Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins
Abstract
High-definition view of the synapse: Individual neurons communicate with one another via their synapses, so to understand the nervous system, we need to understand in detail how the synapses are organized. Wilhelm et al. present a quantitative molecular-scale image of the “average” synapse populated with realistic renditions of each of the protein components that contribute to the inner workings of neurons. Science , this issue p. 1023
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APP is highly expressed in neurons and abundant at the synapse.
“APP is a type I transmembrane protein and is highly expressed in neurons and abundant at the synapse (48–52).”
From The β-Secretase BACE1 in Alzheimer’s Disease (2021) · cites this paper as [52]
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- Authors
- Wilhelm, B. G., Mandad, S., Truckenbrodt, S., Kröhnert, K., Schäfer, C., Rammner, B., Koo, S. J., Claßen, G. A., Krauss, M., Haucke, V., Urlaub, H., & Rizzoli, S. O.
- Affiliations
- Universitätsmedizin Göttingen (DE)
- Max Planck Institute for Biophysical Chemistry (DE)
- Leibniz-Forschungsinstitut für Molekulare Pharmakologie (DE)
- Corresponding author
- Mandad, Sunit
- Truckenbrodt, Sven
- Rizzoli, Silvio O.
- ORCID
- Venue
- Science
- Published
- 2014-05-30
- Volume
- 344
- Issue
- 6187
- Pages
- 1023-1028
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1984678540
- Semantic Scholar
- 473f04ac1f32c55d17e49f35388b6a55be361804
- Cited by
- 770
- References
- 5
- Citations by year
- 2026: 28 · 2025: 49 · 2024: 59 · 2023: 65 · 2022: 77 · 2021: 82 · 2020: 74 · 2019: 75 · 2018: 65 · 2017: 52 · 2016: 61 · 2015: 62 · 2014: 21
- FWCI
- 32.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Digital Library (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- synaptic vesicle recycling, synaptic boutons, protein copy number, synaptic proteome, exocytosis, endosomal membrane fusion, synaptic protein localization
- MeSH terms
- Animals, Brain, Exocytosis, Microscopy, Electron, Models, Neurological, Mass Spectrometry, Synaptic Vesicles, Synaptosomes, Immunoblotting, Rats, Wistar, Presynaptic Terminals, Protein Transport, Imaging, Three-Dimensional, Vesicular Transport Proteins, Rats
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