Endogenous Protein S-Nitrosylation in E. coli : Regulation by OxyR
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Handling Nitrosative Stress: S-nitrosylation of proteins is a principal mechanism of cellular signaling in eukaryotes but has not been reported in microbes. The transcription factor OxyR serves to protect bacterial cells from reactive oxygen species produced by cell metabolism in the presence of oxygen. Seth et al. (p. 470) found that when cells grew in the presence of nitrate, OxyR was modified by S-nitrosylation of the same cysteine residue that gets oxidized in cells grown aerobically. However, the nitrosylated OxyR activated a different set of genes—some of which appeared to protect the cell from excessive S-nitrosylation.
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Oral bacteria could directly catalyze nitrosation reactions from nitrate without intermediate nitrite formation, as protein S-nitros(yl)ation is an obligate concomitant process of anaerobic respiration on nitrate in E. coli.
“However, one cannot entirely rule out the possibility that oral bacteria could directly catalyze nitrosation reactions from nitrate without intermediate nitrite formation because recent data show that protein S-nitros(yl)ation is an obligate concomitant process of anaerobic respiration on nitrate in Escherichia coli.43”
From Dietary nitrate supplementation improves revascularization in chronic ischemia (2012) · cites this paper as [43]
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- Authors
- Seth, D., Hausladen, A., Wang, Y.-J., & Stamler, J. S.
- Affiliations
- University Hospitals Cleveland Medical Center (US)
- Center for Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA.
- Corresponding author
- Stamler, Jonathan S.
- Venue
- Science
- Published
- 2012-04-27
- Volume
- 336
- Issue
- 6080
- Pages
- 470-473
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3837355
- OpenAlex
- W2074431736
- Cited by
- 158
- References
- 32
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 11 · 2020: 9 · 2019: 13 · 2018: 10 · 2017: 8 · 2016: 14 · 2015: 20 · 2014: 19 · 2013: 20 · 2012: 13
- FWCI
- 9.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL095463, R01HL059130, R01HL096673)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- S-nitrosylation, OxyR, Escherichia coli, anaerobic nitrate respiration, transcriptional regulation, S-nitrosothiols, post-translational modification, transcriptional regulon
- MeSH terms
- Anaerobiosis, Culture Media, Nitrates, Nitric Oxide, Nitrites, Oxidation-Reduction, Promoter Regions, Genetic, Protein Processing, Post-Translational, Repressor Proteins, Transcriptional Activation, Regulon, S-Nitrosothiols, Escherichia coli Proteins, Escherichia coli K12
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