The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Visualization of a Ran-GTP Gradient in Interphase and Mitotic Xenopus Egg Extracts
Abstract
The small guanosine triphosphatase Ran is loaded with guanosine triphosphate (GTP) by the chromatin-bound guanine nucleotide exchange factor RCC1 and releases import cargoes in the nucleus during interphase. In mitosis, Ran-GTP promotes spindle assembly around chromosomes by locally discharging cargoes that regulate microtubule dynamics and organization. We used fluorescence resonance energy transfer–based biosensors to visualize gradients of Ran-GTP and liberated cargoes around chromosomes in mitotic Xenopus egg extracts. Both gradients were required to assemble and maintain spindle structure. During interphase, Ran-GTP was highly enriched in the nucleoplasm, and a steep concentration difference between nuclear and cytoplasmic Ran-GTP was established, providing evidence for a Ran-GTP gradient surrounding chromosomes throughout the cell cycle.
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Citations
Cites
Transport Between the Cell Nucleus and the Cytoplasm
Görlich, D., & Kutay, U. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.607 · Cited by 2,022
Nucleocytoplasmic transport: the soluble phase
Mattaj, I. W., & Englmeier, L. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.265 · Cited by 1,175
Chapter 30 Cell Cycle Extracts
Murray, A. W. (1991) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60298-8 · Cited by 953
Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence Microscopy
Gordon, G. W., Berry, G., Liang, X. H., Levine, B., & Herman, B. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)77976-7 · Cited by 854
Rexach, M., & Blobel, G. (1995) · Cell · doi:10.1016/0092-8674(95)90181-7 · Cited by 763
Ran Induces Spindle Assembly by Reversing the Inhibitory Effect of Importin α on TPX2 Activity
Gruss, O. J., Carazo-Salas, R. E., Schatz, C. A., Guarguaglini, G., Kast, J., Wilm, M., Le Bot, N., Vernos, I., Karsenti, E., & Mattaj, I. W. (2001) · Cell · doi:10.1016/s0092-8674(01)00193-3 · Cited by 593
Structure of importin-β bound to the IBB domain of importin-α
Cingolani, G., Petosa, C., Weis, K., & Müller, C. W. (1999) · Nature · doi:10.1038/20367 · Cited by 576
Self-citationXia, Z., & Liu, Y. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)75886-9 · Cited by 518
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation
Carazo-Salas, R. E., Guarguaglini, G., Gruss, O. J., Segref, A., Karsenti, E., & Mattaj, I. W. (1999) · Nature · doi:10.1038/22133 · Cited by 491
Görlich, D., Henklein, P., Laskey, R. A., & Hartmann, E. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00530.x · Cited by 416
Cited by
Cell-signalling dynamics in time and space
Kholodenko, B. N. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1838 · Cited by 1,347
The Nuclear Pore Complex and Nuclear Transport
Wente, S. R., & Rout, M. P. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000562 · Cited by 686
Regulating Access to the Genome
Weis, K. (2003) · Cell · doi:10.1016/s0092-8674(03)00082-5 · Cited by 674
Self-citationSix Classes of Nuclear Localization Signals Specific to Different Binding Grooves of Importin α
Kosugi, S., Hasebe, M., Matsumura, N., Takashima, H., Miyamoto-Sato, E., Tomita, M., & Yanagawa, H. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m807017200 · Cited by 615
Signalling ballet in space and time
Kholodenko, B. N., Hancock, J. F., & Kolch, W. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2901 · Cited by 614
Metadata
- Authors
- Kalab, P., Weis, K., & Heald, R.
- Venue
- Science
- Published
- 2002-03-29
- Volume
- 295
- Issue
- 5564
- Pages
- 2452-2456
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2029225125
- Cited by
- 530
- References
- 24
- FWCI
- 15.20
- Type
- journal article
- Language
- en
- Open access
- closed
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