Recovery of replication-competent HIV despite prolonged suppression of plasma viremia
Abstract
In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new drug resistance mutations or discernible evolution, providing evidence for viral latency rather than drug failure.
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After entering a controlled, chronic phase of HIV-1 infection, the main viral reservoir of intact proviral genomes is expected to reside in memory T cell subsets that persist for years.
“after entering a controlled, chronic phase of HIV-1 infection, the main viral reservoir of intact proviral genomes is expected to reside in memory T cell subsets that persist for years. 10-12”
From Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods (2023) · cites this paper as [11]
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Self-citation
- Authors
- Wong, J. K., Hezareh, M., Günthard, H. F., Havlir, D. V., Ignacio, C. C., Spina, C. A., & Richman, D. D.
- Affiliations
- University of California San Diego (US)
- J. K. Wong, M. Hezareh, H. F. Günthard, C. C. Ignacio, C. A. Spina, Departments of Medicine and Pathology, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093, USA.
- Corresponding author
- Wong, Joseph K.
- Venue
- Science
- Published
- 1997-11-14
- Volume
- 278
- Issue
- 5341
- Pages
- 1291-1295
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2090646713
- Cited by
- 2,186
- References
- 1
- Citations by year
- 2026: 28 · 2025: 66 · 2024: 57 · 2023: 81 · 2022: 66 · 2021: 69 · 2020: 112 · 2019: 95 · 2018: 76 · 2017: 75 · 2016: 80 · 2015: 79 · 2014: 58 · 2013: 74 · 2012: 53
- FWCI
- 39.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI38858, AI 01361, U01AI038858, U01AI027670, AI2-7670)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Keywords
- HIV, viral reservoir, viral latency, peripheral blood mononuclear cells, drug resistance mutations, HIV evolution
- MeSH terms
- Drug Resistance, Microbial, Drug Therapy, Combination, Humans, Immunologic Memory, Lymphocyte Activation, Mutation, RNA, Viral, Viremia, Virus Activation, Virus Replication, Zidovudine, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, T-Lymphocyte Subsets, Virus Latency, Coculture Techniques, Lamivudine, Anti-HIV Agents, Indinavir, Viral Load
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