The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F
Open accessAbstract
MicroRNAs (miRNAs) play an important role in gene regulatory networks in animals. Yet, the mechanistic details of their function in translation inhibition or messenger RNA (mRNA) destabilization remain controversial. To directly examine the earliest events in this process, we have developed an in vitro translation system using mouse Krebs-2 ascites cell–free extract that exhibits an authentic miRNA response. We show here that translation initiation, specifically the 5′ cap recognition process, is repressed by endogenous let-7 miRNAs within the first 15 minutes of mRNA exposure to the extract when no destabilization of the transcript is observed. Our results indicate that inhibition of translation initiation is the earliest molecular event effected by miRNAs. Other mechanisms, such as mRNA degradation, may subsequently consolidate mRNA silencing.
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Citations
Cites
The functions of animal microRNAs
Ambros, V. (2004) · Nature · doi:10.1038/nature02871 · Cited by 10,728
Olsen, P. H., & Ambros, V. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9523 · Cited by 1,234
Kahvejian, A., Svitkin, Y. V., Sukarieh, R., M'Boutchou, M.-N., & Sonenberg, N. (2005) · Genes & Development · doi:10.1101/gad.1262905 · Cited by 491
Self-citation
Cited by
(2008) · doi:10.1038/nrg2290 · Cited by 5,199
Widespread changes in protein synthesis induced by microRNAs
Selbach, M., Schwanhäusser, B., Thierfelder, N., Fang, Z., Khanin, R., & Rajewsky, N. (2008) · Nature · doi:10.1038/nature07228 · Cited by 3,429
Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets
Sonenberg, N., & Hinnebusch, A. G. (2009) · Cell · doi:10.1016/j.cell.2009.01.042 · Cited by 3,304
“In contrast to the disparate results obtained from studies in cells, in vitro experiments in cell-free translation extracts prepared from mammalian or Drosophila embryos all indicated that miRNAs suppress cap-dependent but not IRES-mediated translation (Mathonnet et al., 2007; Thermann and Hentze, 2007; Wakiyama et al…”
Self-citationRegulation of mRNA Translation and Stability by microRNAs
Fabian, M. R., Sonenberg, N., & Filipowicz, W. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060308-103103 · Cited by 3,214
Self-citationThe mechanism of eukaryotic translation initiation and principles of its regulation
Jackson, R. J., Hellen, C. U. T., & Pestova, T. V. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2838 · Cited by 2,759
Metadata
- Authors
- Mathonnet, G., Fabian, M. R., Svitkin, Y. V., Parsyan, A., Huck, L., Murata, T., Biffo, S., Merrick, W. C., Darzynkiewicz, E., Pillai, R. S., Filipowicz, W., Duchaine, T. F., & Sonenberg, N.
- Venue
- Science
- Published
- 2007-09-21
- Volume
- 317
- Issue
- 5845
- Pages
- 1764-1767
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2159638567
- Cited by
- 534
- References
- 3
- FWCI
- 17.36
- Type
- journal article
- Language
- en
- Open access
- green
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- Mathonnet, G., Fabian, M. R., Svitkin, Y. V., Parsyan, A., Huck, L., Murata, T., Biffo, S., Merrick, W. C., Darzynkiewicz, E., Pillai, R. S., Filipowicz, W., Duchaine, T. F., & Sonenberg, N. (2007). MicroRNA Inhibition of Translation Initiation in Vitro by Targeting the Cap-Binding Complex eIF4F. Science. https://doi.org/10.1126/science.1146067
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