The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Conformational Switch of Syntaxin-1 Controls Synaptic Vesicle Fusion
Open accessAbstract
During synaptic vesicle fusion, the soluble N -ethylmaleimide-sensitive factor–attachment protein receptor (SNARE) protein syntaxin-1 exhibits two conformations that both bind to Munc18-1: a “closed” conformation outside the SNARE complex and an “open” conformation in the SNARE complex. Although SNARE complexes containing open syntaxin-1 and Munc18-1 are essential for exocytosis, the function of closed syntaxin-1 is unknown. We generated knockin/knockout mice that expressed only open syntaxin-1B. Syntaxin-1B Open mice were viable but succumbed to generalized seizures at 2 to 3 months of age. Binding of Munc18-1 to syntaxin-1 was impaired in syntaxin-1B Open synapses, and the size of the readily releasable vesicle pool was decreased; however, the rate of synaptic vesicle fusion was dramatically enhanced. Thus, the closed conformation of syntaxin-1 gates the initiation of the synaptic vesicle fusion reaction, which is then mediated by SNARE-complex/Munc18-1 assemblies.
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“The MUN domain may act to open the ‘closed’ form of the SNARE protein syntaxin-1 (Gerber et al., 2008), thereby enabling syntaxin-1 to form SNARE complexes (Richmond et al., 2001; Ma et al., 2011).”
From The Presynaptic Active Zone (2012) · cites this paper as Gerber et al., 2008
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Citations
Cites
SNAREs — engines for membrane fusion
Jahn, R., & Scheller, R. H. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2002 · Cited by 2,635
Südhof, T. C. (2004) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.26.041002.131412 · Cited by 2,491
Self-citationCrystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
Sutton, R. B., Fasshauer, D., Jahn, R., & Brunger, A. T. (1998) · Nature · doi:10.1038/26412 · Cited by 2,417
Synaptic assembly of the brain in the absence of neurotransmitter secretion.
Verhage, M., Maia, A., Plomp, J., Brussaard, A., Heeroma, J., Vermeer, H., Toonen, R., Hammer, R., van den Berg, T. K., Missler, M., et al. (2000) · Science · doi:10.1126/science.287.5454.864 · Cited by 1,387
Self-citationDefinition of the readily releasable pool of vesicles at hippocampal synapses.
Rosenmund, C., & Stevens, C. (1996) · Neuron · doi:10.1016/s0896-6273(00)80146-4 · Cited by 1,084
Self-citationThree-dimensional structure of the neuronal-Sec1–syntaxin 1a complex
Misura, K. M. S., Scheller, R. H., & Weis, W. I. (2000) · Nature · doi:10.1038/35006120 · Cited by 790
A conformational switch in syntaxin during exocytosis: role of munc18
Dulubova, I., Sugita, S., Hill, S., Hosaka, M., Fernández, I., Südhof, T., & Rizo, J. (1999) · EMBO Journal · doi:10.1093/emboj/18.16.4372 · Cited by 772
Self-citationSelective Activation of Cognate SNAREpins by Sec1/Munc18 Proteins
Shen, J., Tareste, D. C., Paumet, F., Rothman, J. E., & Melia, T. J. (2007) · Cell · doi:10.1016/j.cell.2006.12.016 · Cited by 487
Functional impact of syntaxin on gating of N-type and Q-type calcium channels
Bezprozvanny, I., Scheller, R., & Tsien, R. (1995) · Nature · doi:10.1038/378623a0 · Cited by 441
An open form of syntaxin bypasses the requirement for UNC-13 in vesicle priming
Richmond, J. E., Weimer, R. M., & Jorgensen, E. M. (2001) · Nature · doi:10.1038/35085583 · Cited by 437
Research works printed but not linked: 1
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Cited by
Sorted by citations
Membrane Fusion: Grappling with SNARE and SM Proteins
Südhof, T. C., & Rothman, J. E. (2009) · Science · doi:10.1126/science.1161748 · Cited by 2,027
Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle
Südhof, T. C. (2013) · Neuron · doi:10.1016/j.neuron.2013.10.022 · Cited by 1,197
Südhof, T. C. (2012) · Neuron · doi:10.1016/j.neuron.2012.06.012 · Cited by 1,062
Molecular machines governing exocytosis of synaptic vesicles
Jahn, R., & Fasshauer, D. (2012) · Nature · doi:10.1038/nature11320 · Cited by 1,004
Synaptic Vesicle ExocytosisPreprint
Südhof, T. C., & Rizo, J. (2011) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005637 · Cited by 539
Self-citation
Metadata
- Authors
- Gerber, S. H., Rah, J.-C., Min, S.-W., Liu, X., de Wit, H., Dulubova, I., Meyer, A. C., Rizo, J., Arancillo, M., Hammer, R. E., Verhage, M., Rosenmund, C., & Südhof, T. C.
- Affiliations
- Baylor College of Medicine (US)
- Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390–9111, USA.
- Corresponding author
- Gerber, Stefan H.
- Rah, Jong-Cheol
- Min, Sang-Won
- Rosenmund, Christian
- ORCID
- Venue
- Science
- Published
- 2008-09-12
- Volume
- 321
- Issue
- 5895
- Pages
- 1507-1510
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3235364
- OpenAlex
- W2006897184
- Cited by
- 294
- References
- 23
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 7 · 2023: 15 · 2022: 9 · 2021: 13 · 2020: 16 · 2019: 12 · 2018: 15 · 2017: 15 · 2016: 15 · 2015: 18 · 2014: 28 · 2013: 22 · 2012: 24
- FWCI
- 10.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Pure Amsterdam UMC (submittedVersion)
- VU Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of Neurological Disorders and Stroke (NS 37200, R01NS037200, R01NS051262, NS051262)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- syntaxin-1, Munc18-1, SNARE complex, synaptic vesicle fusion, conformational switching, exocytosis, seizures, synaptic transmission
- MeSH terms
- Animals, Calcium, Epilepsy, Membrane Fusion, Mutation, Protein Conformation, Sucrose, Synapses, Synaptic Vesicles, Protein Structure, Tertiary, Mice, Knockout, Excitatory Postsynaptic Potentials, SNARE Proteins, Syntaxin 1, Mice, Munc18 Proteins
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