Reduction in hybrid single muscle fiber proportions with resistance training in humans

Williamson, D. L.; Gallagher, P. M.; Carroll, C. C.; Raue, U.; Trappe, S. W.

doi:10.1152/jappl.2001.91.5.1955

Cited by 150Muscle Physiology and DisordersSports Performance and TrainingCardiovascular and exercise physiology

Reduction in hybrid single muscle fiber proportions with resistance training in humans

Williamson, D. L., Gallagher, P. M., Carroll, C. C., Raue, U., Trappe, S. W.·2001·Journal of Applied Physiology

DOI 10.1152/jappl.2001.91.5.1955

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Abstract

The purpose of this investigation was to examine the effects of 12 wk of progressive resistance training (PRT) on single muscle fiber myosin heavy chain (MHC; I, I/IIa, I/IIa/IIx, IIa, IIa/IIx, IIx) isoform proportions in young individuals. Young, untrained men (YM; n = 6) and women (YW; n = 6) (age = 22 +/- 1 and 25 +/- 2 yr for YW and YM, respectively) received pre- and post-PRT muscle biopsies from the right vastus lateralis for single muscle fiber MHC distribution by electrophoretic analysis (192 +/- 5 pre- and 183 +/- 6 post-fibers/subject analyzed; 4,495 fibers total). Data are presented as percentages of the total fibers analyzed per subject. The PRT protocol elicited an increase in the pure MHC IIa (Delta = + 24 and + 27; YW and YM, respectively; P < 0.05) with no change in the pure MHC I distribution. The hybrid MHC distributions decreased I/IIa/IIx (Delta = -2; YM and YW; P < 0.05), IIa/IIx (Delta = -13 and -19 for YM and YW, respectively; P < 0.05), and total hybrid fiber proportion (I/IIa + I/IIa/IIx + IIa/IIx) decreased (Delta = -19 and -30 for YM and YW, respectively; P < 0.05) with the training, as did the MHC IIx distribution (Delta = -2; YW only; P < 0.05). Alterations in the predominance of MHC isoforms within hybrid fibers (decrease in MHC I-dominant I/IIa and nondominant MHC IIa/IIx, increase in MHC IIa-dominant IIa/IIx; P < 0.05) appeared to contribute to the increase in the MHC IIa proportion. Electrophoresis of muscle cross sections revealed an approximately 7% increase (P < 0.05) in MHC IIa proportion in both groups, whereas the MHC IIx decrease by 7.5 and 11.6% post-PRT in YW and YM, respectively. MHC I proportions increase in YM by 4.8% (P < 0.05) post-PRT. These findings further support previous resistance training data in young adults with respect to the increase in the MHC IIa proportions but demonstrate that a majority of the change can be attributed to the decrease in single-fiber hybrid proportions.

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단기간의 고강도 저항성 트레이닝에 의한 근섬유의 MHC phenotype 변화
하재여, 김효정, & 김창근 (2011)

Authors: Williamson, D. L., Gallagher, P. M., Carroll, C. C., Raue, U., & Trappe, S. W.
Affiliations: Ball State University (US)
ORCID: Gallagher, P. M.
Carroll, C. C.
Trappe, S. W.
Venue: Journal of Applied Physiology
Publisher: American Physiological Society
Published: 2001-11-01
Volume: 91
Issue: 5
Pages: 1955-1961
ISSN: 0161-7567 (ISSN-L)
8750-7587
1522-1601
DOI: 10.1152/jappl.2001.91.5.1955
OpenAlex: W1846380959
Cited by: 150
References: 26
Citations by year: 2026: 4 · 2025: 6 · 2024: 1 · 2023: 8 · 2022: 3 · 2021: 6 · 2020: 15 · 2019: 9 · 2018: 5 · 2017: 5 · 2016: 5 · 2015: 8 · 2014: 5 · 2013: 4 · 2012: 6
FWCI: 1.98
Type: journal article
Language: en
Open access: closed
Locations: Journal of Applied Physiology (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute on Aging (AG154876)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords: resistance training, myosin heavy chain isoforms, hybrid muscle fibers, MHC IIa, MHC IIx, MHC class I, vastus lateralis, muscle fiber composition, sex differences, young adults, muscle hypertrophy, progressive resistance training
MeSH terms: Adult, Aging, Bicycling, Electrophoresis, Polyacrylamide Gel, Female, Humans, Male, Myosins, Physical Fitness, Muscle, Skeletal, Muscle Fibers, Skeletal, Myosin Heavy Chains

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Reduction in hybrid single muscle fiber proportions with resistance training in humans

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