The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Localization of the G Protein βγ Complex in Living Cells During Chemotaxis
Abstract
Gradients of chemoattractants elicit signaling events at the leading edge of a cell even though chemoattractant receptors are uniformly distributed on the cell surface. In highly polarized Dictyostelium discoideum amoebas, membrane-associated βγ subunits of heterotrimeric guanine nucleotide–binding proteins (G proteins) were localized in a shallow anterior-posterior gradient. A uniformly applied chemoattractant generated binding sites for pleckstrin homology (PH) domains on the inner surface of the membrane in a pattern similar to that of the Gβγ subunits. Loss of cell polarity resulted in uniform distribution of both the Gβγ subunits and the sensitivity of PH domain recruitment. These observations indicate that Gβγ subunits are not sufficiently localized to restrict signaling events to the leading edge but that their distribution may determine the relative chemotactic sensitivity of polarized cells.
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Cites
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Self-citationNew roles for G-protein (βγ-dimers in transmembrane signalling
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Self-citationG Protein Signaling Events Are Activated at the Leading Edge of Chemotactic Cells
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Self-citationLeukocyte polarization in cell migration and immune interactions
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Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
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Vanhaesebroeck, B., Leevers, S. J., Ahmadi, K., Timms, J., Katso, R., Driscoll, P. C., Woscholski, R., Parker, P. J., & Waterfield, M. D. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.535 · Cited by 1,535
Tumor Suppressor PTEN Mediates Sensing of Chemoattractant Gradients
Iijima, M., & Devreotes, P. (2002) · Cell · doi:10.1016/s0092-8674(02)00745-6 · Cited by 677
Self-citationNeuronal polarity: from extracellular signals to intracellular mechanisms.
Arimura, N., & Kaibuchi, K. (2007) · Nature Reviews Neuroscience · doi:10.1038/nrn2056 · Cited by 655
Swaney, K. F., Huang, C.-H., & Devreotes, P. N. (2010) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.093008.131228 · Cited by 494
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Metadata
- Authors
- Jin, T., Zhang, N., Long, Y., Parent, C. A., & Devreotes, P. N.
- Venue
- Science
- Published
- 2000-02-11
- Volume
- 287
- Issue
- 5455
- Pages
- 1034-1036
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2038678974
- Cited by
- 277
- References
- 20
- FWCI
- 9.46
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jin, T., Zhang, N., Long, Y., Parent, C. A., & Devreotes, P. N. (2000). Localization of the G Protein βγ Complex in Living Cells During Chemotaxis. Science. https://doi.org/10.1126/science.287.5455.1034
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
