Characterization of voltage‐dependent sodium and calcium channels in mouse pancreatic A‐ and B‐cells
Open accessAbstract
Insulin and glucagon are the major hormones of the islets of Langerhans that are stored and released from the B- and A-cells, respectively. Both hormones are secreted when the intracellular cytosolic Ca2+ concentration ([Ca2+]i) increases. The [Ca2+]i is modulated by mutual inhibition and activation of different voltage-gated ion channels. The precise interplay of these ion channels in either glucagon or insulin release is unknown, owing in part to the difficulties in distinguishing A- from B-cells in electrophysiological experiments. We have established a single-cell RT-PCR method to identify A- and B-cells from the mouse. A combination of PCR, RT-PCR, electrophysiology and pharmacology enabled us to characterize the different sodium and calcium channels in mouse islet cells. In both A- and B-cells, 60% of the inward calcium current (I(Ca)) is carried by L-type calcium channels. In B-cells, the predominant calcium channel is Ca(v)1.2, whereas Ca(v)1.2 and Ca(v)1.3 were identified in A-cells. These results were confirmed by using mice carrying A- or B-cell-specific inactivation of the Ca(v)1.2 gene. In B-cells, the remaining I(Ca) flows in equal amounts through Ca(v)2.1, Ca(v)2.2 and Ca(v)2.3. In A-cells, 30 and 15% of I(Ca) is due to Ca(v)2.3 and Ca(v)2.1 activity, respectively, whereas Ca(v)2.2 current was not found in these cells. Low-voltage-activated T-type calcium channels could not be identified in A- and B-cells. Instead, two TTX-sensitive sodium currents were found:…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In ~30% of β-cells an additional Na+ current component is present, accounting for 15-25% of the total Na+ current (~150 pA), with inactivation half-complete at -40 mV, persisting at physiological membrane potentials and capable of contributing to the upstroke of the action potential.
“Not all β-cells conform to this picture, however: in ~30% of β-cells an additional Na+ current component is present, which accounts for 15-25% of the total Na+ current (i.e. ~150 pA) (709, 761). As its inactivation is half-complete at -40 mV, this Na+ current component persists at physiological membrane potentials and is therefore capable of contributing to the upstroke of the action potential (76”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [709]
Cites
Dynamics of Insulin Secretion by the Perfused Rat Pancreas
CURRY, D. L., BENNETT, L. L., & GRODSKY, G. M. (1968) · Endocrinology · doi:10.1210/endo-83-3-572 · Cited by 601 · see citing works
Stimulus-secretion coupling in pancreatic β cells
Ashcroft, F. M., Proks, P., Smith, P. A., Ämmälä, C., Bokvist, K., & Rorsman, P. (1994) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240550007 · Cited by 255 · see citing works
Voltage‐gated and resting membrane currents recorded from B‐cells in intact mouse pancreatic islets
Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., & Rorsman, P. (1999) · The Journal of Physiology · doi:10.1111/j.1469-7793.1999.00717.x · Cited by 226 · see citing works
Tight coupling between electrical activity and exocytosis in mouse glucagon-secreting alpha-cells.
Barg, S., Galvanovskis, J., Göpel, S. O., Rorsman, P., & Eliasson, L. (2000) · Diabetes · doi:10.2337/diabetes.49.9.1500 · Cited by 209 · see citing works
Göpel, S., Zhang, Q., Eliasson, L., Ma, X., Galvanovskis, J., Kanno, T., Salehi, A., & Rorsman, P. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2003.059675 · Cited by 160 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Zamponi, G., Striessnig, J., Koschak, A., & Dolphin, A. (2015) · Pharmacological Reviews · doi:10.1124/pr.114.009654 · Cited by 1,004 · see citing works
“3, in addition to CaV2 channels (Vignali et al., 2006).”
Regulation of Insulin Synthesis and Secretion and Pancreatic Beta-Cell Dysfunction in Diabetes
Fu, Z., Gilbert, E. R., & Liu, D. (2012) · Current Diabetes Reviews · doi:10.2174/157339913804143225 · Cited by 882 · see citing works
“2 of the L-type Ca2+ channels, and P/Q, N and R-type Ca2+ channels were confirmed by single-cell PCR [181].”
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
α-Cells of the Endocrine Pancreas: 35 Years of Research but the Enigma Remains
Gromada, J., Franklin, I., & Wollheim, C. B. (2007) · Endocrine Reviews · doi:10.1210/er.2006-0007 · Cited by 574 · see citing works
MacDonald, P. E., Marinis, Y. Z. D., Ramracheya, R. D., Salehi, A. A., Ma, X., Johnson, P., Cox, R., Eliasson, L., & Rorsman, P. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050143 · Cited by 243 · see citing works
Regulation of calcium in pancreatic α- and β-cells in health and disease
Rorsman, P., Braun, M., & Zhang, Q. (2012) · Cell Calcium · doi:10.1016/j.ceca.2011.11.006 · Cited by 220 · see citing works
Electrophysiology of Islet Cells
Drews, G., Krippeit-Drews, P., & Düfer, M. (2010) · Advances in Experimental Medicine and Biology · doi:10.1007/978-90-481-3271-3_7 · Cited by 152 · see citing works
“3 channel inhibitor SNX 482 account for 30 % of Ca influx in murine alpha cells (Vignali et al. 2006) but seem not to play any role for glucose-regulated glucagon secretion in rat alpha cells (Olsen et al.”
BackgroundInfluentialInsulin as a physiological modulator of glucagon secretion
Bansal, P., & Wang, Q. (2008) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90295.2008 · Cited by 148 · see citing works
“Mouse -cells lack low-voltage-activated Ca(2) channels (89) and possess only L-, P/Q-, N-, and R-type Ca(2) channels (149), Address for reprint requests and other correspondence: Q.”
BackgroundGlucose Suppression of Glucagon Secretion
Marchand, S. L., & Piston, D. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.069195 · Cited by 119 · see citing works
TRPM3 channels provide a regulated influx pathway for zinc in pancreatic beta cells
Wagner, T. F. J., Drews, A., Loch, S., Mohr, F., Philipp, S. E., Lambert, S., & Oberwinkler, J. (2010) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-010-0838-9 · Cited by 92 · see citing works
Zhang, Q., Chibalina, M. V., Bengtsson, M., Groschner, L. N., Ramracheya, R., Rorsman, N. J. G., Leiss, V., Nassar, M. A., Welling, A., Gribble, F. M., et al. (2014) · The Journal of Physiology · doi:10.1113/jphysiol.2014.274209 · Cited by 90 · see citing works
“7 is expressed in both α- and β-cells (Vignali et al. 2006), we observed that Scn9a is quantitatively far more important in β-cells than α-cells depolarisations to membrane potentials between −10 and +20 mV.”
BackgroundSelf-citationIonic mechanisms in pancreatic β cell signaling
Yang, S. N., Shi, Y., Yang, G., Li, Y., Yu, J. H., & Berggren, P. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1680-6 · Cited by 91 · see citing works
“cell accommodates more than 16 ion channel families consisting of about 60 different types of ion channels responsible for complex ionic mechanisms (Table 1) [1, 2, 5–80].”
BackgroundZuccotti, A., Clementi, S., Reinbothe, T., Torrente, A., Vandael, D., & Pirone, A. (2011) · TIPS - Trends in Pharmacological Sciences · doi:10.1016/j.tips.2011.02.012 · Cited by 86 · see citing works
Channel regulation of glucose sensing in the pancreatic beta-cell.
Hiriart, M., & Aguilar-Bryan, L. (2008) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.90493.2008 · Cited by 85 · see citing works
“1 (α1G) are present in INS-1 cells, rat and dog and human (98, 64, 11); but not in mouse (92).”
Reinbothe, T. M., Alkayyali, S., Ahlqvist, E., Tuomi, T., Isomaa, B., Lyssenko, V., & Renström, E. (2013) · Diabetologia · doi:10.1007/s00125-012-2758-z · Cited by 84 · see citing works
“2 [6, 7, 39, 40], with a diminished first phase after ablation of the channel [7].”
BackgroundZhu, L., Almaça, J., Dadi, P., Hong, H., Sakamoto, W., Rossi, M., Lee, R. J., Vierra, N., Lu, H.-Y., Cui, Y.-H., et al. (2017) · Nature Communications · doi:10.1038/ncomms14295 · Cited by 82 · see citing works
Villar-Pazos, S., Martínez‐Pinna, J., Castellano-Muñoz, M., Alonso‐Magdalena, P., Marroquí, L., Quesada, I., Gustafsson, J.-Α., & Nadal, Á. (2017) · Scientific Reports · doi:10.1038/s41598-017-11995-3 · Cited by 91 · see citing works
Quoix, N., Cheng, R., Mattart, L., Zeinoun, Z., Guiot, Y., Beauvois, M. C., Henquin, J., & Gilon, P. (2008) · Diabetes · doi:10.2337/db07-1298 · Cited by 78 · see citing works
Egr-1-A Ca(2+)-regulated transcription factor.
Thiel, G., Mayer, S., Müller, I., Stefano, L., & Rössler, O. G. (2010) · Cell Calcium · doi:10.1016/j.ceca.2010.02.005 · Cited by 67 · see citing works
Cha, C. Y., Nakamura, Y., Himeno, Y., Wang, J., Fujimoto, S., Inagaki, N., Earm, Y. E., & Noma, A. (2011) · The Journal of General Physiology · doi:10.1085/jgp.201110611 · Cited by 61 · see citing works
“In preliminary studies, we implemented INa based on recordings in pancreatic cells from rat (Hiriart and Matteson, 1988), mouse (Göpel et al., 1999b; Vignali et al., 2006), and human (Braun et al., 2008).”
MethodInfluentialUnperturbed islet α‐cell function examined in mouse pancreas tissue slices
Huang, Y., Rupnik, M. S., & Gaisano, H. Y. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.200345 · Cited by 71 · see citing works
“In contrast, the previous study (Vignali et al. 2006) employed single-cell RT-PCR to screen and identify α-cells and then functionally characterize these selected cells.”
BackgroundResultInfluentialXia, F., Leung, Y., Gaisano, G., Gao, X., Chen, Y., Fox, J. E. M., Bhattacharjee, A., Wheeler, M. B., Gaisano, H. Y., & Tsushima, R. G. (2007) · Endocrinology · doi:10.1210/en.2006-1296 · Cited by 54 · see citing works
Functional identification of islet cell types by electrophysiological fingerprinting
Briant, L. J. B., Zhang, Q., Vergari, E., Kellard, J. A., Rodriguez, B., Ashcroft, F. M., & Rorsman, P. (2017) · Journal of The Royal Society Interface · doi:10.1098/rsif.2016.0999 · Cited by 59 · see citing works
Marchand, S. L., & Piston, D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0047084 · Cited by 57 · see citing works
Action potentials and insulin secretion: new insights into the role of Kv channels
Jacobson, D. A., & Philipson, L. H. (2007) · Diabetes, obesity and metabolism · doi:10.1111/j.1463-1326.2007.00784.x · Cited by 55 · see citing works
Blockade of Na+ Channels in Pancreatic α-Cells Has Antidiabetic Effects
Dhalla, A. K., Yang, M., Ning, Y., Kahlig, K. M., Krause, M., Rajamani, S., & Belardinelli, L. (2014) · Diabetes · doi:10.2337/db13-1562 · Cited by 53 · see citing works
“It is known that α-cells express Tetrodotoxin (TTX)-sensitive NaCh isoforms and NaCh blockers like TTX inhibits glucagon secretion (8-10).”
BackgroundResultModeling the pancreatic α-cell: dual mechanisms of glucose suppression of glucagon secretion
Watts, M., & Sherman, A. (2014) · Biophysical Journal · doi:10.1016/j.bpj.2013.11.4504 · Cited by 45 · see citing works
Sodium channel NaV1.3 is important for enterochromaffin cell excitability and serotonin release
Strege, P., Knutson, K. R., Eggers, S. J., Li, J. H., Wang, F., Linden, D., Szurszewski, J., Milescu, L., Leiter, A., Farrugia, G., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-15834-3 · Cited by 45 · see citing works
Quantitative aspects of L-type Ca2+ currents.
Tuckwell, H. (2011) · Progress in neurobiology · doi:10.1016/j.pneurobio.2011.09.010 · Cited by 45 · see citing works
“According to Vignali et al. (2006), 60% of calcium current in both mouse pancreatic A- and B-cells is L-type, with mainly Cav1.”
Vukcevic, M., Spagnoli, G., Iezzi, G., Zorzato, F., & Treves, S. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m804472200 · Cited by 43 · see citing works
Channelopathies, painful neuropathy, and diabetes: which way does the causal arrow point?
Hoeijmakers, J. G. J., Faber, C. G., Merkies, I. S. J., & Waxman, S. G. (2014) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2014.06.003 · Cited by 42 · see citing works
Modulation of Ionic Channels and Insulin Secretion by Drugs and Hormones in Pancreatic Beta Cells
Velasco, M., Díaz‐García, C. M., Larqué, C., & Hiriart, M. (2016) · Molecular Pharmacology · doi:10.1124/mol.116.103861 · Cited by 40 · see citing works
“3 are widely expressed in pancreatic beta cells of rodents (Vignali et al., 2006; Zhang et al., 2014), whereas Nav1.”
BackgroundFKBP12.6 disruption impairs glucose-induced insulin secretion.
Noguchi, N., Yoshikawa, T., Ikeda, T., Takahashi, I., Shervani, N. J., Uruno, A., Yamauchi, A., Nata, K., Takasawa, S., Okamoto, H., et al. (2008) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1016/j.bbrc.2008.04.142 · Cited by 37 · see citing works
Montefusco, F., & Pedersen, M. G. (2015) · The Journal of Physiology · doi:10.1113/jp270777 · Cited by 35 · see citing works
“1997) using ω-conotoxin are in contrast to more recent findings showing that N-type channels are very poorly expressed in alpha-cells, and that ω-conotoxin has unspecific effects also on P/Q-type currents (Vignali et al. 2006; Rorsman et al. 2012, 2014).”
BackgroundResultInfluentialTuluc, P., Theiner, T., Jacobo‐Piqueras, N., & Geisler, S. M. (2021) · Cells · doi:10.3390/cells10082004 · Cited by 30 · see citing works
Cho, J.-H., Chen, L., Kim, M.-H., Chow, R. H.-P., Hille, B., & Koh, D. (2010) · Endocrinology · doi:10.1210/en.2009-0362 · Cited by 31 · see citing works
Perez-Alvarez, A., Hernández-Vivanco, A., Caba-González, J. C., & Albillos, A. (2010) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2010.07089.x · Cited by 30 · see citing works
“Both subtypes are found in the same cells in the brain (Hell et al. 1993), heart atria (Mangoni et al. 2003) or pancreatic islet cells (Vignali et al. 2006).”
BackgroundErnst, S. J., Aguilar-Bryan, L., & Noebels, J. (2009) · Endocrinology · doi:10.1210/en.2008-0991 · Cited by 30 · see citing works
AMPA Receptors Regulate Exocytosis and Insulin Release in Pancreatic β Cells
Wu, Z.-Y., Zhu, L.-J., Zou, N., Bombek, L. K., Shao, C.-Y., Wang, N., Wang, X.-X., Liang, L., Xia, J., Rupnik, M., et al. (2012) · Traffic : the International Journal of Intracellular Transport · doi:10.1111/j.1600-0854.2012.01373.x · Cited by 29 · see citing works
“β cells were easily distinguished from α cells by its electrophysiological properties and insulin-specific expression (3,13).”
BackgroundEarly Development of Electrical Excitability in the Mouse Enteric Nervous System
Hao, M. M., Lomax, A., McKeown, S. J., Reid, C., Young, H. M., & Bornstein, J. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.1426-12.2012 · Cited by 28
Kodera, S., Yoshida, M., Dezaki, K., Yada, T., Murayama, T., Kawakami, M., & Kakei, M. (2013) · Endocrine journal · doi:10.1507/endocrj.ej12-0308 · Cited by 26 · see citing works
High-content screening identifies a role for Na + channels in insulin production
Szabat, M., Modi, H., Ramracheya, R. D., Girbinger, V., Chan, F., Lee, J. T. C., Piske, M., Kamal, S., Yang, Y. H. C., Welling, A., et al. (2015) · Royal Society Open Science · doi:10.1098/rsos.150306 · Cited by 24 · see citing works
“Islets were isolated according to previously published protocols [25].”
MethodSelf-citationCyclic GMP Kinase I Modulates Glucagon Release From Pancreatic α-Cells
Leiss, V., Friebe, A., Welling, A., Hofmann, F. B., & Łukowski, R. (2010) · Diabetes · doi:10.2337/db10-0595 · Cited by 24 · see citing works
“Islets were isolated according to previous studies (29).”
BackgroundMethodSelf-citationSUMO1 enhances cAMP‐dependent exocytosis and glucagon secretion from pancreatic α‐cells
Dai, X.-Q., Spigelman, A., Khan, S., Braun, M., Fox, J. E. M., & Macdonald, P. (2014) · Journal of Physiology · doi:10.1113/jphysiol.2014.274084 · Cited by 23 · see citing works
“Voltage-dependent Na+ (Nav) channels are active at physiological membrane potentials in α-cells, and contribute to the AP upstroke (Göpel et al. 2000; Vignali et al. 2006).”
BackgroundTwo populations of pancreatic islet α-cells displaying distinct Ca2+ channel properties☆
Leung, Y., Ahmed, I., Sheu, L., Tsushima, R., Diamant, N., & Gaisano, H. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.04.066 · Cited by 23 · see citing works
Yang, L.-L., Li, Q.-M., Liu, X., & Liu, S.-G. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17091479 · Cited by 22 · see citing works
Dusaulcy, R., Handgraaf, S., Heddad-Masson, M., Visentin, F., Vesin, C., Reimann, F. M., Gribble, F. M., Philippé, J., & Gosmain, Y. (2015) · Endocrinology · doi:10.1210/en.2015-1725 · Cited by 22 · see citing works
Insulinotropic effect of high potassium concentration beyond plasma membrane depolarization.
Belz, M., Willenborg, M., Görgler, N., Hamada, A., Schumacher, K., & Rustenbeck, I. (2014) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.00362.2013 · Cited by 22 · see citing works
ATP Regulates Sodium Channel Kinetics in Pancreatic Islet Beta Cells
Zou, N., Wu, X., Jin, Y., He, M.-Z., Wang, X.-X., Su, L.-D., Rupnik, M., Wu, Z.-Y., Liang, L., & Shen, Y. (2013) · Journal of Membrane Biology · doi:10.1007/s00232-012-9506-7 · Cited by 20 · see citing works
“Recent experiments demonstrate that residual Na channels are present in b cells and modulate Ca influx-induced action potential (Vignali et al. 2006).”
BackgroundGarcía-Delgado, N., Velasco, M., Sánchez-Soto, C., Díaz-García, C. M., & Hiriart, M. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00040 · Cited by 19
“The main results of this study are summarized as follows (1) the percentage of detection of T-type current increases with the stage of development of beta cell.”
BackgroundResultInfluentialSharma, A., Rahman, G., Gorelik, J., & Bhargava, A. (2023) · Cells · doi:10.3390/cells12030461 · Cited by 18
“2 Brain and Peripheral nervous system [26,32] Heart [28,33] Pancreatic islets and Beta cells [30,34] Kidney and liver [35] Adrenal cortex [36] Cav3.”
BackgroundSarmiento, B. E., Menezes, L. F. S., & Schwartz, E. F. (2019) · Molecules · doi:10.3390/molecules24101846 · Cited by 16 · see citing works
Smith, P. A. (2009) · Endocrinology · doi:10.1210/en.2008-0883 · Cited by 16 · see citing works
Analysis of Calcium Channels by Conditional Mutagenesis
Moosmang, S., Kleppisch, T., Wegener, J. W., Welling, A., & Hofmann, F. B. (2007) · Springer eBooks · doi:10.1007/978-3-540-35109-2_19 · Cited by 18 · see citing works
Self-citationAcreman, S., Ma, J., Denwood, G., Gao, R., Tarasov, A. I., Rorsman, P., & Zhang, Q. (2024) · iScience · doi:10.1016/j.isci.2024.109665 · Cited by 15 · see citing works
“18 It is reasonable to speculate that this is the Ca v 1.3 channel (encoded by Cacna1d ), as it is present in a -cells 17 and can activate at negative membrane potentials.”
InfluentialCaV1.2 and CaV1.3 channel hyperactivation in mouse islet β cells exposed to type 1 diabetic serum
Yang, G., Shi, Y., Yu, J., Li, Y., Yu, L., Welling, A., Hofmann, F., Striessnig, J., Juntti-Berggren, L., Berggren, P., et al. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1737-6 · Cited by 13 · see citing works
“3 channels [13].”
BackgroundInfluentialSelf-citationExtracellular factors and immunosuppressive drugs influencing insulin secretion of murine islets
Auer, V. J., Janas, E., Ninichuk, V., Eppler, E., Weiss, T., Kirchner, S., Otto, A., & Stangl, M. (2012) · Clinical and Experimental Immunology · doi:10.1111/j.1365-2249.2012.04645.x · Cited by 13 · see citing works
A computational systems analysis of factors regulating α cell glucagon secretion
Fridlyand, L. E., & Philipson, L. H. (2012) · Islets · doi:10.4161/isl.22193 · Cited by 12 · see citing works
On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.
Pedersen, M. G. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.06.061 · Cited by 12 · see citing works
“It should be noted that L-type and non-L-type channels have similar inactivation kinetics in mouse β-cells since L-type channel contribute to ∼60 % of both the peak Ca2+-current (46) and the integrated current Q during a 100 ms depolarization (34).”
BackgroundMultiparameter screening reveals a role for Na+ channels in cytokine-induced β-cell death.
Yang, Y., Vilin, Y., Roberge, M., Kurata, H., & Johnson, J. D. (2014) · Molecular Endocrinology · doi:10.1210/me.2013-1257 · Cited by 11
Jackson, M. W., & Gordon, T. (2010) · JIM - Journal of Immunological Methods · doi:10.1016/j.jim.2010.07.005 · Cited by 10
“Furthermore, the effect of the Abs on the secretory activity of beta cells is of interest, as Ltype VGCCs are key regulators of insulin secretion in both human and murine beta cells (Misler et al., 1992; Vignali et al., 2006; Braun et al., 2008; Hiriart and Aguilar-Bryan, 2008).”
BackgroundSeemann, N., Welling, A., & Rustenbeck, I. (2017) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2017.11.024 · Cited by 9 · see citing works
Self-citationGLP-1 Localisation and Proglucagon Gene Expression in Healthy and Diabetic Mouse Ileum
Taşçi, S. K., & Bingöl, S. A. (2018) · Journal of Veterinary Research · doi:10.2478/jvetres-2018-0033 · Cited by 8 · see citing works
The organ-protective effect of N-type Ca(2+) channel blockade.
Kuwahara, K., & Kimura, T. (2015) · Pharmacology and Therapeutics · doi:10.1016/j.pharmthera.2015.02.001 · Cited by 8 · see citing works
Drobinskaya, I., Neumaier, F., Pereverzev, A., Hescheler, J., & Schneider, T. (2015) · Biochimica et Biophysica Acta · doi:10.1016/j.bbamcr.2015.01.001 · Cited by 8
Godazgar, M., Zhang, Q., Chibalina, M. V., & Rorsman, P. (2018) · The Journal of Physiology · doi:10.1113/jp275587 · Cited by 6 · see citing works
“However, more recently it was reported that inactivation in β-cells is biphasic and consists of an additional small Na+ current component (10–15% of the total Na+ current) that persists at physiologically relevant membrane potentials in one-third of the β-cells (Vignali et al. 2006; Zhang et al. 2014).”
BackgroundRegulation of Insulin Granule Exocytosis
Renström, E., & Rorsman, P. (2008) · Springer eBooks · doi:10.1007/978-4-431-75452-7_9 · Cited by 7 · see citing works
Orečná, M., Hafko, R., Toporcerová, V., S̆trbák, V., & Bačová, Z. (2006) · Cellular Physiology and Biochemistry · doi:10.1159/000320543 · Cited by 7
Minimal state models for ionic channels involved in glucagon secretion.
González-Vélez, V., Gil, A., & Quesada, I. (2010) · Mathematical biosciences and engineering : MBE · doi:10.3934/mbe.2010.7.793 · Cited by 6 · see citing works
Physiological and Pathophysiological Control of Glucagon Secretion by Pancreatic α-Cells
Gilon, P., Cheng, R., Lai, B. K., Chae, H., & Gómez-Ruiz, A. (2014) · Springer eBooks · doi:10.1007/978-94-007-6884-0_37-2 · Cited by 8 · see citing works
Voltage-gated calcium channels (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database
Catterall, W., Perez-Reyes, E., Snutch, T., & Striessnig, J. (2019) · IUPHAR/BPS Guide to Pharmacology CITE · doi:10.2218/gtopdb/f80/2019.4 · Cited by 3 · see citing works
The Amount of Releasable Insulin Depends on Continuous Oxidative Phosphorylation
Tappe, C., Parambath, M., Reschke, J., & Rustenbeck, I. (2025) · Function · doi:10.1093/function/zqaf033 · Cited by 2 · see citing works
Voltage-gated calcium channels (CaV) in GtoPdb v.2023.1
Catterall, W., Pérez-Reyes, E., Snutch, T. P., & Striessnig, J. (2023) · IUPHAR/BPS Guide to Pharmacology CITE · doi:10.2218/gtopdb/f80/2023.1 · Cited by 2 · see citing works
Voltage-gated calcium channels (version 2020.5) in the IUPHAR/BPS Guide to Pharmacology Database
Catterall, W., Perez-Reyes, E., Snutch, T., & Striessnig, J. (2020) · IUPHAR/BPS Guide to Pharmacology CITE · doi:10.2218/gtopdb/f80/2020.5 · Cited by 2 · see citing works
Voltage-gated calcium channels in GtoPdb v.2021.2
Catterall, W., Perez-Reyes, E., Snutch, T., & Striessnig, J. (2021) · IUPHAR/BPS Guide to Pharmacology CITE · doi:10.2218/gtopdb/f80/2021.2 · Cited by 2 · see citing works
Ion Channels and Insulin Secretion
Jacobson, D., & Philipson, L. (2008) · Springer eBooks · doi:10.1007/978-4-431-75452-7_6 · Cited by 2 · see citing works
Mathematical models and multiscale simulations of cellular secretion processes
González‐Vélez, V. (2011) · doi:10.5821/dissertation-2117-94768
“Sodium channels activate and inactivate as a function of membrane potential with a threshold voltage in the range of -30 to -20 mV [76, 9, 186].”
BackgroundVoltage-gated calcium channels (CaV) in GtoPdb v.2025.3
Catterall, W., Pérez-Reyes, E., Snutch, T. P., & Striessnig, J. (2025) · IUPHAR/BPS Guide to Pharmacology CITE · doi:10.2218/gtopdb/f80/2025.3
The right stuff: beta-cell channels, cycles, and sensors.
Halban, P. (2008) · American Journal of Physiology. Endocrinology and Metabolism · doi:10.1152/ajpendo.90692.2008
Prevention of type 1 diabetes by carbamazepine in non-obese diabetic mice
Lee, J. T. C. (2017) · doi:10.14288/1.0354479
“Although VGSCs in mice seem to play a relatively minor role, knockout of Scn1b subunit impairs GSIS and results in glucose intolerance (188).”
BackgroundInfluentialIdentification and characterization of pancreatic beta-cell survival factors
Yang, Y. (2014) · doi:10.14288/1.0103408
“7 in mouse islet cells (140), and further indicated that Nav1.”
BackgroundNelson, P. L. (2010) · doi:10.31390/gradschool_theses.3041
“The identification of the non-L-type VDCCs is still unclear but may be T-, N-, R-, or P-types depending on the species and the particular study (Gopel et al., 2000; Gromada et al., 1997; Vignali et al., 2006).”
Background
- Authors
- Vignali, S., Leiss, V., Karl, R., Hofmann, F., & Welling, A.
- Affiliations
- Technical University of Munich (DE)
- ORCID
- Hofmann, Franz
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2006-04-21
- Volume
- 572
- Issue
- 3
- Pages
- 691-706
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1780015
- OpenAlex
- W1574747114
- Cited by
- 96
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 6 · 2016: 2 · 2015: 6 · 2014: 13 · 2013: 4 · 2012: 10
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
- Funders
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Keywords
- voltage-gated sodium channels, voltage-gated calcium channels, pancreatic A cells, pancreatic beta cells, pancreatic islets, insulin secretion, glucagon secretion, L-type calcium channels, Cav1.2, Cav1.3, Cav2.1, CaV2.2, Cav2.3, T-type calcium channels, Nav1.7, single-cell RT-PCR
- MeSH terms
- Animals, Cells, Cultured, Membrane Potentials, Tissue Distribution, Calcium Channels, Sodium Channels, Ion Channel Gating, Glucagon-Secreting Cells, Insulin-Secreting Cells, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
