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Calorie Restriction Promotes Mitochondrial Biogenesis by Inducing the Expression of eNOS
Abstract
Calorie restriction extends life span in organisms ranging from yeast to mammals. Here, we report that calorie restriction for either 3 or 12 months induced endothelial nitric oxide synthase (eNOS) expression and 3',5'-cyclic guanosine monophosphate formation in various tissues of male mice. This was accompanied by mitochondrial biogenesis, with increased oxygen consumption and adenosine triphosphate production, and an enhanced expression of sirtuin 1. These effects were strongly attenuated in eNOS null-mutant mice. Thus, nitric oxide plays a fundamental role in the processes induced by calorie restriction and may be involved in the extension of life span in mammals.
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“Although the mechanisms behind calorie restriction-driven longevity extension are still poorly understood, mitochondrial physiology is involved in the beneficial effects of calorie restriction in budding yeast11 and Caenorhabditis elegans12, as well as in mammals13.”
From Mitochondrial and metabolic dysfunction in ageing and age-related diseases (2022) · cites this paper as [13]
In the citing paper
“eNOS is induced by CR in mice and human muscle”
From Mitochondrial Activity and Dynamics Changes Regarding Metabolism in Ageing and Obesity (2017) · cites this paper as Nisoli et al., 2005
In the citing paper
“Furthermore, calorie-restriction-induced mitochondrial biogenesis in mice increases PGC-1α, Mfn1 and Mfn2 expression levels in several tissues (Nisoli et al., 2005).”
From New insights into the role of mitochondria in aging: mitochondrial dynamics and more (2010) · cites this paper as Nisoli et al., 2005
In the citing paper
“A number of studies have shown that CR increases mitochondrial biogenesis (Nisoli et al., 2005; Lopez-Lluch et al., 2006)”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Nisoli et al., 2005
In the citing paper
“and mitochondrial efficiency (Nisoli et al., 2005)”
From Mitochondrial (Dys)function and Insulin Resistance: From Pathophysiological Molecular Mechanisms to the Impact of Diet (2019) · cites this paper as Nisoli et al., 2005
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“Calorie restriction is one such established method of improving mitochondrial biogenesis, 232 respiration, 233 and reduction of reactive oxygen species production through upregulation of SIRT1.”
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Herranz, D., Iglesias, G., Muñoz‐Martín, M., & Serrano, M. (2011) · Cell Cycle · doi:10.4161/cc.10.13.16185 · Cited by 19
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“CR is supposed to exert a longevity-promoting effect through enhanced mitochondrial biogenesis, which is initiated by nitric oxide (NO) derived from endothelial nitric oxide synthase (eNOS) (Nisoli et al., 2003; Nisoli et al., 2005; López-Lluch et al., 2006).”
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“Likewise, Sirt1 abundance in several metabolic tissues including the liver, muscle, and brain are increased during caloric restriction (Nisoli et al., 2005; Civitarese et al., 2007; Rodgers et al., 2008).”
BackgroundIs there a role of H2S in mediating health span benefits of caloric restriction?
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Villalba, J. M., Lopez-Dominguez, J. A., Chen, Y., Khraiwesh, H. M., González-Reyes, J. A., Fernández-del-Río, L., Gutiérrez-Casado, E., del Río-Moreno, M., Calvo‐Rubio, M., Ariza, J., et al. (2015) · Biogerontology · doi:10.1007/s10522-015-9572-1 · Cited by 22
“2001; Fontana 2009), and increased mitochondrial biogenesis (López-Lluch et al. 2006; Nisoli et al. 2005; Civitarese et al. 2007) and fatty acid oxidation (Bruss et al.”
InfluentialPerturbation of mitochondrial complex V alters the response to dietary restriction in Drosophila
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“Numerous correlative studies have suggested that alterations in mitochondrial electron transport chain (ETC) function may play a role in mediating the pro-longevity effects of dietary restriction (DR) (Lin et al., 2002; Nisoli et al., 2005; Bishop & Guarente, 2007; Guarente, 2008).”
BackgroundRegulation of Mitochondrial Biogenesis in Metabolic Syndrome
Rolo, A. P., Gomes, A. P., & Palmeira, C. M. (2011) · Current Drug Targets · doi:10.2174/138945011795529056 · Cited by 19
“Upregulation of mitochondrial function in conditions of reduced intake of dietary energy has been proposed has one major mechanism for the beneficial effects of caloric restriction [56].”
BackgroundImpact of Short-Term Hypoxia on Sirtuins as Regulatory Elements in HUVECs
Pecher, S. J., Potthast, A. B., von Versen‐Höynck, F., & Das, A. M. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9082604 · Cited by 17
“, resveratrol) might have a protective effect [19,80,81].”
BackgroundSubcellular Localization Guides eNOS Function
Villadangos, L., & Serrador, J. M. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252413402 · Cited by 21
Mitochondrial efficiency is increased in axenically cultured Caenorhabditis elegans
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Effect of lactate administration on mouse skeletal muscle under calorie restriction
Shirai, T., Uemichi, K., Hidaka, Y., Kitaoka, Y., & Takemasa, T. (2021) · Current Research in Physiology · doi:10.1016/j.crphys.2021.09.001 · Cited by 22
“CR is thought to enhance mitochondrial biogenesis and increase the number of skeletal muscle mitochondria; 30–40% CR in WT mice increased the expression of PGC-1α and mtDNA in the heart (Nisoli et al., 2005).”
BackgroundActein protects against methylglyoxal‐induced oxidative damage in osteoblastic MC3T3‐E1 cells
Suh, K. S., Chon, S., & Choi, E. M. (2016) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.7713 · Cited by 18
Disruption of adenylyl cyclase type 5 mimics exercise training
Guers, J. J., Zhang, J., Campbell, S. C., Oydanich, M. K., Vatner, D. E., & Vatner, S. F. (2017) · Basic Research in Cardiology · doi:10.1007/s00395-017-0648-8 · Cited by 21
“Since NO is linked to the SIRT1 pathway, we assessed its role in mediating the enhanced exercise capacity present in untrained AC5 KO mice and trained WT mice [24, 27, 35].”
BackgroundRegulating aging in adult stem cells with microRNA
Hodzic, M., Naaldijk, Y., & Stolzing, A. (2013) · Zeitschrift für Gerontologie und Geriatrie · doi:10.1007/s00391-013-0531-7 · Cited by 18
Preventing Oxidative Stress in the Liver: An Opportunity for GLP-1 and/or PASK
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“During fasting or calorie restriction, cells are adapted by a metabolic shift in their energy source from glycolysis to oxidative phosphorylation [26–28], which requires an increase in mitochondrial oxidative phosphorylation for producing adenosine triphosphate (ATP), and therefore involves elevated ROS production [29…”
BackgroundWine, sirtuins and nephroprotection: Not only resveratrol
Mannari, C., Bertelli, A. A. E., Stiaccini, G., & Giovannini, L. (2010) · Medical Hypotheses · doi:10.1016/j.mehy.2010.08.004 · Cited by 18
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Calorie restriction: Progress during mid-2005–mid-2006
Anderson, R. M., & Weindruch, R. H. (2006) · Experimental Gerontology · doi:10.1016/j.exger.2006.10.019 · Cited by 18
“A separate study reports increased mitochondrial biogenesis with CR based on increased transcription levels of nuclear encoded mitochondrial genes and increased mitochondrial DNA content in tissues from restricted mice compared to controls (Nisoli et al., 2005).”
BackgroundEpigenetics and Effects on the Neonate That May Impact Feed Efficiency
Meyer, A. M., Caton, J. S. S., Hess, B. W., Ford, S. P., & Reynolds, L. P. (2012) · Wiley eBooks · doi:10.1002/9781118392331.ch13 · Cited by 18
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“demonstrated that SIRT1 expression is induced by increased NO and is blunted in eNOS-deficient mice [43], whereas Mattagajasingh et al.”
BackgroundKetogenesis and SIRT1 as a tool in managing obesity
Abduraman, M. A., Azizan, N. A., Teoh, S. H., & Tan, M. (2020) · Obesity Research & Clinical Practice · doi:10.1016/j.orcp.2020.12.001 · Cited by 19
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“58,59 In contrast to wild-type mice, Kanuri et al. 60 discovered that iNOS knockout mice had significantly increased body weight and fat content but reduced respiratory exchange ratio (RER), volume of carbon dioxide (VCO2), and heat generation.”
MethodDelic, V., Brownlow, M. L., Joly‐Amado, A., Zivkovic, S., Noble, K. V., Phan, T.-A., Ta, Y., Zhang, Y., Bell, S. D., Kurien, C., et al. (2015) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2015.06.001 · Cited by 17
“However, the degree to which CR increases mitochondrial biogenesis remains contested (Cerqueira et al., 2012; Hancock et al., 2011; Nisoli et al., 2005).”
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Mechanisms of endothelial senescence and vascular aging
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“SIRT1 is the most studied SIRT family member for endothelial cell senescence (Nisoli et al. 2005).”
A Critical Assessment of Fasting to Promote Metabolic Health and Longevity
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“To accommodate the shift to fatty acid oxidation, achievement of negative energy balance may promote mitochondrial fission, mitochondrial biogenesis, and improve overall mitochondrial function in part through nutrient-sensitive PGC1 α dependent transcriptional programs (147–149).”
Nahata, M., Fujitsuka, N., Sekine, H., Shimobori, C., Ohbuchi, K., Iizuka, S., Mogami, S., Ohnishi, S., & Takeda, H. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.848960 · Cited by 16
“Moreover, SIRT1 functionally interacts with several transcriptional factors, resulting in enhanced mitochondrial biogenesis (Nisoli et al., 2005; Yuan et al., 2016); thus, it plays an important role in metabolic health, implying that the decline in the metabolic function of the liver of aged FR mice was possibly media…”
BackgroundAlZaim, I., Eid, A. H., Abd‐Elrahman, K. S., & EL‐Yazbi, A. F. (2022) · Biochemical Pharmacology · doi:10.1016/j.bcp.2022.115337 · Cited by 16
Dikalov, S., Gutor, S. S., & Dikalova, A. (2023) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s00424-023-02806-y · Cited by 17
The Roles of White Adipose Tissue and Liver NADPH in Dietary Restriction-Induced Longevity
Jamerson, L. E., & Bradshaw, P. C. (2024) · Antioxidants · doi:10.3390/antiox13070820 · Cited by 22
Restricción calórica, estrés oxidativo y longevidad
Lopez‐Torres, M., & Barja, G. (2008) · Revista Española de Geriatría y Gerontología · doi:10.1016/s0211-139x(08)71190-9 · Cited by 16
“…oxidativo y la longevidad máxima (MLSP) en la eficacia bioenergética mitocondrial 72,73 , descensos en los valores celulares de ROS 73 y ausencia de cambios, o incluso aumentos de consumo de oxígeno 72,74-76 en mo-delos de RC en distintas especies, tales como mamíferos, nematodos y levaduras.”
Milton‐Laskibar, I., Aguirre, L., Fernández‐Quintela, A., Rolo, A. P., Teodoro, J. S., Palmeira, C. M., & Portillo, M. P. (2017) · Nutrients · doi:10.3390/nu9070737 · Cited by 16
“[61] reported that a 30% calorie restriction on mice for three months resulted in greatly increased liver mitochondria, evidenced by increases in the proteins cytochrome c and cytochrome oxidase subunit IV, and the mRNA levels of PGC-1α, among others.”
BackgroundCardiovascular Disease and Aging
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Nitric Oxide Production and Regulation in the Teleost Cardiovascular System
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Goyary, D., & Sharma, R. (2007) · Biogerontology · doi:10.1007/s10522-007-9112-8 · Cited by 16
“It is consistent with the earlier findings that calorie restriction induces mitochondrial biogenesis and expression of genes crucial for dynamic processes required for mitochondrial functions (Nisoli et al. 2005).”
InfluentialMitochondrial activation in the growth-restricted fetus of monochorionic twins
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“Moreover, similarity between the beneficial effects of elevated concentration of circulating adiponectin and of food restriction on some obesity-related diseases [1–3, 29–32], suggest that adiponectin could be a possible functional link between food restriction and the reduction of incidence of some obesity-related di…”
BackgroundSuwa, M., Nakano, H., Radak, Z. O., & Kumagai, S. (2015) · Europe PMC (PubMed Central) · Cited by 16
Harnessing migraines for neural regeneration
Borkum, J. M. (2018) · Neural Regeneration Research · doi:10.4103/1673-5374.230275 · Cited by 16
“…brain under ischemic conditions through a number of mechanisms including vasodilation, antagonism of oxidant production by the renin-angiotensin system (González et al., 2014), induction of antioxidant enzymes (Astort et al., 2014), and stimulation of mitochondrial biogenesis (Nisoli et al., 2005).”
BackgroundLebel, M., Picard, F., Ferland, G., & Gaudreau, P. (2011) · The Journals of Gerontology Series A · doi:10.1093/gerona/glr038 · Cited by 15
Pardo, R., Velilla, M., Herrero, L., Cervela‐Cardona, L., Ribeiro, M. L., Simó, R., & Villena, J. A. (2021) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202000672 · Cited by 15
Impact of metabolic stress induced by diets, aging and fasting on tissue oxygen consumption
Mackert, O., Wirth, E. K., Sun, R., Winkler, J. L., Liu, A., Renko, K., Kunz, S., Spranger, J., & Brachs, S. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101563 · Cited by 17
Senoinflammation as the underlying mechanism of aging and its modulation by calorie restriction
Noh, S. G., Kim, H., Kim, S., Chung, K. W., Jung, Y.-S., Yoon, J.-H., Yu, B. P., Lee, J., & Chung, H. Y. (2024) · Ageing Research Reviews · doi:10.1016/j.arr.2024.102503 · Cited by 16
Keller, A. C., Knaub, L. A., McClatchey, P. M., Connon, C. A., Bouchard, R. J., Miller, M. W., Geary, K. E., Walker, L. A., Klemm, D. J., & Reusch, J. E. B. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/8524267 · Cited by 14
The impact of ageing on lipid-mediated regulation of adult stem cell behavior and tissue homeostasis
Demarco, R. S., Clémot, M., & Jones, D. L. (2020) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2020.111278 · Cited by 16
“Furthermore, CR elevates nitric oxide levels, which stimulates mitochondrial biogenesis and enhances FAO and lipolysis (Nisoli et al., 2005).”
BackgroundWhytock, K. L., Corbin, K. D., Parsons, S. A., Pachori, A. S., Bock, C. P., Jones, K. P., Smith, J. S., Yi, F., Xie, H., Petucci, C. J., et al. (2021) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/nqab027 · Cited by 19
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“showed that SIRT1 is reduced by DR in mouse livers [32] but the others reported that it is increased by DR in rats [33] and mice [34].”
BackgroundShinmura, K. (2013) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2012.08.006 · Cited by 15
“In this regard, nitric oxide derived from eNOS is essential for SIRT1 activity in CR-treated hearts (Nisoli et al. 2005; Shinmura 2010).”
BackgroundPotential of Cydonia oblonga leaves in cardiovascular disease.
Vaez, H., Hamidi, S., & Arami, S. (2014) · Hypothesis · doi:10.5779/hypothesis.v12i1.356 · Cited by 15
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Evolution of Human Longevity Uncoupled from Caloric Restriction Mechanisms
Zhao, G., Guo, S., Somel, M., & Khaitovich, P. (2014) · PLoS ONE · doi:10.1371/journal.pone.0084117 · Cited by 15
“Module 5 genes were enriched in the GO term ‘‘mitochondrion’’, the site of oxidative metabolism in eukaryotes, which is closely linked with aging and CR response [23,65,66].”
BackgroundSIRT-associated attenuation of cellular senescence in vascular wall
Sazdova, I., Hadzi‐Petrushev, N., Keremidarska‐Markova, M., Stojchevski, R., Sopi, R., Shileiko, S. A., Mitrokhin, V. M., Gagov, H., Avtanski, D. B., Lubomirov, L. T., et al. (2024) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2024.111943 · Cited by 16
SIRT-ain relief from age-inducing stress
Zhang, D., Liu, Y., & Chen, D. (2011) · Aging · doi:10.18632/aging.100283 · Cited by 14
“Increasing evidence suggests that metabolic rate does not decrease during CR, and in some cases, even increases [11,12].”
Keep the fire burning: Current avenues in the quest of treating mitochondrial disorders
Tischner, C., & Wenz, T. (2015) · Mitochondrion · doi:10.1016/j.mito.2015.06.002 · Cited by 14
Oxidative Stress in the Aging Process: Fundamental Aspects and New Insights
del Valle, L. G., Hernández, R. G., Roche, L. D., & Fernández, O. (2015) · ACS symposium series · doi:10.1021/bk-2015-1200.ch006 · Cited by 15
Mitochondrial metabolism in aging: Effect of dietary interventions
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Zhong, Q., Li, X., Nong, Q., Mao, B., & Pan, X. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18091905 · Cited by 14
“Notably, previous studies in vivo have shown an eNOS-dependent mitochondrial biogenesis and function in caloric restriction or exercise training models [40,41].”
BackgroundFree Radicals Cardiovascular Diseases: An Update
Bhattacharya, S., Ahmed, K. M., & Chakraborty, S. (2011) · Free Radicals and Antioxidants · doi:10.5530/ax.2011.1.4 · Cited by 14
Supplementation with Essential Amino Acids in Middle Age Maintains the Health of Rat Kidney
Corsetti, G., Stacchiotti, A., D’Antona, G., Nisoli, E., Dioguardi, F. S., & Rezzani, R. (2010) · International Journal of Immunopathology and Pharmacology · doi:10.1177/039463201002300214 · Cited by 14
Oxidative phosphorylation and fatty acid oxidation in slow-aging mice
Elmansi, A. M., & Miller, R. A. (2024) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2024.08.018 · Cited by 14
Sirtuin1 in vascular endothelial function, an overview
Kumar, J., & Kumar, S. (2021) · Epigenetics · doi:10.1080/15592294.2021.1975936 · Cited by 14
“This corroborated an earlier report showing that mice with a genetic deletion of e-NOS exhibit diminished effects of caloric restriction on the increase in Sirtuin1 expression in white adipose tissue [59].”
BackgroundRahman, M. A., Rahman, M. H., Rhim, H., & Kim, B. (2024) · Current Neuropharmacology · doi:10.2174/1570159x22666240426091311 · Cited by 14
“Positive effects of CR were observed at the mitochondrial level, where it was found to influence mitochondrial biogenesis via activation of NO synthase (eNOS) [134].”
BackgroundEstey, C., Seifert, E. L., Aguer, C., Moffat, C., & Harper, M. (2012) · Experimental Gerontology · doi:10.1016/j.exger.2012.02.008 · Cited by 13
“More generally, CR has been associated with remodelling of the mitochondrial mass (Barazzoni et al., 2005; Bevilacqua et al., 2004, 2005; Civitarese et al., 2007; Marzetti et al., 2008; Nisoli et al., 2005) (Asami et al.”
BackgroundResultCalorie restriction up-regulates iron and coppertransportgenes in Saccharomyces cerevisiae
Sharma, P. K., Mittal, N., Deswal, S., & Roy, N. (2010) · Molecular BioSystems · doi:10.1039/c0mb00084a · Cited by 13
Nijhawan, P., & Behl, T. (2019) · Obesity Medicine · doi:10.1016/j.obmed.2019.100156 · Cited by 13
Keeping the beat against time: Mitochondrial fitness in the aging heart
Mendoza, A., & Karch, J. M. (2022) · Frontiers in Aging · doi:10.3389/fragi.2022.951417 · Cited by 13
“While earlier studies have shown that CR can promote mitochondrial biogenesis in healthy and young human skeletal muscle (Nisoli et al., 2005; Civitarese et al., 2007), other reports have observed no connection between CR and mitochondrial biogenesis within the mammalian heart (Hancock et al.”
BackgroundSirtuins in Aging and Age-Related Diseases
Haigis, M. C., & Sinclair, D. A. (2010) · Elsevier eBooks · doi:10.1016/b978-0-12-378638-8.00011-7 · Cited by 13
Abiri, B., & Vafa, M. (2019) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-25650-0_7 · Cited by 13
Increased nitric oxide production in eating disorders
Vannacci, A., Ravaldi, C., Giannini, L., Rotella, C. M., Masini, E., Faravelli, C., & Ricca, V. (2006) · Neuroscience Letters · doi:10.1016/j.neulet.2006.01.060 · Cited by 13
Mittal, N., Babu, M. M., & Roy, N. (2009) · Aging Cell · doi:10.1111/j.1474-9726.2009.00518.x · Cited by 13
“The same observation (low ROS and higher respiration rate) have also been made in multicellular eukaryotes such as C. elegans (Bishop & Guarente, 2007b) and mice (Nisoli et al., 2005).”
InfluentialMitochondrial dysfunction in depression: Mechanisms and targeted therapy strategies
Xue, X., Li, K., Jiang, B., Zou, W., & Wang, L. (2025) · Asian Journal of Psychiatry · doi:10.1016/j.ajp.2025.104694 · Cited by 15
Elmansi, A. M., & Miller, R. A. (2023) · GeroScience · doi:10.1007/s11357-023-00849-8 · Cited by 13
“Consistent with these findings, PGC-1α was reported in multiple studies to be upregulated in liver and adipose tissue of CR mice, and in liver of Snell Dwarf mice [34–38].”
ResultSestrin2 is a central regulator of mitochondrial stress responses in disease and aging
Machado, I. F., Palmeira, C. M., & Rolo, A. P. (2025) · Ageing Research Reviews · doi:10.1016/j.arr.2025.102762 · Cited by 12
Bang, E., Lee, B., Noh, S., Kim, D. H., Jung, H. J., Ha, S., Yu, B. P., & Chung, H. Y. (2019) · BMB Reports · doi:10.5483/bmbrep.2019.52.1.301 · Cited by 12
“It has been shown that CR can upregulate SIRT expression in liver, adipose tissue, brain, and kidney (42) and exert its anti-aging activity via interactions with FoxO1, FoxO3, FoxO4, PGC1, p53, liver X receptor, and NF-B.”
BackgroundSagayama, H., Yoshimura, E., Yamada, Y., & Tanaka, H. (2019) · The Journal of Sports Medicine and Physical Fitness · doi:10.23736/s0022-4707.18.08677-2 · Cited by 12
Longevity. The allostatic load of dietary restriction
Pijl, H. (2011) · Physiology & Behavior · doi:10.1016/j.physbeh.2011.05.030 · Cited by 12
Sánchez-Román, I., Gómez, J., Naudí, A., Ayala, V., Portero‐Otín, M., Lopez‐Torres, M., Pamplona, R., & Barja, G. (2010) · Rejuvenation Research · doi:10.1089/rej.2010.1062 · Cited by 12
Tahara, E. B., Cezário, K., de Souza-Pinto, N. C., de Barros, M. H., & Kowaltowski, A. J. (2011) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-011-9377-0 · Cited by 12
“For example, CR has been shown to increase maximal respiratory capacity in rodents by promoting mitochondrial biogenesis (Nisoli et al. 2005; López-Lluch et al. 2006; Cerqueira et al. 2011), an effect also observed in other animal models with enhanced lifespans such as fat-specific insulin knockout…”
BackgroundProtective effects of honokiol against methylglyoxal-induced osteoblast damage
Suh, K. S., Chon, S., & Choi, E. M. (2015) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2015.12.015 · Cited by 12
Cai, J., Luo, L.-M., Tang, H., & Zhou, L. (2018) · Molecular Biology · doi:10.1134/s0026893318040040 · Cited by 12
Park, K.-A., Jin, Z. H., An, H. S., Lee, J. Y., Jeong, E. A., Choi, E. B., Kim, K. E., Shin, H. J., Lee, J. E., & Roh, G. S. (2019) · Korean Journal of Physiology and Pharmacology · doi:10.4196/kjpp.2019.23.5.335 · Cited by 12
D’Aquila, P., Rango, F. D., Guarasci, F., Mandalà, M., Corsonello, A., Bellizzi, D., & Passarino, G. (2020) · Nutrients · doi:10.3390/nu12020460 · Cited by 12
Rahbarghazi, A., Siahkouhian, M., Rahbarghazi, R., Ahmadi, M., Bolboli, L., Mahdipour, M., Haghighi, L., Hassanpour, M., Nasimi, F. S., & Keyhanmanesh, . (2021) · Tissue and Cell · doi:10.1016/j.tice.2021.101486 · Cited by 12
Nitric oxide: emerging concepts about its use in cell-based therapies
Brunelli, S., Rovere, P. Q., Sciorati, C., Manfredi, A. A., & Clementi, E. (2006) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.16.1.33 · Cited by 13
“An example of this is the ability of NO to trigger mitochondrial biogenesis, a process that is involved in cell differentiation; for example, in the maturation of adipose tissue, which occurs through a coordinated activation of transcription factors triggering expression of both nuclearand mitochondrial-encoded protei…”
BackgroundSalamon, A., Maszlag-Török, R., Veres, G., Boros, F. A., Vágvölgyi-Sümegi, E., Somogyi, A., Vécsei, L., Klivènyi, P., & Zádori, D. (2020) · Neurochemical Research · doi:10.1007/s11064-020-03069-0 · Cited by 11
Celiac ganglia neurolysis suppresses high blood pressure in rats
Dai, S., Zhao, L., Wang, G., Chen, C., Li, C., Xiao, B., & Miao, Y. (2023) · Hypertension Research · doi:10.1038/s41440-023-01305-y · Cited by 11
Özbek, E., Şimşek, A., Özbek, M., & Somay, A. (2013) · Archivio Italiano di Urologia e Andrologia · doi:10.4081/aiua.2013.3.113 · Cited by 11
Metadata
- Authors
- Nisoli, E., Tonello, C., Cardile, A., Cozzi, V., Bracale, R., Tedesco, L., Falcone, S., Valerio, A., Cantoni, O., Clementi, E., Moncada, S., & Carruba, M. O.
- Affiliations
- University of Milan (IT)
- Corresponding author
- Nisoli, Enzo
- ORCID
- Venue
- Science
- Published
- 2005-10-14
- Volume
- 310
- Issue
- 5746
- Pages
- 314-317
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2162306102
- Cited by
- 1,107
- References
- 26
- Citations by year
- 2026: 11 · 2025: 23 · 2024: 28 · 2023: 36 · 2022: 32 · 2021: 44 · 2020: 48 · 2019: 46 · 2018: 37 · 2017: 49 · 2016: 42 · 2015: 70 · 2014: 72 · 2013: 83 · 2012: 103
- FWCI
- 15.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Institutional Research Information System (Università degli Studi di Brescia) (submittedVersion)
- Archivio Istituzionale della Ricerca - Università degli Studi del Molise (submittedVersion)
- CINECA IRIS Institutional Research information system (University of Urbino) (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- caloric restriction, mitochondrial biogenesis, endothelial nitric oxide synthase, nitric oxide, SIRT1, cyclic GMP, lifespan extension, oxygen consumption, ATP production
- MeSH terms
- Adipose Tissue, Animals, DNA, Mitochondrial, Enzyme Induction, Female, Life Expectancy, Male, Mice, Inbred C57BL, Mitochondria, Nitric Oxide, Oxygen Consumption, Protein Biosynthesis, Nitric Oxide Synthase, GTP Phosphohydrolases, Caloric Restriction, Sirtuins, Mice, Nitric Oxide Synthase Type II, Nitric Oxide Synthase Type III, Sirtuin 1
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