The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Conformational Switch of Syntaxin-1 Controls Synaptic Vesicle Fusion
Open accessAbstract
During synaptic vesicle fusion, the soluble N -ethylmaleimide-sensitive factor–attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a “closed” conformation outside the SNARE complex and an “open” conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B Open mice were viable but succumbed to generalized seizures at 2 to 3 months of age. Binding of Munc18-1 to syntaxin-1 was impaired in syntaxin-1B Open synapses, and the size of the readily releasable vesicle pool was decreased; however, the rate of synaptic vesicle fusion was dramatically enhanced. Thus, the closed conformation of syntaxin-1 gates the initiation of the synaptic vesicle fusion reaction, which is then mediated by SNARE-complex/Munc18-1 assemblies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The MUN domain may act to open the ‘closed’ form of the SNARE protein syntaxin-1 (Gerber et al., 2008), thereby enabling syntaxin-1 to form SNARE complexes (Richmond et al., 2001; Ma et al., 2011).”
From The Presynaptic Active Zone (2012) · cites this paper as Gerber et al., 2008
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
SNAREs — engines for membrane fusion
Jahn, R., & Scheller, R. H. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2002 · Cited by 2,635
Südhof, T. C. (2004) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.26.041002.131412 · Cited by 2,491
Self-citationCrystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
Sutton, R. B., Fasshauer, D., Jahn, R., & Brunger, A. T. (1998) · Nature · doi:10.1038/26412 · Cited by 2,417
Synaptic assembly of the brain in the absence of neurotransmitter secretion.
Verhage, M., Maia, A., Plomp, J., Brussaard, A., Heeroma, J., Vermeer, H., Toonen, R., Hammer, R., van den Berg, T. K., Missler, M., et al. (2000) · Science · doi:10.1126/science.287.5454.864 · Cited by 1,387
Self-citationDefinition of the readily releasable pool of vesicles at hippocampal synapses.
Rosenmund, C., & Stevens, C. (1996) · Neuron · doi:10.1016/s0896-6273(00)80146-4 · Cited by 1,084
Self-citationThree-dimensional structure of the neuronal-Sec1–syntaxin 1a complex
Misura, K. M. S., Scheller, R. H., & Weis, W. I. (2000) · Nature · doi:10.1038/35006120 · Cited by 790
A conformational switch in syntaxin during exocytosis: role of munc18
Dulubova, I., Sugita, S., Hill, S., Hosaka, M., Fernández, I., Südhof, T., & Rizo, J. (1999) · EMBO Journal · doi:10.1093/emboj/18.16.4372 · Cited by 772
Self-citationSelective Activation of Cognate SNAREpins by Sec1/Munc18 Proteins
Shen, J., Tareste, D. C., Paumet, F., Rothman, J. E., & Melia, T. J. (2007) · Cell · doi:10.1016/j.cell.2006.12.016 · Cited by 487
Functional impact of syntaxin on gating of N-type and Q-type calcium channels
Bezprozvanny, I., Scheller, R., & Tsien, R. (1995) · Nature · doi:10.1038/378623a0 · Cited by 441
An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming
Richmond, J. E., Weimer, R. M., & Jorgensen, E. M. (2001) · Nature · doi:10.1038/35085583 · Cited by 437
Munc18-1 Promotes Large Dense-Core Vesicle Docking
Voets, T., Toonen, R. F., Brian, E. C., de Wit, H., Moser, T., Rettig, J., Südhof, T. C., Neher, E., & Verhage, M. (2001) · Neuron · doi:10.1016/s0896-6273(01)00391-9 · Cited by 396
Self-citationCalcium-dependent interaction of N-type calcium channels with the synaptic core complex
Sheng, Z.-H., Rettig, J., Cook, T., & Catterall, W. (1996) · Nature · doi:10.1038/379451a0 · Cited by 363
Munc18-1 binds directly to the neuronal SNARE complex
Dulubova, I., Khvotchev, M. V., Liu, S., Huryeva, I., Südhof, T. C., & Rizo, J. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0611318104 · Cited by 348
Self-citationStructure and function of SNARE and SNARE-interacting proteins
Brünger, A. T. (2005) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583505004051 · Cited by 308
Defects in synaptic vesicle docking in unc-18 mutants
Weimer, R. M., Richmond, J. E., Davis, W. S., Hadwiger, G., Nonet, M. L., & Jørgensen, E. M. (2003) · Nature Neuroscience · doi:10.1038/nn1118 · Cited by 262
Regulation of CFTR chloride channels by syntaxin and Munc18 isoforms
Naren, A. P., Nelson, D. J., Xie, W., Jovov, B., Pevsner, J., Bennett, M. K., Benos, D. J., Quick, M. W., & Kirk, K. L. (1997) · Nature · doi:10.1038/36882 · Cited by 217
Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+‐triggered neurotransmitter release
Pang, Z. P., Sun, J., Rizo, J., Maximov, A., & Südhof, T. C. (2006) · The EMBO Journal · doi:10.1038/sj.emboj.7601103 · Cited by 174
Self-citationKhvotchev, M. V., Dulubova, I., Sun, J., Dai, H., Rizo, J., & Südhof, T. C. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.3655-07.2007 · Cited by 163
Self-citationTransport rates of GABA transporters: regulation by the N-terminal domain and syntaxin 1A
Deken, S. L., Beckman, M. L., Boos, L., & Quick, M. W. (2000) · Nature Neuroscience · doi:10.1038/79939 · Cited by 158
Docking of Secretory Vesicles Is Syntaxin Dependent
de Wit, H., Cornelisse, L. N., Toonen, R. F. G., & Verhage, M. (2006) · PLoS ONE · doi:10.1371/journal.pone.0000126 · Cited by 130
Self-citationPhosphorylated Syntaxin 1 Is Localized to Discrete Domains Along a Subset of Axons
Foletti, D. L., Lin, R. C.-S., Finley, M., & Scheller, R. H. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-12-04535.2000 · Cited by 88
Fluorescence relaxation spectroscopy in the analysis of macromolecular structure and motion
Rigler, R. W., & Ehrenberg, M. (1976) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500002122 · Cited by 60
Syntaxin 1A Regulates ENaC Channel Activity
Condliffe, S. B., Zhang, H., & Frizzell, R. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313592200 · Cited by 55
Research works printed but not linked: 1
- A. T. Brunger, Q. Rev. Biophys.9, 1 (2005).
Cited by
Sorted by citations
Membrane Fusion: Grappling with SNARE and SM Proteins
Südhof, T. C., & Rothman, J. E. (2009) · Science · doi:10.1126/science.1161748 · Cited by 2,027
Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle
Südhof, T. C. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.022 · Cited by 1,197
Südhof, T. C. (2012) · Neuron · doi:10.1016/j.neuron.2012.06.012 · Cited by 1,062
Molecular machines governing exocytosis of synaptic vesicles
Jahn, R., & Fasshauer, D. (2012) · Nature · doi:10.1038/nature11320 · Cited by 1,004
Synaptic Vesicle ExocytosisPreprint
Südhof, T. C., & Rizo, J. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005637 · Cited by 539
Self-citation
Metadata
- Authors
- Gerber, S. H., Rah, J.-C., Min, S.-W., Liu, X., de Wit, H., Dulubova, I., Meyer, A. C., Rizo, J., Arancillo, M., Hammer, R. E., Verhage, M., Rosenmund, C., & Südhof, T. C.
- Affiliations
- Baylor College of Medicine (US)
- Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390–9111, USA.
- Corresponding author
- Gerber, Stefan H.
- Rah, Jong-Cheol
- Min, Sang-Won
- Rosenmund, Christian
- ORCID
- Venue
- Science
- Published
- 2008-09-12
- Volume
- 321
- Issue
- 5895
- Pages
- 1507-1510
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3235364
- OpenAlex
- W2006897184
- Cited by
- 294
- References
- 23
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 7 · 2023: 15 · 2022: 9 · 2021: 13 · 2020: 16 · 2019: 12 · 2018: 15 · 2017: 15 · 2016: 15 · 2015: 18 · 2014: 28 · 2013: 22 · 2012: 24
- FWCI
- 10.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Pure Amsterdam UMC (submittedVersion)
- VU Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of Neurological Disorders and Stroke (NS 37200, R01NS037200, R01NS051262, NS051262)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- syntaxin-1, Munc18-1, SNARE complex, synaptic vesicle fusion, conformational switching, exocytosis, seizures, synaptic transmission
- MeSH terms
- Animals, Calcium, Epilepsy, Membrane Fusion, Mutation, Protein Conformation, Sucrose, Synapses, Synaptic Vesicles, Protein Structure, Tertiary, Mice, Knockout, Excitatory Postsynaptic Potentials, SNARE Proteins, Syntaxin 1, Mice, Munc18 Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
