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Phosphorylation and Regulation of Raf by Akt (Protein Kinase B)

Zimmermann, Sven, Moelling, Karin·1999·Science

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Abstract

Activation of the protein kinase Raf can lead to opposing cellular responses such as proliferation, growth arrest, apoptosis, or differentiation. Akt (protein kinase B), a member of a different signaling pathway that also regulates these responses, interacted with Raf and phosphorylated this protein at a highly conserved serine residue in its regulatory domain in vivo. This phosphorylation of Raf by Akt inhibited activation of the Raf-MEK-ERK signaling pathway and shifted the cellular response in a human breast cancer cell line from cell cycle arrest to proliferation. These observations provide a molecular basis for cross talk between two signaling pathways at the level of Raf and Akt.

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Authors: Zimmermann, S., & Moelling, K.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1999-11-26
Volume: 286
Issue: 5445
Pages: 1741-1744
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.286.5445.1741
OpenAlex: W1971727526
Cited by: 1,078
References: 1
FWCI: 22.94
Type: journal article
Language: en
Open access: closed

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Phosphorylation and Regulation of Raf by Akt (Protein Kinase B)

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