Cited by 285Advanced biosensing and bioanalysis techniquesDNA and Biological ComputingDNA and Nucleic Acid Chemistry

Rapid Folding of DNA into Nanoscale Shapes at Constant Temperature

Sobczak, Jean-Philippe J., Martin, Thomas G., Gerling, Thomas, Dietz, Hendrik·2012·Science

Abstract

Speeding Up Nanoscale DNA Assembly: An impressive array of three-dimensional (3D) nanoscale objects has been assembled by folding a long, single-stranded DNA scaffold by binding of short DNA staples. However, these processes tend to be slow and inefficient. Sobczak et al. (p. 1458 ) examined the folding process with an intercalating fluorescence dye to measure the formation of double-stranded DNA in folding processes or single-stranded DNA in unfolding. Reaction conditions were identified that sped up folding by orders of magnitude and increased yields of the 3D nanoscale objects to nearly 100%.

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Registry

Crossref · 10.1126/science.1229919

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29 of 29 references verified0 citation errors · 0 not checkable

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  • Because of the noncovalent nature of DNA nanostructures, they can be reconfigured using established strategies, including strand displacement, thermal annealing, and pH changes.

    “Because of the noncovalent nature of DNA nanostructures, they can be reconfigured using established strategies, including strand displacement,26 thermal annealing,105 and pH changes.106”

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Authors
Sobczak, J.-P. J., Martin, T. G., Gerling, T., & Dietz, H.
Affiliations
Technical University of Munich (DE)
Corresponding author
Dietz, Hendrik
Venue
Science
Published
2012-12-14
Volume
338
Issue
6113
Pages
1458-1461
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2049040320
Cited by
285
References
29
Citations by year
2026: 9 · 2025: 13 · 2024: 17 · 2023: 18 · 2022: 18 · 2021: 13 · 2020: 15 · 2019: 20 · 2018: 25 · 2017: 24 · 2016: 28 · 2015: 26 · 2014: 42 · 2013: 15 · 2012: 2
FWCI
10.92
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
DNA folding, DNA nanotechnology, cooperative folding, nucleation, DNA origami, folding kinetics, DNA topology
MeSH terms
DNA, Nucleic Acid Conformation, Temperature, Templates, Genetic, Protein Folding, Nanotechnology, Nanostructures

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