Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3
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Vesicular transporters (VTs) define the type of neurotransmitter that synaptic vesicles (SVs) store and release. While certain mammalian neurons release multiple transmitters, it is not clear whether the release occurs from the same or distinct vesicle pools at the synapse. Using quantitative single-vesicle imaging, we show that a vast majority of SVs in the rodent brain contain only one type of VT, indicating specificity for a single neurotransmitter. Interestingly, SVs containing dual transporters are highly diverse (27 types) but small in proportion (2% of all SVs), excluding the largest pool that carries VGLUT1 and ZnT3 (34%). Using VGLUT1-ZnT3 SVs, we demonstrate that the transporter colocalization influences the SV content and synaptic quantal size. Thus, the presence of diverse transporters on the same vesicle is bona fide, and depending on the VT types, this may act to regulate neurotransmitter type, content, and release in space and time.
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Complementary dependencies may explain observed synergies between vGluT and VAChT or vGluT and ZnT3 on the same synaptic vesicle.
“These complementary dependencies may explain the observed synergies between vGluT and VAChT or vGluT and the vesicular zinc transporter (ZnT3) when these are localized to the same synaptic vesicle.5,21,22”
From Synaptic and circuit functions of multitransmitter neurons in the mammalian brain (2023) · cites this paper as [5]
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ATG9 resides on a unique population of small vesicles in presynaptic nerve terminals
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MINFLUX fluorescence nanoscopy in biological tissue
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Synaptic zinc potentiates AMPA receptor function in mouse auditory cortex
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Novel Genes Involved in Hypertrophic Cardiomyopathy: Data of Transcriptome and Methylome Profiling
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On the genesis and unique functions of zinc neuromodulation
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Zinc Transporters in Diseases, Including Diabetes and Related Conditions
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Metal ion content of internal organs in the calorically restricted Wistar rat
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Role of adaptor protein complexes in generating functionally distinct synaptic vesicle pools
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ArtiaX: geometric models, camera paths and image processing tools
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From Blur to Brilliance: The Ascendance of Advanced Microscopy in Neuronal Cell Biology
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Acetylcholine Neurons Become Cholinergic during Three Time Windows in the Developing Mouse Brain
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Lower Zinc but Higher Calcium Content in Rodent Spinal Cord Compared to Brain
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Prevalent co-release of glutamate and GABA throughout the mouse brainPreprint
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OpenSTED: open-source dynamic intensity minimum system for stimulated emission depletion microscopy
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A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles
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Strategies and tactics for subcellular targeting of small molecule zinc sensors
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Synaptic summation shapes information transfer in GABA-glutamate co-transmission
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Mesoscopic analysis of GABAergic marker expression in acetylcholine neurons in the whole mouse brain
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An Ultraresponsive Green Biosensor for Robust in Vivo Imaging of Synaptic Zinc Dynamics
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Pharmacological inhibition of V-ATPase targets mode-switching but not the proton transport cyclePreprint
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Elucidation of the zinc binding site in KCNQ channels
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hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamicsPreprint
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Electrophysiology in nanoscale compartments
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Does SLC17A9 (VNUT) function as a stand-alone nucleotide and/or phosphate transporter?
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Self-citationA microfluidics platform for studying ion and solute transport by isolated synaptic vesicles
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- Authors
- Upmanyu, N., Jin, J., Emde, H. V. D., Ganzella, M., Bösche, L., Malviya, V. N., Zhuleku, E., Politi, A. Z., Ninov, M., Silbern, I., Leutenegger, M., Urlaub, H., Riedel, D., Preobraschenski, J., Milosevic, I., Hell, S. W., Jahn, R., & Sambandan, S.
- Affiliations
- Max Planck Institute for Multidisciplinary Sciences (DE)
- Max Planck Society (DE)
- Universitätsmedizin Göttingen (DE)
- Max Planck Institute for Medical Research (DE)
- Corresponding author
- Sambandan, Sivakumar
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2022-05
- Volume
- 110
- Issue
- 9
- Pages
- 1483-1497.e7
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W4221118099
- Cited by
- 69
- References
- 77
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 24 · 2023: 16 · 2022: 8
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Neuron (publishedVersion, cc-by)
- PubMed (publishedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
- Funders
- Broad Institute
- Deutsche Forschungsgemeinschaft (EXC 2067, SFB1286, EXC 2067‐1, 390729940, EXC 2067/1390729940)
- Volkswagen Foundation
- Bundesministerium für Bildung und Forschung
- Fraunhofer-Gesellschaft
- Max-Planck-Gesellschaft
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- synaptic vesicles, VGLUT1, ZnT3, vesicle content, neurotransmitter release, single-vesicle imaging
- MeSH terms
- Animals, Mammals, Synapses, Synaptic Vesicles, Neurotransmitter Agents, Membrane Transport Proteins, Neurotransmitter Transport Proteins, Vesicular Glutamate Transport Protein 1
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