Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases
Abstract
The complement and immunoglobulin receptors are the major phagocytic receptors involved during infection. However, only immunoglobulin-dependent uptake results in a respiratory burst and an inflammatory response in macrophages. Rho guanosine triphosphatases (molecular switches that control the organization of the actin cytoskeleton) were found to be essential for both types of phagocytosis. Two distinct mechanisms of phagocytosis were identified: Type I, used by the immunoglobulin receptor, is mediated by Cdc42 and Rac, and type II, used by the complement receptor, is mediated by Rho. These results suggest a molecular basis for the different biological consequences that are associated with phagocytosis.
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Actin assembly during phagocytosis is mediated by Rho family GTPases.
“Experiments using clostridial toxins or transfection of dominant-negative constructs have convincingly demonstrated that actin assembly during phagocytosis is mediated by Rho family GTPases (Cox et al. 1997; Hackam et al. 1997; Caron and Hall 1998; Massol et al. 1998).”
From Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis (2000) · cites this paper as Caron and Hall 1998
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- Authors
- Caron, E., & Hall, A.
- Affiliations
- MRC Laboratory for Molecular Cell Biology (GB)
- Corresponding author
- Hall, Alan
- Venue
- Science
- Published
- 1998-11-27
- Volume
- 282
- Issue
- 5394
- Pages
- 1717-1721
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2088618181
- Cited by
- 1,019
- References
- 1
- Citations by year
- 2026: 9 · 2025: 16 · 2024: 8 · 2023: 13 · 2022: 25 · 2021: 19 · 2020: 24 · 2019: 26 · 2018: 20 · 2017: 32 · 2016: 33 · 2015: 34 · 2014: 40 · 2013: 38 · 2012: 36
- FWCI
- 19.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- phagocytosis, Rho GTPases, Cdc42, Rac, Rho, complement receptors, immunoglobulin receptors, macrophages, actin cytoskeleton, respiratory burst, inflammation, phagocytic receptors
- MeSH terms
- Actins, Animals, Bacterial Proteins, Bacterial Toxins, Cell Line, Enzyme Activation, Erythrocytes, Macrophages, Opsonin Proteins, Phagocytosis, Phagosomes, Transfection, Antigens, CD, Macrophage-1 Antigen, 3T3 Cells, Receptors, IgG, Cell Cycle Proteins, GTP-Binding Proteins, COS Cells, GTP Phosphohydrolases, rac GTP-Binding Proteins, cdc42 GTP-Binding Protein, Mice
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