Multifunctional microbubbles for image-guided antivascular endothelial growth factor therapy
Open accessAbstract
We synthesize multifunctional microbubbles (MBs) for targeted delivery of antivascular endothelial growth factor (antiVEGF) therapy with multimodal imaging guidance. Poly-lactic-co-glycolic acid (PLGA) MBs encapsulating Texas Red dye are fabricated by a modified double-emulsion process. Simultaneous ultrasound and fluorescence imaging are achieved using Texas Red encapsulated MBs. The MBs are conjugated with Avastin, an antiVEGF antibody for treating neovascular age-related macular degeneration (AMD). The conjugation efficiency is characterized by enzyme-linked immunosorbent assay (ELISA). The efficiency for targeted binding of Avastin-conjugated MBs is characterized by microscopic imaging. Our work demonstrates the technical potential of using multifunctional MBs for targeted delivery of antiVEGF therapy in the treatment of exudative AMD.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Multifunctional particles can be formulated from a component material to reduce side effects of the encapsulated drug.
“Such multifunctional particles can also be formulated from a component material to reduce side effects of the encapsulated drug [70].”
From Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier (2011) · cites this paper as [70]
The paper describes multifunctional PLGA microbubbles made from a biocompatible, biodegradable material for targeted delivery (Abstract; page unavailable). It does not report reduced drug side effects; the encapsulated substance in this study was Texas Red dye, while the anti-VEGF drug Avastin was conjugated to the microbubble surface. (LLM, conf=0.87)
Experimental — accuracy not yet measuredThe high rate of clearance of micro/nanoparticles by the reticuloendothelial system and difficulty penetrating many tissue types may limit their use to the vascular space.
“However, the high rate clearance of micro/nanoparticles by the body’s reticuloendothelial system (RES) and the difficultyfor such particles to penetrate many tissue types may limit their use to the vascular space [69–71].”
From Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier (2011) · cites this paper as [70]
The Abstract discusses micro- and nanobubbles for imaging and intraocular drug delivery, but does not address clearance by the reticuloendothelial system or difficulty penetrating tissue. Searches for those mechanisms and for confinement to the vascular space found no relevant passage in our copy. Page numbers were not provided. (LLM, conf=0.75)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Jain, R. A. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00115-0 · Cited by 2,214 · see citing works
Prevalence of age-related maculopathy
Klein, R., Klein, B. E. K., & Linton, K. L. P. (1992) · Ophthalmology · doi:10.1016/s0161-6420(92)31871-8 · Cited by 1,845 · see citing works
Prevalence of age-related maculopathy in Australia
Mitchell, P., Smith, W., Attebo, K., & Wang, J. J. (1995) · Ophthalmology · doi:10.1016/s0161-6420(95)30846-9 · Cited by 1,062 · see citing works
Prevalence of Age-Related Maculopathy in Older Europeans
Augood, C. A. (2006) · Archives of Ophthalmology · doi:10.1001/archopht.124.4.529 · Cited by 387 · see citing works
The Price of Sight — Ranibizumab, Bevacizumab, and the Treatment of Macular Degeneration
Steinbrook, R. (2006) · New England Journal of Medicine · doi:10.1056/nejmp068185 · Cited by 255 · see citing works
Multifunctional microbubbles and nanobubbles for photoacoustic and ultrasound imaging
Kim, C., Qin, R., Xu, J. S., Wang, L. V., & Xu, R. X. (2010) · Journal of Biomedical Optics · doi:10.1117/1.3302808 · Cited by 110 · see citing works
Self-citationXu, J. S., Huang, J., Qin, R., Hinkle, G. H., Povoski, S. P., Martin, E. W., & Xu, R. X. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2009.11.052 · Cited by 108 · see citing works
Self-citationMoshfeghi, A., & Peyman, G. A. (2005) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2005.09.006 · Cited by 97 · see citing works
Use of Microbubbles as an Optical Coherence Tomography Contrast Agent
Barton, J. K., Hoying, J. B., & Sullivan, C. J. (2002) · Academic Radiology · doi:10.1016/s1076-6332(03)80395-1 · Cited by 81 · see citing works
Xu, R. X., Huang, J., Xu, J. S., Sun, D., Hinkle, G. H., Martin, E. W., & Povoski, S. P. (2009) · Journal of Biomedical Optics · doi:10.1117/1.3147424 · Cited by 69 · see citing works
Self-citationHeat-sensitive microbubbles for intraoperative assessment of cancer ablation margins
Huang, J., Xu, J. S., & Xu, R. X. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.11.008 · Cited by 68 · see citing works
Self-citationNeovascular age-related macular degeneration: Present and future treatment options
Votruba, M., & Gregor, Z. J. (2001) · Eye · doi:10.1038/eye.2001.147 · Cited by 59 · see citing works
Age-related macular degeneration: current treatments
Hubschman, J. (2009) · Clinical ophthalmology · doi:10.2147/opth.s2094 · Cited by 53 · see citing works
Age-related macular degeneration: experimental and emerging treatments
Hubschman, J. (2009) · Clinical ophthalmology · doi:10.2147/opth.s5156 · Cited by 16 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier
Makadia, H. K., & Siegel, S. J. (2011) · Polymers · doi:10.3390/polym3031377 · Cited by 4,816 · see citing works
“Such multifunctional particles can also be formulated from a component material to reduce side effects of the encapsulated drug [70].”
BackgroundPu, C., Chang, S., Sun, J., Zhu, S., Liu, H., Zhu, Y., Wang, Z., & Xu, R. X. (2013) · Molecular Pharmaceutics · doi:10.1021/mp400523h · Cited by 75 · see citing works
Self-citationRamajayam, K., Ganesan, S., Ramesh, P., Beena, M., Kokulnathan, T., & Palaniappan, A. (2023) · Biomimetics · doi:10.3390/biomimetics8020245 · Cited by 70 · see citing works
Zhang, Y., Hong, H., Engle, J. W., Yang, Y., Barnhart, T. E., & Cai, W. (2012) · PubMed Central · Cited by 61 · see citing works
Masłowska, K., Halik, P. K., Tymecka, D., Misicka, A., & Gniazdowska, E. (2021) · Cancers · doi:10.3390/cancers13051072 · Cited by 52 · see citing works
Multifunctional microbubbles and nanobubbles for photoacoustic imaging
Xu, R. X. (2011) · Contrast Media & Molecular Imaging · doi:10.1002/cmmi.442 · Cited by 43 · see citing works
Self-citationOptical droplet vaporization of nanoparticle-loaded stimuli-responsive microbubbles
Si, T., Li, G., Wu, Q., Zhu, Z., Luo, X., & Xu, R. X. (2016) · Applied Physics Letters · doi:10.1063/1.4944539 · Cited by 37 · see citing works
Self-citationYe, Z., Ji, Y., Ma, X., Wen, J., Wei, W., & Huang, S. (2015) · Europe PMC (PubMed Central) · doi:10.3980/j.issn.2222-3959.2015.04.02 · Cited by 34 · see citing works
Ultrasound-modulated fluorescence based on fluorescent microbubbles
Liu, Y., Feshitan, J. A., Wei, M., Borden, M. A., & Yuan, B. (2014) · Journal of Biomedical Optics · doi:10.1117/1.jbo.19.8.085005 · Cited by 23 · see citing works
VEGF in nuclear medicine: Clinical application in cancer and future perspectives (Review)
Taurone, S., Galli, F., Signore, A., Agostinelli, E., Dierckx, R., Minni, A., Pucci, M., & Artico, M. (2016) · International Journal of Oncology · doi:10.3892/ijo.2016.3553 · Cited by 19 · see citing works
Drug-loaded biodegradable microspheres for image-guided combinatory epigenetic therapy in cells
Xu, R. X., Xu, J. S., Zuo, T., Huang, T. H. ‐., Rulong, S., & Tweedle, M. F. (2011) · Journal of Biomedical Optics · doi:10.1117/1.3548878 · Cited by 18 · see citing works
Self-citationUltrasound-mediated strategies in opening brain barriers for drug brain delivery
Zhao, Y., Lu, C., Li, X., & Cai, J. (2013) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2013.787987 · Cited by 18 · see citing works
Multifunctionalized Microbubbles for Cancer Diagnosis and Therapy
Li, H., Wang, J., Huang, G., Wang, P., Zheng, R., Zhang, C., & Jiang, Q. Y. (2013) · Anti-Cancer Agents in Medicinal Chemistry · doi:10.2174/187152013804910352 · Cited by 6 · see citing works
Poly(lactic-co-glycolic acid) in drug delivery and tissue engineering applications
Ramesh, P., Ramajayam, K., Beena, M., Ganesan, S., & Palaniappan, A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95233-0.00013-3 · Cited by 3 · see citing works
Prakash Raj, K., Kathiresan, K., & Pandian, P. L. (2021) · Journal of Evolution of Medical and Dental Sciences · doi:10.14260/jemds/2021/416 · Cited by 1 · see citing works
Bu, W., Li, B., Feng, L., Feng, Q., Hu, K., Xu, Z., Wang, Y., Xi, M., Wang, H., Yang, G., et al. (2021) · PubMed Central · Cited by 1 · see citing works
- Authors
- Zhang, L., Xu, J. S., Sanders, V. E., Letson, A. D., Roberts, C. J., & Xu, R. X.
- Affiliations
- The Ohio State University (US)
- Los Alamos National Laboratory (US)
- Venue
- Journal of Biomedical Optics
- Publisher
- SPIE-Intl Soc Optical Eng
- Published
- 2010-05-01
- Volume
- 15
- Issue
- 03
- Pages
- 1-3
- ISSN
- 1083-3668 (ISSN-L)
- 1560-2281
- OpenAlex
- W2136036251
- Cited by
- 16
- References
- 14
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 3 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 1
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Biomedical Optics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
- Physical Sciences › Engineering › Biomedical Engineering › Photoacoustic and Ultrasonic Imaging
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- microbubbles, anti-VEGF therapy, bevacizumab, neovascular age-related macular degeneration, targeted drug delivery, Texas Red, ultrasonography, fluorescence imaging, antibody conjugation, image-guided therapy
- MeSH terms
- Bevacizumab, Antibodies, Monoclonal, Diagnostic Imaging, Enzyme-Linked Immunosorbent Assay, Humans, Macular Degeneration, Microscopy, Electron, Scanning, Logistic Models, Drug Delivery Systems, Angiogenesis Inhibitors, Vascular Endothelial Growth Factor A, Microbubbles, Antibodies, Monoclonal, Humanized
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
