Cited by 247Nuclear Structure and FunctionPluripotent Stem Cells ResearchDevelopmental Biology and Gene Regulation

Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells

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Kim, Youngjo, Sharov, Alexei A., McDole, Katie, Cheng, Melody, Hao, Haiping, Fan, Chen-Ming, Gaiano, Nicholas, Ko, Minoru S. H. +1 more·2011·Science

Abstract

B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.

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  1. [15]Peric-Hupkes D Mol. Cell 2010 38 603 20513434 PMC5975946

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Authors
Kim, Y., Sharov, A. A., McDole, K., Cheng, M., Hao, H., Fan, C.-M., Gaiano, N., Ko, M. S. H., & Zheng, Y.
Affiliations
  • Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA.
  • National Institutes of Health (US)
  • Howard Hughes Medical Institute (US)
  • Johns Hopkins University (US)
Corresponding author
  • Ko, Minoru S. H.
  • Zheng, Yixian
Venue
Science
Published
2011-12-23
Volume
334
Issue
6063
Pages
1706-1710
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2088969742
Cited by
247
References
35
Citations by year
2026: 9 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 8 · 2020: 19 · 2019: 13 · 2018: 18 · 2017: 8 · 2016: 15 · 2015: 27 · 2014: 29 · 2013: 36 · 2012: 35
FWCI
13.13
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01 GM 56312, R01GM056312)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR060042)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
B-type lamins, lamin B, nuclear lamina, organogenesis, mouse embryonic stem cells, self-renewal, pluripotency, gene silencing, trophectoderm, neural progenitor cells, spindle orientation, neuronal migration
MeSH terms
Animals, Brain, Cell Cycle, Cell Differentiation, Cell Movement, Cells, Cultured, Chromatin, Female, Male, Spindle Apparatus, Neurons, Organ Size, Promoter Regions, Genetic, Transcription, Genetic, Trophoblasts, Mice, Knockout, Gene Expression Regulation, Developmental, Gene Silencing, Nuclear Lamina, Lamin Type B, Organogenesis, Pluripotent Stem Cells, Embryonic Development, Body Size, Mice, Embryonic Stem Cells, Neural Stem Cells

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