Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells
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B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.
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The Lamin B family of proteins is required for proper organogenesis and cell survival.
“This family of proteins is required to a proper organogenesis and cell survival (42).”
From Senolytic Combination of Dasatinib and Quercetin Alleviates Intestinal Senescence and Inflammation and Modulates the Gut Microbiome in Aged Mice (2021) · cites this paper as [42]
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Cites
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Mikkelsen, T. S., Ku, M., Jaffe, D. B., Issac, B., Lieberman, E., Giannoukos, G., Alvarez, P., Brockman, W., Kim, T.-K., Koche, R. P., et al. (2007) · Nature · doi:10.1038/nature06008 · Cited by 4,129 · see citing works
Niwa, H., Miyazaki, J., & Smith, A. G. (2000) · Nature Genetics · doi:10.1038/74199 · Cited by 3,904 · see citing works
Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
Guelen, L., Pagie, L., Brasset, É., Meuleman, W. R. A., Faza, M. B., Talhout, W., Eussen, B. H. J., de Klein, A., Wessels, L. F. A., de Laat, W. L., et al. (2008) · Nature · doi:10.1038/nature06947 · Cited by 1,959 · see citing works
Interaction between Oct3/4 and Cdx2 Determines Trophectoderm Differentiation
Niwa, H., Toyooka, Y., Shimosato, D., Strumpf, D., Takahashi, K., Yagi, R., & Rossant, J. (2005) · Cell · doi:10.1016/j.cell.2005.08.040 · Cited by 1,192 · see citing works
Molecular Maps of the Reorganization of Genome-Nuclear Lamina Interactions during Differentiation
Peric-Hupkes, D., Meuleman, W., Pagie, L., Bruggeman, S. W. M., Solovei, I., Brugman, W., Gräf, S., Flicek, P., Kerkhoven, R. M., van Lohuizen, M., et al. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.03.016 · Cited by 1,050 · see citing works
Stem cell transcriptome profiling via massive-scale mRNA sequencing
Cloonan, N., Forrest, A. R. R., Kolle, G., Gardiner, B. B. A., Faulkner, G. J., Brown, M. K., Taylor, D. F., Steptoe, A. L., Wani, S., Bethel, G., et al. (2008) · Nature Methods · doi:10.1038/nmeth.1223 · Cited by 1,035 · see citing works
Dechat, T., Pfleghaar, K., Sengupta, K., Shimi, T., Shumaker, D. K., Solimando, L., & Goldman, R. D. (2008) · Genes & Development · doi:10.1101/gad.1652708 · Cited by 1,001 · see citing works
Transcriptional repression mediated by repositioning of genes to the nuclear lamina
Reddy, K. L., Zullo, J., Bertolino, E., & Singh, H. (2008) · Nature · doi:10.1038/nature06727 · Cited by 785 · see citing works
Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
Lanctôt, C., Cheutin, T., Cremer, M., Cavalli, G., & Cremer, T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2041 · Cited by 781 · see citing works
Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo
Nichols, J., Silva, J., Roode, M., & Smith, A. G. (2009) · Development · doi:10.1242/dev.038893 · Cited by 601 · see citing works
Recruitment to the Nuclear Periphery Can Alter Expression of Genes in Human Cells
Finlan, L. E., Sproul, D., Thomson, I., Boyle, S., Kerr, E. A., Perry, P., Ylstra, B., Chubb, J. R., & Bickmore, W. A. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000039 · Cited by 584 · see citing works
Röber, R.-A., Weber, K. K., & Osborn, M. (1989) · Development · doi:10.1242/dev.105.2.365 · Cited by 534 · see citing works
Zhang, J., Lian, Q., Zhu, G., Zhou, F., Lin, S., Tan, C., Mutalif, R., Navasankari, R., Zhang, Y., Tse, H., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.12.002 · Cited by 460 · see citing works
Liu, J., Ben-Shahar, T. R., Riemer, D., et al. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.11.3937 · Cited by 409 · see citing works
Lamin B1 is required for mouse development and nuclear integrity
Vergnes, L., Péterfy, M., Bergo, M. O., Young, S. G., & Reue, K. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0401424101 · Cited by 391 · see citing works
Kumaran, R. I., & Spector, D. L. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200706060 · Cited by 388 · see citing works
Stewart, C. L., & Burke, B. E. (1987) · Cell · doi:10.1016/0092-8674(87)90634-9 · Cited by 356 · see citing works
Detection of in vivo protein–DNA interactions using DamID in mammalian cells
Vogel, M. J., Peric‐Hupkes, D., & van Steensel, B. (2007) · Nature Protocols · doi:10.1038/nprot.2007.148 · Cited by 336 · see citing works
A Mitotic Lamin B Matrix Induced by RanGTP Required for Spindle Assembly
Tsai, M.-Y., Wang, S., Heidinger-Pauli, J. M., Shumaker, D. K., Adam, S. A., Goldman, R. D., & Zheng, Y. (2006) · Science · doi:10.1126/science.1122771 · Cited by 290 · see citing works
Self-citationA web-based tool for principal component and significance analysis of microarray data
Sharov, A. A., Dudekula, D. B., & Ko, M. S. H. (2005) · Computer applications in the biosciences · doi:10.1093/bioinformatics/bti343 · Cited by 242 · see citing works
Self-citationCoffinier, C., Jung, H.-J., Nobumori, C., Chang, S., Tu, Y., Barnes, R. H., Yoshinaga, Y., de Jong, P. J., Vergnes, L., Reue, K., et al. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-06-0504 · Cited by 225 · see citing works
Genome–nuclear lamina interactions and gene regulation
Kind, J., & van Steensel, B. (2010) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2010.04.002 · Cited by 195 · see citing works
Abnormal development of the cerebral cortex and cerebellum in the setting of lamin B2 deficiency
Coffinier, C., Chang, S. Y., Nobumori, C., Tu, Y., Farber, E., Toth, J. I., Fong, L. G., & Young, S. G. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0908790107 · Cited by 167 · see citing works
Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray
Carter, M. G., Sharov, A. A., VanBuren, V., Dudekula, D. B., Carmack, C. E., Nelson, C. N., & Ko, M. S. H. (2005) · Genome biology · doi:10.1186/gb-2005-6-7-r61 · Cited by 117 · see citing works
Self-citationRequirement for Nudel and dynein for assembly of the lamin B spindle matrix
Ma, L., Tsai, M.-Y., Wang, S., Lu, B., Chen, R., Yates, J. R., Zhu, X., & Zheng, Y. (2009) · Nature Cell Biology · doi:10.1038/ncb1832 · Cited by 113 · see citing works
Self-citation[50] Production of chimeras between embryonic stem cells and embryos
Stewart, C. L. (1993) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(93)25053-5 · Cited by 94 · see citing works
Yang, S. H., Chang, S. Y., Yin, L., Tu, Y., Hu, Y., Yoshinaga, Y., de Jong, P. J., Fong, L. G., & Young, S. G. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr266 · Cited by 90 · see citing works
A membranous spindle matrix orchestrates cell division
Zheng, Y. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2919 · Cited by 78 · see citing works
Self-citationOsouda, S., Nakamura, Y., Phalle, B. D. S., McConnell, M., Horigome, T., Sugiyama, S., Fisher, P. A., & Furukawa, K. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.05.022 · Cited by 69 · see citing works
Quinn, J., Kunath, T., & Rossant, J. (2005) · Humana Press eBooks · doi:10.1385/1-59259-983-4:123 · Cited by 48 · see citing works
Lamin B Counteracts the Kinesin Eg5 to Restrain Spindle Pole Separation during Spindle Assembly
Goodman, B. E., Channels, W. E., Qiu, M., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.140749 · Cited by 41 · see citing works
Gene structure and chromosomal localization of the murine lamin B2 gene.
Zewe, M., Höger, T. H., Fink, T. M., et al. (1991) · PubMed · Cited by 41 · see citing works
Yamada, M., Hirotsune, S., & Wynshaw-Boris, A. J. (2010) · Cell Adhesion & Migration · doi:10.4161/cam.4.2.10715 · Cited by 35 · see citing works
Genomic Structure of the Mouse Gene (Lmnb1) Encoding Nuclear Lamin B1
Maeno, H., Sugimoto, K., & Nakajima, N. (1995) · Genomics · doi:10.1006/geno.1995.9868 · Cited by 21 · see citing works
Cohen, T. V., & Stewart, C. L. (2008) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)00607-8 · Cited by 14 · see citing works
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- [15]Peric-Hupkes D Mol. Cell 2010 38 603 20513434 PMC5975946
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Lamina-Associated Domains: Links with Chromosome Architecture, Heterochromatin, and Gene Repression
van Steensel, B., & Belmont, A. S. (2017) · Cell · doi:10.1016/j.cell.2017.04.022 · Cited by 1,140 · see citing works
Lamin B1 loss is a senescence-associated biomarker
Freund, A., Laberge, R.-M., Demaria, M., & Campisi, J. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-10-0884 · Cited by 1,046 · see citing works
Solovei, I., Wang, A. S., Thanisch, K., Schmidt, C. S., Krebs, S., Zwerger, M., Cohen, T. V., Devys, D., Foisner, R., Peichl, L., et al. (2013) · Cell · doi:10.1016/j.cell.2013.01.009 · Cited by 809 · see citing works
Catastrophic nuclear envelope collapse in cancer cell micronuclei
Hatch, E. M., Fischer, A. H., Deerinck, T. J., & Hetzer, M. W. (2013) · Cell · doi:10.1016/j.cell.2013.06.007 · Cited by 741 · see citing works
Sadaie, M., Salama, R., Carroll, T., Tomimatsu, K., Chandra, T., Young, A. R. J., Narita, M., Pérez-Mancera, P. A., Bennett, D. C., Chong, H., et al. (2013) · Genes & Development · doi:10.1101/gad.217281.113 · Cited by 323 · see citing works
Saccon, T. D., Nagpal, R., Yadav, H., Cavalcante, M. B., Nunes, A. D. D. C., Schneider, A., Gesing, A., Hughes, B., Yousefzadeh, M., Tchkonia, T., et al. (2021) · The Journals of Gerontology: Series A · doi:10.1093/gerona/glab002 · Cited by 247 · see citing works
Chen, H., Zheng, X., Xiao, D., & Zheng, Y. (2016) · Aging Cell · doi:10.1111/acel.12465 · Cited by 107 · see citing works
Self-citationAlzheimer's disease: An acquired neurodegenerative laminopathy
Frost, B. (2016) · Nucleus · doi:10.1080/19491034.2016.1183859 · Cited by 74 · see citing works
- Authors
- Kim, Y., Sharov, A. A., McDole, K., Cheng, M., Hao, H., Fan, C.-M., Gaiano, N., Ko, M. S. H., & Zheng, Y.
- Affiliations
- Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA.
- National Institutes of Health (US)
- Howard Hughes Medical Institute (US)
- Johns Hopkins University (US)
- Corresponding author
- Ko, Minoru S. H.
- Zheng, Yixian
- Venue
- Science
- Published
- 2011-12-23
- Volume
- 334
- Issue
- 6063
- Pages
- 1706-1710
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3306219
- OpenAlex
- W2088969742
- Cited by
- 247
- References
- 35
- Citations by year
- 2026: 9 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 8 · 2020: 19 · 2019: 13 · 2018: 18 · 2017: 8 · 2016: 15 · 2015: 27 · 2014: 29 · 2013: 36 · 2012: 35
- FWCI
- 13.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health
- National Institute of General Medical Sciences (R01 GM 56312, R01GM056312)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR060042)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- B-type lamins, lamin B, nuclear lamina, organogenesis, mouse embryonic stem cells, self-renewal, pluripotency, gene silencing, trophectoderm, neural progenitor cells, spindle orientation, neuronal migration
- MeSH terms
- Animals, Brain, Cell Cycle, Cell Differentiation, Cell Movement, Cells, Cultured, Chromatin, Female, Male, Spindle Apparatus, Neurons, Organ Size, Promoter Regions, Genetic, Transcription, Genetic, Trophoblasts, Mice, Knockout, Gene Expression Regulation, Developmental, Gene Silencing, Nuclear Lamina, Lamin Type B, Organogenesis, Pluripotent Stem Cells, Embryonic Development, Body Size, Mice, Embryonic Stem Cells, Neural Stem Cells
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