Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis
Abstract
In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower‐limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN‐C, ROCK1, and FAK); and expression of PGC1a and atrophy‐related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤0.01), WDR1 (39%, P ≤0.01), sarcosin (84%, P ≤0.01), and CKM (20%, P ≤0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN‐C increased (59%, P ≤0.01); the latter also with RE (108%, P ≤0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT‐slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultra‐structural and molecular dysregulation induced by chronic disuse.—Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., Blottner, D., Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis. FASEB J. 28, 4748–4763 (2014). www.fasebj.org
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In studies on molecular mechanisms of simulated μG, the majority of regulated genes and proteins were related to insulin resistance, metabolism, and reduced protein synthesis.
“Careful studies on the molecular mechanisms revealed that the majority of regulated genes and proteins were related to insulin resistance, metabolism, and reduced protein synthesis [170,171,172].”
From Interactions between Muscle and Bone—Where Physics Meets Biology (2020) · cites this paper as [171]
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- Authors
- Salanova, M., Gelfi, C., Moriggi, M., Vasso, M., Viganò, A., Minafra, L., Bonifacio, G., Schiffl, G., Gutsmann, M., Felsenberg, D., Cerretelli, P., & Blottner, D.
- Affiliations
- Center of Space Medicine Berlin Neuromuscular Group Institute of Anatomy Charité Universitätsmedizin Berlin Berlin Germany
- University of Milan (IT)
- Institute of Molecular Bioimaging and Physiology (IT)
- University of Lausanne (CH)
- Charité – Universitätsmedizin Berlin (DE)
- National Research Council (IT)
- Corresponding author
- Gelfi, Cecilia
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2014-08-13
- Volume
- 28
- Issue
- 11
- Pages
- 4748-4763
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2158257758
- Cited by
- 68
- References
- 48
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 7 · 2019: 5 · 2018: 5 · 2017: 7 · 2016: 6 · 2015: 25 · 2014: 3
- FWCI
- 9.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Effects of Vibration on Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- disuse atrophy, superimposed vibration, resistance training, bed rest, soleus muscle, vastus lateralis, MuRF1, MuRF2, PGC-1α, MYBPC1, WDR1, troponin T, myosin light chain 2, differential proteomics
- MeSH terms
- Adult, Bed Rest, Exercise Therapy, Humans, Male, Middle Aged, Muscle Contraction, Muscular Atrophy, Vibration, Muscle, Skeletal, Proteomics, Young Adult
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