Reconstitution of I KATP : An Inward Rectifier Subunit Plus the Sulfonylurea Receptor
Abstract
A member of the inwardly rectifying potassium channel family was cloned here. The channel, called BIR (Kir6.2), was expressed in large amounts in rat pancreatic islets and glucose-responsive insulin-secreting cell lines. Coexpression with the sulfonylurea receptor SUR reconstituted an inwardly rectifying potassium conductance of 76 picosiemens that was sensitive to adenosine triphosphate (ATP) ( I KATP ) and was inhibited by sulfonylureas and activated by diazoxide. The data indicate that these pancreatic β cell potassium channels are a complex composed of at least two subunits-BIR, a member of the inward rectifier potassium channel family, and SUR, a member of the ATP-binding cassette superfamily. Gene mapping data show that these two potassium channel subunit genes are clustered on human chromosome 11 at position 11p15.1.
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The KATP channel is an octameric complex of 4 pore-forming Kir6.2 subunits and 4 regulatory SUR1 subunits.
“The successful cloning and molecular characterization of the KATP channel revealed it is an octameric complex of 4 pore-forming Kir6.2 s and 4 regulatory SUR1 subunits (4, 302, 583).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [302]
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- Authors
- Inagaki, N., Gonoi, T., Clement, J. P., Namba, N., Inazawa, J., Gonzalez, G., Aguilar-Bryan, L., Seino, S., & Bryan, J.
- Affiliations
- Chiba University (JP)
- Baylor College of Medicine (US)
- Kyoto Prefectural University of Medicine (JP)
- J. P. Clement IV, G. Gonzalez, L. Aguilar-Bryan, and J. Bryan are in the Departments of Cell Biology and Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
- Corresponding author
- Inagaki, Nobuya
- Gonoi, Tohru
- Seino, Susumu
- Venue
- Science
- Published
- 1995-11-17
- Volume
- 270
- Issue
- 5239
- Pages
- 1166-1170
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2083584165
- Cited by
- 1,709
- References
- 1
- Citations by year
- 2026: 11 · 2025: 15 · 2024: 26 · 2023: 14 · 2022: 21 · 2021: 29 · 2020: 14 · 2019: 30 · 2018: 25 · 2017: 29 · 2016: 28 · 2015: 40 · 2014: 30 · 2013: 52 · 2012: 45
- FWCI
- 37.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (DK44311, R01DK044311)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- ATP-sensitive potassium channels, Kir6.2, sulfonylurea receptor, inwardly rectifying potassium channels, pancreatic beta cells, diazoxide, channel reconstitution, gene mapping, insulin secretion
- MeSH terms
- Adenosine Triphosphate, Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 11, Cloning, Molecular, Diazoxide, Cricetinae, Humans, Islets of Langerhans, Molecular Sequence Data, Potassium, Receptors, Drug, Rubidium, Sulfonylurea Compounds, Potassium Channels, ATP-Binding Cassette Transporters, Potassium Channels, Inwardly Rectifying, Mice, Rats, KATP Channels, Sulfonylurea Receptors
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