The DOI matches its Crossref record.
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
Abstract
We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Super-resolution fluorescence microscopy
Huang, B., Bates, M., & Zhuang, X. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061906.092014 · Cited by 1,657
Applying systems-level spectral imaging and analysis to reveal the organelle interactome
Valm, A. M., Cohen, S., Legant, W. R., Melunis, J., Hershberg, U., Wait, E., Cohen, A. R., Davidson, M. W., Betzig, E., & Lippincott-Schwartz, J. (2017) · Nature · doi:10.1038/nature22369 · Cited by 1,113
Self-citationThe neuronal architecture of the mushroom body provides a logic for associative learning
Aso, Y., Hattori, D., Yu, Y., Johnston, R. M., Iyer, N. A., Ngo, T.-T., Dionne, H., Abbott, L., Axel, R., Tanimoto, H., et al. (2014) · eLife · doi:10.7554/elife.04577 · Cited by 1,051
Principles of two-photon excitation microscopy and its applications to neuroscience
Svoboda, K., & Yasuda, R. (2006) · Neuron · doi:10.1016/j.neuron.2006.05.019 · Cited by 1,027
Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light
Gurskaya, N. G., Verkhusha, V. V., Shcheglov, A. S., Staroverov, D. B., Chepurnykh, T. V., Fradkov, A. F., Lukyanov, S., & Lukyanov, K. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1191 · Cited by 725
Control of local protein synthesis and initial events in myelination by action potentials
Wake, H., Lee, P. R., & Fields, R. D. (2011) · Science · doi:10.1126/science.1206998 · Cited by 636
Mitofusins and OPA1 mediate sequential steps in mitochondrial membrane fusion
Song, Z., Ghochani, M., McCaffery, J. M., Frey, T. G., & Chan, D. C. (2009) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-03-0252 · Cited by 623
Local, persistent activation of Rho GTPases during plasticity of single dendritic spines
Murakoshi, H., Wang, H., & Yasuda, R. (2011) · Nature · doi:10.1038/nature09823 · Cited by 564
Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks
Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., & Nussenzweig, A. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200510015 · Cited by 518
Writing Memories with Light-Addressable Reinforcement Circuitry
Claridge-Chang, A., Roorda, R. D., Vrontou, E., Sjulson, L., Li, H., Hirsh, J., & Miesenböck, G. (2009) · Cell · doi:10.1016/j.cell.2009.08.034 · Cited by 484
The Subspine Organization of Actin Fibers Regulates the Structure and Plasticity of Dendritic Spines
Honkura, N., Matsuzaki, M., Noguchi, J., Ellis-Davies, G. C. R., & Kasai, H. (2008) · Neuron · doi:10.1016/j.neuron.2008.01.013 · Cited by 490
The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine
Harvey, C. D., Yasuda, R., Zhong, H., & Svoboda, K. (2008) · Science · doi:10.1126/science.1159675 · Cited by 434
Karbowski, M., Arnoult, D., Chen, H., Chan, D. C., Smith, C. L., & Youle, R. J. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200309082 · Cited by 433
Brain-wide mapping of neural activity controlling zebrafish exploratory locomotion
Dunn, T. W., Mu, Y., Narayan, S., Randlett, O., Naumann, E. A., Yang, C.-T., Schier, A. F., Freeman, J., Engert, F., & Ahrens, M. B. (2016) · eLife · doi:10.7554/elife.12741 · Cited by 312
“To probe for such an architecture, we used a combination of calcium imaging and photoactivateable GFP (PA-GFP) ( Patterson, 2002 ; Ruta et al., 2010 ; Datta et al., 2008 ).”
Rab6 regulates transport and targeting of exocytotic carriers
Grigoriev, I., Splinter, D., Keijzer, N., Wulf, P. S., Demmers, J., Ohtsuka, T., Modesti, M., Maly, I. V., Grosveld, F., Hoogenraad, C. C., et al. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.010 · Cited by 361
A dimorphic pheromone circuit in Drosophila from sensory input to descending output
Ruta, V., Datta, S. R., Vasconcelos, M. L., Freeland, J., Looger, L. L., & Axel, R. (2010) · Nature · doi:10.1038/nature09554 · Cited by 344
Neuronal activity regulates diffusion across the neck of dendritic spines
Bloodgood, B. L., & Sabatini, B. L. (2005) · Science · doi:10.1126/science.1114816 · Cited by 344
The functional organization of descending sensory-motor pathways in Drosophila
Namiki, S., Dickinson, M. H., Wong, A. M., Korff, W., & Card, G. M. (2018) · eLife · doi:10.7554/elife.34272 · Cited by 337
Chandra, A., Grecco, H. E., Pisupati, V., Perera, D., Cassidy, L., Skoulidis, F., Ismail, S. A., Hedberg, C., Hanzal-Bayer, M., Venkitaraman, A. R., et al. (2012) · Nature Cell Biology · doi:10.1038/ncb2394 · Cited by 325
Transsynaptic mapping of second-order taste neurons in flies by trans-Tango
Talay, M., Richman, E. B., Snell, N. J., Hartmann, G. G., Fisher, J. D., Sorkaç, A., Santoyo, J. F., Chou-Freed, C., Nair, N., Johnson, M., et al. (2017) · Neuron · doi:10.1016/j.neuron.2017.10.011 · Cited by 301
Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons
Cheng, X.-T., Xie, Y.-X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z.-H. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201711083 · Cited by 294
Rapid functional maturation of nascent dendritic spines
Zito, K., Scheuss, V., Knott, G., Hill, T., & Svoboda, K. (2009) · Neuron · doi:10.1016/j.neuron.2008.10.054 · Cited by 272
Steiner, P., Higley, M. J., Xu, W., Czervionke, B. L., Malenka, R. C., & Sabatini, B. L. (2008) · Neuron · doi:10.1016/j.neuron.2008.10.014 · Cited by 259
New Concepts in Synaptic Biology Derived from Single-Molecule Imaging
Triller, A., & Choquet, D. (2008) · Neuron · doi:10.1016/j.neuron.2008.06.022 · Cited by 262
Schmick, M., Vartak, N., Papke, B., Kovacevic, M., Truxius, D. C., Rossmannek, L., & Bastiaens, P. I. H. (2014) · Cell · doi:10.1016/j.cell.2014.02.051 · Cited by 243
The AAA-ATPase p97 is essential for outer mitochondrial membrane protein turnover
Xu, S., Peng, G., Wang, Y., Fang, S., & Karbowski, M. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-09-0748 · Cited by 243
Schellenberg, B., Wang, P., Keeble, J. A., Rodriguez-Enriquez, R., Walker, S., Owens, T. W., Foster, F., Tanianis-Hughes, J., Brennan, K., Streuli, C. H., et al. (2013) · Molecular Cell · doi:10.1016/j.molcel.2012.12.022 · Cited by 237
Regulation of the proteasome by neuronal activity and calcium/calmodulin-dependent protein kinase II
Djakovic, S. N., Schwarz, L. A., Barylko, B., DeMartino, G. N., & Patrick, G. N. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.021956 · Cited by 236
A global view of protein expression in human cells, tissues, and organs
Pontén, F., Gry, M., Fagerberg, L., Lundberg, E., Asplund, A., Berglund, L., Oksvold, P., Björling, E., Hober, S., Kampf, C., et al. (2009) · Molecular Systems Biology · doi:10.1038/msb.2009.93 · Cited by 222
Molecular dissociation of the role of PSD-95 in regulating synaptic strength and LTD.
Xu, W., Schlüter, O. M., Steiner, P., Czervionke, B. L., Sabatini, B., & Malenka, R. C. (2008) · Neuron · doi:10.1016/j.neuron.2007.11.027 · Cited by 193
dal Maschio, M., Donovan, J. C., Helmbrecht, T. O., & Baier, H. (2017) · Neuron · doi:10.1016/j.neuron.2017.04.034 · Cited by 169
Ca2+ and synaptotagmin VII–dependent delivery of lysosomal membrane to nascent phagosomes
Czibener, C., Sherer, N. M., Becker, S. M., Pypaert, M., Hui, E., Chapman, E. R., Mothes, W., & Andrews, N. W. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200605004 · Cited by 165
Francis, A. C., Marin, M., Singh, P. K., Achuthan, V., Prellberg, M. J., Palermino-Rowland, K., Lan, S., Tedbury, P. R., Sarafianos, S. G., Engelman, A. N., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-17256-8 · Cited by 159
Wiring variations that enable and constrain neural computation in a sensory microcircuit
Tobin, W. F., Wilson, R. I., & Lee, W.-C. A. (2017) · eLife · doi:10.7554/elife.24838 · Cited by 123
An Array of Descending Visual Interneurons Encoding Self-Motion in Drosophila
Suver, M. P., Huda, A., Iwasaki, N., Safarik, S., & Dickinson, M. H. (2016) · The Journal of Neuroscience · doi:10.1523/jneurosci.2277-16.2016 · Cited by 110
Histone dynamics in living cells revealed by photobleaching
Kimura, H. (2005) · DNA Repair · doi:10.1016/j.dnarep.2005.04.012 · Cited by 109
Selective photolabeling of proteins using photoactivatable GFP
Patterson, G. H., & Lippincott-Schwartz, J. (2004) · Methods · doi:10.1016/j.ymeth.2003.10.006 · Cited by 107
Self-citationRitter, A. T., Kapnick, S. M., Murugesan, S., Schwartzberg, P. L., Griffiths, G. M., & Lippincott-Schwartz, J. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1710751114 · Cited by 104
Self-citationSingle-molecule spectroscopy and imaging over the decades
Moerner, W. E., Shechtman, Y., & Wang, Q. (2015) · Faraday Discussions · doi:10.1039/c5fd00149h · Cited by 103
Day, C. A., Kraft, L. J., Kang, M., & Kenworthy, A. K. (2012) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy0219s62 · Cited by 89
Neuronal Architecture of a Visual Center that Processes Optic Flow
Kramer, A., Wu, Y., Baier, H., & Kubo, F. (2019) · Neuron · doi:10.1016/j.neuron.2019.04.018 · Cited by 78
Oleinik, N., Kim, J., Roth, B. M., Selvam, S. P., Gooz, M., Johnson, R. H., Lemasters, J. J., & Ogretmen, B. (2019) · Science Advances · doi:10.1126/sciadv.aax1978 · Cited by 68
Noguchi, J., Hayama, T., Watanabe, S., Ucar, H., Yagishita, S., Takahashi, N., & Kasai, H. (2016) · Scientific Reports · doi:10.1038/srep32897 · Cited by 60
AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology
Tilokani, L., Russell, F. M., Hamilton, S., Virga, D. M., Segawa, M., Paupe, V., Gruszczyk, A. V., Protasoni, M., Tabara, L.-C., Johnson, M., et al. (2022) · Science Advances · doi:10.1126/sciadv.abo7956 · Cited by 60
Huttunen, H. J., Peach, C., Bhattacharyya, R., Barren, C., Pettingell, W., Hutter-Paier, B., Windisch, M., Berezovska, O., & Kovacs, D. M. (2009) · The FASEB Journal · doi:10.1096/fj.09-134999 · Cited by 48
Using multifocal plane microscopy to reveal novel trafficking processes in the recycling pathway
Gan, Z., Ram, S., Ober, R. J., & Ward, E. S. (2013) · Journal of Cell Science · doi:10.1242/jcs.116327 · Cited by 38
Fluorescent Protein Applications in Microscopy
Straight, A. F. (2007) · Methods in Cell Biology · doi:10.1016/s0091-679x(06)81006-x · Cited by 24
Metadata
- Authors
- Patterson, G. H., & Lippincott-Schwartz, J.
- Venue
- Science
- Published
- 2002-09-13
- Volume
- 297
- Issue
- 5588
- Pages
- 1873-1877
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1988326309
- Cited by
- 1,612
- References
- 0
- FWCI
- 32.99
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
