 84 day bed‐rest and resistance exercise
Abstract
Muscle biopsies were obtained from the vastus lateralis before and after 84 days of bed-rest from six control (BR) and six resistance-exercised (BRE) men to examine slow- and fast-twitch muscle fibre contractile function. BR did not exercise during bed-rest and had a 17 and 40% decrease in whole muscle size and function, respectively. The BRE group performed four sets of seven maximal concentric and eccentric supine squats 2-3 days per week (every third day) that maintained whole muscle strength and size. Slow (MHC I) and fast (MHC IIa) muscle fibres were studied at 15 degrees C for diameter, peak force (P(o)), contractile velocity (V(o)) and force-power parameters. SDS-PAGE was performed on each single fibre after the functional experiments to determine MHC isoform composition. MHC I and IIa BR fibres were, respectively, 15 and 8% smaller, 46 and 25% weaker (P(o)), 21 and 6% slower (V(o)), and 54 and 24% less powerful after bed-rest (P MHC I/IIa) in single fibre MHC isoform composition.
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Evidence-based explanations for higher declines in muscle function are alterations in neural drive and changes in muscle fiber properties, such as thin filament density, yielding lower force per cross-sectional area.
“Evidence-based explanations for higher declines in muscle function (84) are alterations in neural drive and changes in muscle fiber properties, such as thin filament density (95) yielding lower force per cross-sectional area (84).”
From Nonuniform loss of muscle strength and atrophy during bed rest: a systematic review (2021) · cites this paper as [84]
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- Authors
- Trappe, S., Trappe, T., Gallagher, P., Harber, M., Alkner, B., & Tesch, P.
- Affiliations
- Ball State University (US)
- University of Arkansas for Medical Sciences (US)
- Karolinska Institutet (SE)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2004-05-24
- Volume
- 557
- Issue
- 2
- Pages
- 501-513
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- OpenAlex
- W1585427103
- Cited by
- 278
- References
- 1
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 11 · 2023: 14 · 2022: 17 · 2021: 15 · 2020: 7 · 2019: 20 · 2018: 11 · 2017: 10 · 2016: 11 · 2015: 13 · 2014: 11 · 2013: 14 · 2012: 12
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Physiology (publishedVersion, public-domain)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG 18409, R01AG018409, AG-00831)
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- bed rest, resistance training, vastus lateralis, type I muscle fibers, fast-twitch fibres, myosin heavy chain isoforms, muscle fiber size, muscle strength, muscle atrophy, muscle fiber type distribution, muscle fiber cross-sectional area
- MeSH terms
- Adult, Bed Rest, Humans, Isotonic Contraction, Male, Stress, Mechanical, Time Factors, Exercise, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myosin Heavy Chains, Protein Isoforms
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