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A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila
Abstract
In Drosophila , repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AGO3. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
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Cited by
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Non-coding RNAs in human disease
Esteller, M. (2011) · Nature Reviews Genetics · doi:10.1038/nrg3074 · Cited by 4,825
Biogenesis of small RNAs in animals
Kim, V. N., Han, J., & Siomi, M. C. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2632 · Cited by 2,814
Self-citationBushati, N., & Cohen, S. M. (2007) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.23.090506.123406 · Cited by 2,750
PIWI-interacting small RNAs: the vanguard of genome defence
Siomi, M. C., Sato, K., Pezic, D., & Aravin, A. A. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3089 · Cited by 1,319
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Metadata
- Authors
- Gunawardane, L. S., Saito, K., Nishida, K. M., Miyoshi, K., Kawamura, Y., Nagami, T., Siomi, H., & Siomi, M. C.
- Venue
- Science
- Published
- 2007-03-16
- Volume
- 315
- Issue
- 5818
- Pages
- 1587-1590
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1971549481
- Cited by
- 1,171
- References
- 0
- FWCI
- 87.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gunawardane, L. S., Saito, K., Nishida, K. M., Miyoshi, K., Kawamura, Y., Nagami, T., Siomi, H., & Siomi, M. C. (2007). A Slicer-Mediated Mechanism for Repeat-Associated siRNA 5' End Formation in Drosophila. Science. https://doi.org/10.1126/science.1140494
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