Rapid Folding of DNA into Nanoscale Shapes at Constant Temperature
Abstract
Speeding Up Nanoscale DNA Assembly: An impressive array of three-dimensional (3D) nanoscale objects has been assembled by folding a long, single-stranded DNA scaffold by binding of short DNA staples. However, these processes tend to be slow and inefficient. Sobczak et al. (p. 1458 ) examined the folding process with an intercalating fluorescence dye to measure the formation of double-stranded DNA in folding processes or single-stranded DNA in unfolding. Reaction conditions were identified that sped up folding by orders of magnitude and increased yields of the 3D nanoscale objects to nearly 100%.
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Because of the noncovalent nature of DNA nanostructures, they can be reconfigured using established strategies, including strand displacement, thermal annealing, and pH changes.
“Because of the noncovalent nature of DNA nanostructures, they can be reconfigured using established strategies, including strand displacement,26 thermal annealing,105 and pH changes.106”
From Emerging Approaches to DNA Data Storage: Challenges and Prospects (2022) · cites this paper as [105]
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- Authors
- Sobczak, J.-P. J., Martin, T. G., Gerling, T., & Dietz, H.
- Affiliations
- Technical University of Munich (DE)
- Corresponding author
- Dietz, Hendrik
- Venue
- Science
- Published
- 2012-12-14
- Volume
- 338
- Issue
- 6113
- Pages
- 1458-1461
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2049040320
- Cited by
- 285
- References
- 29
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 17 · 2023: 18 · 2022: 18 · 2021: 13 · 2020: 15 · 2019: 20 · 2018: 25 · 2017: 24 · 2016: 28 · 2015: 26 · 2014: 42 · 2013: 15 · 2012: 2
- FWCI
- 10.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA folding, DNA nanotechnology, cooperative folding, nucleation, DNA origami, folding kinetics, DNA topology
- MeSH terms
- DNA, Nucleic Acid Conformation, Temperature, Templates, Genetic, Protein Folding, Nanotechnology, Nanostructures
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