Parallels Between Cytokinesis and Retroviral Budding: A Role for the ESCRT Machinery
Open accessAbstract
During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two proteins involved in HIV-1 budding—tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein—were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.
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Retroviral budding and cytokinesis culminate with a topologically similar membrane abscission process.
“Both processes of retroviral budding and cytokinesis culminate with a topologically similar membrane abscission process that separates progeny virions from host cell membranes and daughter cells from one another after cell division [13,14,15].”
From Budding of a Retrovirus: Some Assemblies Required (2020) · cites this paper as [14]
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- Authors
- Carlton, J. G., & Martin-Serrano, J.
- Affiliations
- Department of Infectious Diseases, King's College London School of Medicine, London, UK.
- Venue
- Science
- Published
- 2007-06-29
- Volume
- 316
- Issue
- 5833
- Pages
- 1908-1912
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1992752531
- Cited by
- 764
- References
- 2
- Citations by year
- 2026: 21 · 2025: 24 · 2024: 27 · 2023: 47 · 2022: 26 · 2021: 30 · 2020: 32 · 2019: 37 · 2018: 38 · 2017: 38 · 2016: 35 · 2015: 42 · 2014: 30 · 2013: 37 · 2012: 61
- FWCI
- 23.45
- Type
- journal article
- Language
- en
- Open access
- green
- zenodo.org
- Licence
- other-oa
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Keywords
- cytokinesis, retroviral budding, HIV-1 budding, ESCRT machinery, ESCRT-I, TSG101, Alix, abscission, midbody, membrane fission, CEP55, centrosome, cell division
- MeSH terms
- Tsg101 Protein, Adenosine Triphosphatases, Calcium-Binding Proteins, Carrier Proteins, DNA-Binding Proteins, HeLa Cells, Humans, Microtubules, Nuclear Proteins, Protein Binding, Endosomes, Recombinant Fusion Proteins, Transcription Factors, Transfection, HIV-1, Protein Structure, Tertiary, Cell Cycle Proteins, Amino Acid Motifs, Vesicular Transport Proteins, RNA Interference, Cytokinesis, R-SNARE Proteins, Syntaxin 1, Endosomal Sorting Complexes Required for Transport
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