The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Graded Regulation of the Kv2.1 Potassium Channel by Variable Phosphorylation
Abstract
Dynamic modulation of ion channels by phosphorylation underlies neuronal plasticity. The Kv2.1 potassium channel is highly phosphorylated in resting mammalian neurons. Activity-dependent Kv2.1 dephosphorylation by calcineurin induces graded hyperpolarizing shifts in voltage-dependent activation, causing suppression of neuronal excitability. Mass spectrometry–SILAC (stable isotope labeling with amino acids in cell culture) identified 16 Kv2.1 phosphorylation sites, of which 7 were dephosphorylated by calcineurin. Mutation of individual calcineurin-regulated sites to alanine produced incremental shifts mimicking dephosphorylation, whereas mutation to aspartate yielded equivalent resistance to calcineurin. Mutations at multiple sites were additive, showing that variable phosphorylation of Kv2.1 at a large number of sites allows graded activity-dependent regulation of channel gating and neuronal firing properties.
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Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome
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A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling
Blagoev, B., Kratchmarova, I., Ong, S.-E., Nielsen, M., Foster, L. J., & Mann, M. (2003) · Nature Biotechnology · doi:10.1038/nbt790 · Cited by 689
Mechanism of Divergent Growth Factor Effects in Mesenchymal Stem Cell Differentiation
Kratchmarova, I., Blagoev, B., Haack-Sorensen, M., Kassem, M., & Mann, M. (2005) · Science · doi:10.1126/science.1107627 · Cited by 565
Protein Phosphorylation and Neuronal Function
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Regulation of ion channel localization and phosphorylation by neuronal activity
Misonou, H., Mohapatra, D. P., Park, E. W., Leung, V., Zhen, D., Misonou, K., Anderson, A. E., & Trimmer, J. S. (2004) · Nature Neuroscience · doi:10.1038/nn1260 · Cited by 463
Self-citationMurakoshi, H., & Trimmer, J. S. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-05-01728.1999 · Cited by 301
Self-citationVariable Control of Ets-1 DNA Binding by Multiple Phosphates in an Unstructured Region
Pufall, M. A., Lee, G. M., Nelson, M. L., Kang, H.-S., Velyvis, A., Kay, L. E., McIntosh, L. P., & Graves, B. J. (2005) · Science · doi:10.1126/science.1111915 · Cited by 246
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Kv2.1: A Voltage-Gated K+ Channel Critical to Dynamic Control of Neuronal Excitability
Misonou, H., Mohapatra, D. P., & Trimmer, J. S. (2005) · NeuroToxicology · doi:10.1016/j.neuro.2005.02.003 · Cited by 210
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Functional and quantitative proteomics using SILAC
Mann, M. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2067 · Cited by 931
Prabakaran, S., Lippens, G., Steen, H., & Gunawardena, J. (2012) · WIREs Systems Biology and Medicine · doi:10.1002/wsbm.1185 · Cited by 335
Phosphorylation in protein-protein binding: effect on stability and function
Nishi, H., Hashimoto, K., & Panchenko, A. R. (2011) · Structure · doi:10.1016/j.str.2011.09.021 · Cited by 322
Johnston, J., Forsythe, I. D., & Kopp-Scheinpflug, C. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.191973 · Cited by 290
Multisite protein phosphorylation – from molecular mechanisms to kinetic models
Salazar, C., & Höfer, T. (2009) · The FEBS Journal · doi:10.1111/j.1742-4658.2009.07027.x · Cited by 258
Metadata
- Authors
- Park, K.-S., Mohapatra, D. P., Misonou, H., & Trimmer, J. S.
- Venue
- Science
- Published
- 2006-08-18
- Volume
- 313
- Issue
- 5789
- Pages
- 976-979
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2063256813
- Cited by
- 262
- References
- 17
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- closed
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