Cited by 1,050Nuclear Structure and FunctionGenomics and Chromatin DynamicsChromosomal and Genetic Variations

Molecular Maps of the Reorganization of Genome-Nuclear Lamina Interactions during Differentiation

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Peric-Hupkes, Daan, Meuleman, Wouter, Pagie, Ludo, Bruggeman, Sophia W.M., Solovei, Irina, Brugman, Wim, Gräf, Stefan, Flicek, Paul +5 more·2010·Molecular Cell

Abstract

The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes. This reveals that a basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. This remodeling involves both individual transcription units and multigene regions and affects many genes that determine cellular identity. Often, genes that move away from the lamina are concomitantly activated; many others, however, remain inactive yet become unlocked for activation in a next differentiation step. These results suggest that lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation.

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Crossref · 10.1016/j.molcel.2010.03.016

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Authors
Peric-Hupkes, D., Meuleman, W., Pagie, L., Bruggeman, S. W. M., Solovei, I., Brugman, W., Gräf, S., Flicek, P., Kerkhoven, R. M., van Lohuizen, M., Reinders, M., Wessels, L., & van Steensel, B.
Affiliations
  • The Netherlands Cancer Institute (NL)
  • Ludwig-Maximilians-Universität München (DE)
  • European Bioinformatics Institute (GB)
  • Delft University of Technology (NL)
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2010-05
Volume
38
Issue
4
Pages
603-613
ISSN
1097-2765 (ISSN-L)
OpenAlex
W1984340155
Cited by
1,050
References
43
Citations by year
2026: 39 · 2025: 53 · 2024: 52 · 2023: 46 · 2022: 79 · 2021: 65 · 2020: 75 · 2019: 97 · 2018: 68 · 2017: 81 · 2016: 71 · 2015: 90 · 2014: 58 · 2013: 70 · 2012: 50
FWCI
22.78
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
Wellcome Trust (062023)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
nuclear lamina, chromosome organization, 3D genome organization, mouse embryonic stem cells, astrocyte differentiation, neural progenitor cells, lineage commitment, terminal differentiation, gene expression regulation, chromatin remodeling, cell identity genes
MeSH terms
Animals, Astrocytes, Cell Differentiation, Drosophila, Humans, Neurons, Genome, Cell Lineage, Nuclear Lamina, Chromosome Positioning, Mice, Embryonic Stem Cells

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