The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Receptor Activation Alters Inner Surface Potential During Phagocytosis
Abstract
The surface potential of biological membranes varies according to their lipid composition. We devised genetically encoded probes to assess surface potential in intact cells. These probes revealed marked, localized alterations in the charge of the inner surface of the plasma membrane of macrophages during the course of phagocytosis. Hydrolysis of phosphoinositides and displacement of phosphatidylserine accounted for the change in surface potential at the phagosomal cup. Signaling molecules such as K-Ras, Rac1, and c-Src that are targeted to the membrane by electrostatic interactions were rapidly released from membrane subdomains where the surface charge was altered by lipid remodeling during phagocytosis.
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“In addition to stage-specific v-, t-SNAREs and Rab proteins, phosphatidylinositide (PI) lipids synthesized by stage-specific phosphatidyl inositol kinases (PIK) at the cytoplasmic sheet of phagosomal membranes are specific for different phagosome stages and functionally contribute to maturation-associated vesicle fusion and actin-polymerization associated processes (16).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [16]
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Self-citationPhosphoinositide Involvement in Phagocytosis and Phagosome Maturation
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- R. J. Botelho, C. C. Scott, S. Grinstein, Curr. Top. Microbiol. Immunol.282, 1 (2004).
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Metadata
- Authors
- Yeung, T., Terebiznik, M., Yu, L., Silvius, J., Abidi, W. M., Philips, M., Levine, T., Kapus, A., & Grinstein, S.
- Affiliations
- University of Toronto (CA)
- Division of Cell Biology, Hepatology, and Nutrition Department, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
- Corresponding author
- Grinstein, Sergio
- ORCID
- Venue
- Science
- Published
- 2006-07-21
- Volume
- 313
- Issue
- 5785
- Pages
- 347-351
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1999972891
- Cited by
- 329
- References
- 9
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 16 · 2023: 15 · 2022: 10 · 2021: 15 · 2020: 17 · 2019: 8 · 2018: 8 · 2017: 11 · 2016: 9 · 2015: 15 · 2014: 13 · 2013: 17 · 2012: 22
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Keywords
- membrane surface potential, phagocytosis, macrophages, plasma membrane, phosphoinositide hydrolysis, phosphatidylserine exposure, lipid remodeling, genetically encoded probes, KRAS, Rac1, Src kinase, lipid rafts, receptor activation
- MeSH terms
- Animals, Calcium, Cell Line, Cell Membrane, Fluorescent Dyes, Immunoglobulin G, Lipid Bilayers, Liposomes, Macrophages, Membrane Potentials, Neuropeptides, Opsonin Proteins, Peptides, Phagocytosis, Phagosomes, Phospholipids, Receptors, Fc, Molecular Probes, Ionomycin, ras Proteins, rac GTP-Binding Proteins, rac1 GTP-Binding Protein, Mice, Static Electricity, Hydrophobic and Hydrophilic Interactions
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