The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Phasic Firing in Dopaminergic Neurons Is Sufficient for Behavioral Conditioning
Open accessAbstract
Rewarding Bursts of Dopamine: Dopaminergic neurons are thought to be involved in the cognitive and hedonic underpinnings of motivated behaviors. However, it is still unclear whether dopaminergic neuron activation is sufficient to elicit reward-related behavior and which type of neuronal activity pattern serves this purpose. Tsai et al. (p. 1080; published online 23 April) directly compared tonic versus phasic firing of dopaminergic cells in the ventral tegmental area, and the effects on both behavior and dopamine release. Using a transgenic system and virus injection in mice, they targeted the dopaminergic cells with rhodopsin. Light stimulation was then used to drive dopaminergic cells either with a tonic low level of pulses or bursts of high-frequency pulses, with the number of pulses being equal across conditions. Only the high-frequency phasic firing induced a conditioned place preference and dopamine release.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“There is an increase in DA release (measured in awake, behaving animals by microdialysis or by fastscanning cyclic voltammetry) in the NAc in response to unexpected food rewards or stimuli that predict food rewards [72,308-310].”
From Reward circuitry dysfunction in psychiatric and neurodevelopmental disorders and genetic syndromes: animal models and clinical findings (2012) · cites this paper as [72]
In the citing paper
“This evidence was recently further investigated in an animal study using optogenetics, which showed that activation of the VTA leads to dopamine release in the NAc (Tsai et al., 2009; for a review, see Lenz and Lobo, 2013).”
From Circuit Mechanisms of Reward, Anhedonia, and Depression (2019) · cites this paper as Tsai et al., 2009
In the citing paper
“However, since optogenetic activation of VTA DA neurons increases social interaction (Gunaydin et al., 2014) and supports positive reinforcement (Tsai et al., 2009, Witten et al., 2011), they are thought to play a causal role in social reward.”
From Dorsal raphe dopamine neurons represent the experience of social isolation (2016) · cites this paper as Tsai et al., 2009
In the citing paper
“The light activated channelrhodopsin (ChR2) (19, 20) has been used in other neural systems to activate specific cell populations and produce learning (21–23).”
From Optical activation of lateral amygdala pyramidal cells instructs associative fear learning (2010) · cites this paper as [21]
In the citing paper
“conversely, simulating burst firing using channelrhodopsin is sufficient to induce behavioral conditioning [42].”
From A neoHebbian framework for episodic memory; role of dopamine-dependent late LTP (2011) · cites this paper as [42]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The neural basis of drug craving: An incentive-sensitization theory of addiction
Robinson, T. (1993) · Brain Research Reviews · doi:10.1016/0165-0173(93)90013-p · Cited by 7,346
Millisecond-timescale, genetically targeted optical control of neural activity
Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. A. (2005) · Nature Neuroscience · doi:10.1038/nn1525 · Cited by 5,015
Self-citationNeural systems of reinforcement for drug addiction: from actions to habits to compulsion
Everitt, B. J., & Robbins, T. W. (2005) · Nature Neuroscience · doi:10.1038/nn1579 · Cited by 3,785
Dopamine, learning and motivation.
Wise, R. A. (2004) · Nature Reviews Neuroscience · doi:10.1038/nrn1406 · Cited by 3,379
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel
Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold, P. R., Ollig, D., Hegemann, P., & Bamberg, E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1936192100 · Cited by 2,730
Drug abuse: hedonic homeostatic dysregulation
Koob, G. F., & Moal, M. L. (1997) · Science · doi:10.1126/science.278.5335.52 · Cited by 2,535
The mesolimbic dopamine reward circuit in depression
Nestler, E. J., & Carlezon, W. A. (2006) · Biological Psychiatry · doi:10.1016/j.biopsych.2005.09.018 · Cited by 2,059
Dopamine neuron systems in the brain: An update
Björklund, A., & Dunnett, S. B. (2007) · Trends in Neurosciences · doi:10.1016/j.tins.2007.03.006 · Cited by 1,777
Multimodal fast optical interrogation of neural circuitry
Zhang, F., Wang, L., Brauner, M., Liewald, J. F., Kay, K., Watzke, N., Wood, P. G., Bamberg, E., Nagel, G., GOTTSCHALK, A. R., et al. (2007) · Nature · doi:10.1038/nature05744 · Cited by 1,766
Self-citationHabits, Rituals, and the Evaluative Brain
Graybiel, A. M. (2008) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.29.051605.112851 · Cited by 1,697
Cited by
Sorted by citations
Dopamine in motivational control: rewarding, aversive, and alerting
Bromberg-Martin, E. S., Matsumoto, M., & Hikosaka, O. (2010) · Neuron · doi:10.1016/j.neuron.2010.11.022 · Cited by 2,396
Deisseroth, K. A. (2010) · Nature Methods · doi:10.1038/nmeth.f.324 · Cited by 1,994
Self-citationOptogenetics in neural systems
Yizhar, O., Fenno, L. E., Davidson, T. J., Mogri, M., & Deisseroth, K. (2011) · Neuron · doi:10.1016/j.neuron.2011.06.004 · Cited by 1,881
Self-citationThe brain reward circuitry in mood disorders
Russo, S. J., & Nestler, E. J. (2013) · Nature Reviews Neuroscience · doi:10.1038/nrn3381 · Cited by 1,870
The development and application of optogenetics
Fenno, L., Yizhar, O., & Deisseroth, K. (2011) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-061010-113817 · Cited by 1,822
Self-citation
Metadata
- Authors
- Tsai, H.-C., Zhang, F., Adamantidis, A., Stuber, G. D., Bonci, A., de Lecea, L., & Deisseroth, K.
- Affiliations
- Stanford University (US)
- University of California, San Francisco (US)
- Department of Psychiatry and Behavioral Sciences, W083 Clark Center, 318 Campus Drive West, Stanford University, Stanford, CA 94305, USA.
- Corresponding author
- Tsai, Hsing-Chen
- Zhang, Feng
- Deisseroth, Karl
- ORCID
- Venue
- Science
- Published
- 2009-05-22
- Volume
- 324
- Issue
- 5930
- Pages
- 1080-1084
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC5262197
- OpenAlex
- W1980737345
- Cited by
- 1,266
- References
- 28
- Citations by year
- 2026: 31 · 2025: 53 · 2024: 63 · 2023: 51 · 2022: 68 · 2021: 58 · 2020: 74 · 2019: 68 · 2018: 74 · 2017: 92 · 2016: 90 · 2015: 99 · 2014: 105 · 2013: 87 · 2012: 82
- FWCI
- 34.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Bern Open Repository and Information System (University of Bern) (submittedVersion)
- Funders
- National Institute of Mental Health (R01MH087592)
- National Institute on Drug Abuse (R01DA021880)
- National Institute of Neurological Disorders and Stroke (K99NS065009)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural and Behavioral Psychology Studies
- Keywords
- phasic firing, dopaminergic neurons, ventral tegmental area, behavioral conditioning, optogenetic stimulation, natural reward, drugs of abuse
- MeSH terms
- Channelrhodopsins, Action Potentials, Animals, Behavior, Animal, Conditioning, Psychological, Dopamine, Electrodes, Implanted, Genetic Vectors, Light, Mice, Transgenic, Neurons, Reward, Transduction, Genetic, Ventral Tegmental Area, Patch-Clamp Techniques, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
