A microneedle device for rapid dermal interstitial fluid sampling
Open accessPublished version of a preprint
Abstract
Dermal interstitial fluid (ISF) offers a promising alternative to invasive blood tests and opportunities for skin diagnostics. Progress in both the understanding and adoption of ISF tests is hindered by sampling challenges, including lengthy collection times, non-negligible failure rates, variable collection volumes, and inconsistent bioanalyte levels. The causes of many of these issues are not well understood. We demonstrate a microneedle device that is several times faster than state of the art, collecting an average of 15.5 mg of ISF in 5 minutes in humans with near-zero failure rate. This improvement was achieved by designing the spatial pressure gradient driving ISF flow. The influence of penetration depth, collection time, pressure, and age on ISF collection was elucidated, with Darcy’s law explaining multiple observations. A data-driven acceptance criterion of <1% blood contamination for ISF is proposed. The device and findings presented will empower researchers to better conduct robust studies in the development of ISF diagnostics.
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- Authors
- Hung, A. H., Kamat, N. U., Bermudez, A., Boczek, S. M., Garcia-Marqués, F. J., Tan, Y. L., Hwang, J. (., Sinawang, P. D., Ilyin, D., Jacobson, G. B., Demirci, U., Poplack, S. P., Pitteri, S. J., & DeSimone, J. M.
- Affiliations
- Stanford Medicine (US)
- Department of Bioengineering, Stanford University, CA 94305, USA.
- Stanford University (US)
- Department of Radiology, School of Medicine, Stanford University, CA 94305, USA.
- Corresponding author
- DeSimone, Joseph M.
- ORCID
- Venue
- Science Advances
- Published
- 2025-09-26
- Volume
- 11
- Issue
- 39
- Pages
- eadx5492-eadx5492
- ISSN
- 2375-2548 (ISSN-L)
- PMC
- PMC12459406
- OpenAlex
- W4414533144
- Cited by
- 22
- References
- 47
- Citations by year
- 2026: 22
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Science Advances (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Funders
- National Cancer Institute (T32CA009695)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Sensor and Energy Harvesting Materials
- Keywords
- dermal interstitial fluid, dermatological diagnosis, Darcy's law, penetration depth, blood contamination, collection time, non-invasive sampling
- MeSH terms
- Humans, Needles, Skin, Specimen Handling, Extracellular Fluid
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