The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Total Synthesis of a Functional Designer Eukaryotic Chromosome
Open accessAbstract
Designer Chromosome: One of the ultimate aims of synthetic biology is to build designer organisms from the ground up. Rapid advances in DNA synthesis has allowed the assembly of complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge to synthetic biologists. Annaluru et al. (p. 55 , published online 27 March) designed a synthetic eukaryotic chromosome based on yeast chromosome III. The designer chromosome, shorn of destabilizing transfer RNA genes and transposons, is ∼14% smaller than its wild-type template and is fully functional with every gene tagged for easy removal.
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Cites
Programming cells by multiplex genome engineering and accelerated evolution
Wang, H. H., Isaacs, F. J., Carr, P. A., Sun, Z. Z., Xu, G., Forest, C. R., & Church, G. M. (2009) · Nature · doi:10.1038/nature08187 · Cited by 1,529
The distribution of the numbers of mutants in bacterial populations
Lea, D. E., & Coulson, C. A. (1949) · Journal of Genetics · doi:10.1007/bf02986080 · Cited by 1,450
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A genome-wide view of the spectrum of spontaneous mutations in yeast.
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Cohesin relocation from sites of chromosomal loading to places of convergent transcription
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Cited by
Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes
Mus, F., Crook, M. B., Garcia, K., Garcia Costas, A., Geddes, B. A., Kouri, E. D., Paramasivan, P., Ryu, M.-H., Oldroyd, G. E. D., Poole, P. S., et al. (2016) · Applied and Environmental Microbiology · doi:10.1128/aem.01055-16 · Cited by 593
Total synthesis of Escherichia coli with a recoded genome
Fredens, J., Wang, K., de la Torre, D., Funke, L. F. H., Robertson, W. E., Christova, Y., Chia, T., Schmied, W. H., Dunkelmann, D. L., Beránek, V., et al. (2019) · Nature · doi:10.1038/s41586-019-1192-5 · Cited by 526
Mans, R., van Rossum, H. M., Wijsman, M., Backx, A., Kuijpers, N. G. A., van den Broek, M., Daran-Lapujade, P., Pronk, J. T., van Maris, A. J. A., & Daran, J.-M. G. (2015) · FEMS Yeast Research · doi:10.1093/femsyr/fov004 · Cited by 485
Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology
Hughes, R. A., & Ellington, A. D. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a023812 · Cited by 411
Materials design by synthetic biology
Tang, T.-C., An, B., Huang, Y., Vasikaran, S., Wang, Y., Jiang, X., Lu, T. K., & Zhong, C. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-00265-w · Cited by 386
Metadata
- Authors
- Annaluru, N., Muller, H., Mitchell, L. A., Ramalingam, S., Stracquadanio, G., Richardson, S. M., Dymond, J. S., Kuang, Z., Scheifele, L. Z., Cooper, E. M., Cai, Y., Zeller, K., Agmon, N., Han, J. S., Hadjithomas, M., Tullman, J., Caravelli, K., Cirelli, K., Guo, Z., London, V., Yeluru, A., Murugan, S., Kandavelou, K., Agier, N., Fischer, G., Yang, K., Martin, J. A., Bilgel, M., Bohutskyi, P., Boulier, K. M., Capaldo, B. J., Chang, J., Charoen, K., Choi, W. J., Deng, P., DiCarlo, J. E., Doong, J., Dunn, J., Feinberg, J. I., Fernandez, C., Floria, C. E., Gladowski, D., Hadidi, P., Ishizuka, I., Jabbari, J., Lau, C. Y. L., Lee, P. A., Li, S., Lin, D., Linder, M. E., Ling, J., Liu, J., Liu, J., London, M., Ma, H., Mao, J., McDade, J. E., McMillan, A., Moore, A. M., Oh, W. C., Ouyang, Y., Patel, R., Paul, M., Paulsen, L. C., Qiu, J., Rhee, A., Rubashkin, M. G., Soh, I. Y., Sotuyo, N. E., Srinivas, V., Suarez, A., Wong, A., Wong, R., Xie, W. R., Xu, Y., Yu, A. T., Koszul, R., Bader, J. S., Boeke, J. D., & Chandrasegaran, S.
- Venue
- Science
- Published
- 2014-04-04
- Volume
- 344
- Issue
- 6179
- Pages
- 55-58
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2124208385
- Cited by
- 545
- References
- 5
- FWCI
- 29.08
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Total Synthesis of a Functional Designer Eukaryotic Chromosome. https://app.cochat.ai/papers/10.1126/science.1249252
- The paper
- Annaluru, N., Muller, H., Mitchell, L. A., Ramalingam, S., Stracquadanio, G., Richardson, S. M., Dymond, J. S., Kuang, Z., Scheifele, L. Z., Cooper, E. M., Cai, Y., Zeller, K., Agmon, N., Han, J. S., Hadjithomas, M., Tullman, J., Caravelli, K., Cirelli, K., Guo, Z., . . . Chandrasegaran, S. (2014). Total Synthesis of a Functional Designer Eukaryotic Chromosome. Science. https://doi.org/10.1126/science.1249252
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
